The origin of osteoclasts: An immunohistochemical study on macrophages and osteoclasts in embryonic rat bone (original) (raw)
Summary
The origin of osteoclasts was studied in embryonic rat bone primordia using a set of monoclonal antibodies (ED1, ED2, and ED3) that exclusively recognize monocytes and macrophages. ED1 recognizes monocytes and macrophages. Mononuclear phagocytes which were ED1 positive were found in the perichondrium/periosteum of developing bone. These cells started to infiltrate the primordia when the cartilage became hypertrophic. During bone formation, multinucleated ED1-positive cells with the morphological characteristics of osteoclasts were found in the developing bone marrow cavity and against the bone collar. The present findings support the notion that osteoclasts arise by fusion of mononuclear phagocytes derived from blood monocytes.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Chambers TJ (1985) The pathobiology of the osteoclast. J Clin Pathol 38:241–252
PubMed CAS Google Scholar - Jotereau FV, Le Douarin NM (1978) The developmental relationship between osteocytes and osteoclasts; a study using the quail-chick nuclear marker in enchondral ossification. Dev Biol 63:253–265
Article PubMed CAS Google Scholar - Loutit JF, Nisbet NW (1982) The origin of osteoclasts. Immunobiol 161:193–203
CAS Google Scholar - Marks SC (1983) The origin of osteoclasts. J Pathol 12:226–256
Google Scholar - Thesingh CW, Burger EH (1983) The role of mesenchyme in embryonic long bones as early deposition site for osteoclast progenitor cells. Dev Biol 95:429–438
Article PubMed CAS Google Scholar - Burger EH, Van der Meer JWM, Van de Gevel JS, Gribnau JC, Thesingh CW, Van Furth R (1982) In vitro formation of osteoclasts from long-term cultures of bone marrow mononuclear phagocytes. J Exp Med 136:1604–1614
Article Google Scholar - Hume DA, Loutit JF, Gordon S (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: macrophages of bone and associated connective tissue. J Cell Sci 66:189–194
PubMed CAS Google Scholar - Horton MA, Rimmer EF, Moore A, Chambers TJ (1985) On the origin of the osteoclast: the cell surface phenotype of rodent osteoclasts. Calcif Tissue Int 37:46–50
PubMed CAS Google Scholar - Nijweide PJ, Vrijheid-Lammers T, Mulder RJP, Blok J (1985) Cell surface antigens on osteoclasts and related cells in the quail studied with monoclonal antibodies. Histochemistry 83:315–324
Article PubMed CAS Google Scholar - Dijkstra CD, Döpp ED, Joling P, Kraal G (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 54:589–599
PubMed CAS Google Scholar - Pearse, AGE (1968) Histochemistry. Theoretical and Applied, vol. 1, 3rd ed. Churchill Livingstone, Edinburgh
Google Scholar
Author information
Authors and Affiliations
- Department of Histology, Medical Faculty, Free University, P.O. Box 7161, 1007 MC, Amsterdam, The Netherlands
Taede Sminia & Christine D. Dijkstra
Authors
- Taede Sminia
You can also search for this author inPubMed Google Scholar - Christine D. Dijkstra
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Sminia, T., Dijkstra, C.D. The origin of osteoclasts: An immunohistochemical study on macrophages and osteoclasts in embryonic rat bone.Calcif Tissue Int 39, 263–266 (1986). https://doi.org/10.1007/BF02555216
- Received: 27 December 1985
- Revised: 14 April 1986
- Issue Date: July 1986
- DOI: https://doi.org/10.1007/BF02555216