Morphological differences and molecular similarities between Paragonimus bangkokensis and P. harinasutai (original) (raw)

Abstract

Paragonimus bangkokensis and Paragonimus harinasutai were found one after another in the same crab host, Potamon smithianus, in Thailand in 1967 and 1968. Both species were also recently found in China and Lao People's Democratic Republic (PDR). Those two Paragonimus spp. are distinguishable from each other by morphological features of metacercariae and adults. However, recently, the DNA sequences of second internal transcribed spacer region (ITS2) and cytochrome oxidase subunit 1 (CO1) genes of those two species in Thailand were reported to be highly similar to each other. In the present study, we collected P. bangkokensis in two provinces in Vietnam (the first record in Vietnam) and both P. bangkokensis and P. harinasutai in Lao PDR for the morphological and molecular phylogenetic analyses to clarify the mutual relationship between the two species. The results show that P. bangkokensis and P. harinasutai were distinguishable from each other by morphology such as the size of metacercariae and the arrangement of cuticular spines of adult worms. However, the molecular phylogenetic analyses of ITS2 and CO1 genes clearly indicate that P. bangkokensis and P. harinasutai make a monophyletic group.

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Acknowledgements

This study was partially supported by the Japanese Society for Promotion of Science (JSPS) under Ronpaku Fellowship no. NCST-10430 for Pham Ngoc Doanh, and by the Project for Zoonosis Education and Research, University of Miyazaki.

Author information

Authors and Affiliations

1. Department of Parasitology, Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
   Pham Ngoc Doanh
2. Department of Bio-resources, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
   Akio Shinohara
3. Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki, 889-2192, Japan
   Yoichiro Horii
4. Sigma Clinic, Fukuoka, Japan
   Shinichiro Yahiro
5. Department of Microbiology and Immunology, School of Medicine, Fukuoka University, Fukuoka, Japan
   Shigehisa Habe
6. Institut de la Francophonie pour la Medicine Tropicale, Vientiane, Laos
   Nanthasane Vannavong & Michel Strobel
7. Research Institute, International Medical Center of Japan, Tokyo, Japan
   Satoshi Nakamura
8. Division of Parasitology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan
   Yukifumi Nawa

Authors

1. Pham Ngoc Doanh
2. Akio Shinohara
3. Yoichiro Horii
4. Shinichiro Yahiro
5. Shigehisa Habe
6. Nanthasane Vannavong
7. Michel Strobel
8. Satoshi Nakamura
9. Yukifumi Nawa

Corresponding author

Correspondence toYoichiro Horii.

Appendix

Appendix

Alternative maximum likelihood tree obtained from CO1 data set; (AB270677 P. heterotremus ((AB270683 P. vietnamensis (((U97214 P. ohirai, AF008189 P. ohirai, AF008190 P. sadoensis), AF008188 P. iloktsuenensis), (((((AF159600 P. harinasutai, AB354226 P. harinasutai), (AB354227 P. bangkokensis (AD-SH1, AD-SH2 (AD-SH4, MC-SH1, MC-SH2), AD-SH5, AD-SH6, MC-SH3))), ((AB354228 P. bangkokensis (MC-LAO4, MC-LAO5, MC-LAO6, AD-LAO2, AD-LAO4)), (MC-LAO3, MC-LAO2, MC-LAO1, AD-LAO1))), (((AD-SH7, MC-SH4, MC-SH5), MC-SH6), (MM-QN1, MM-QN2, MM-QN3, MM-QN4))), AB354604 P. harinasutai))), AB355663 P. proliferus))

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Ngoc Doanh, P., Shinohara, A., Horii, Y. et al. Morphological differences and molecular similarities between Paragonimus bangkokensis and P. harinasutai .Parasitol Res 105, 429–439 (2009). https://doi.org/10.1007/s00436-009-1402-4

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• Received: 05 February 2009
• Accepted: 10 March 2009
• Published: 27 March 2009
• Issue date: July 2009
• DOI: https://doi.org/10.1007/s00436-009-1402-4

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