Development of an immunodiagnosis method using recombinant PsCP for detection of Paragonimus skrjabini infection in human (original) (raw)

Abstract

Paragonimiasis skrjabini is a kind of zoonosis and prevalent in 16 provinces in China, such as Chongqing, Fujian, Sichuan, and Yunnan. However, sensitive and efficient diagnostic methods for the infection are limited. In order to provide a more convenient and simple method for serologic diagnosis, the recombinant P. skrjabini cysteine protease (PsCP) was expressed, purified, and then used to develop an indirect enzyme-linked immunosorbent assay (ELISA) for detecting anti-PsCP antibodies in human. Given the positive/negative cutoff value as 0.606, the maximum dilution of human sera in which anti-PsCP antibodies could be detected was 1:12,800. In addition, the coefficients of variation (CVs) of inter-assay and intra-assay experiments were both below 10 %. Furthermore, the sensitivity of the PsCP-based ELISA was 95.5 %, and the indirect ELISA displays no cross-reactivity with human antisera against Echinococcus granulosus, Taenia solium, Schistosoma japonicum, and Trichinella spiralis, either. In conclusion, recombinant PsCP was readily produced and used to establish a simple PsCP-based ELISA that provided a highly specific and sensitive method for analysis of clinical samples. Besides, the method can also probably be used to diagnose P. skrjabini infection in animals.

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Acknowledgments

We thank the volunteers for providing their sera in this study and thank Professor Ya Zhao from Fourth Military Medical University and Professor Yimei Yang from Dali University, China, for their presentation of the human antisera. And thanks to Luhan Wang from Chongqing Foreign Language School for polishing expression in English. This research was supported by the Natural Science Foundation of Chongqing (CSTC, 2007BB5055). The author’s laboratory was supported in part by grants from the National Natural Science Foundation of China (81271875).

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Authors and Affiliations

1. Institute of Tropical Medicine, Third Military Medical University, Chongqing, 400038, China
   Shasha Yu, Guoping Ai, Nan Ye & Ying Wang
2. Department of Pathogen Biology, Third Military Medical University, Chongqing, 400038, China
   Xilin Zhang & Hong Zheng
3. Department of Pathogen Biology, Southwest Medical University, Luzhou, 646000, China
   Wenbi Chen

Authors

1. Shasha Yu
2. Xilin Zhang
3. Wenbi Chen
4. Hong Zheng
5. Guoping Ai
6. Nan Ye
7. Ying Wang

Corresponding author

Correspondence toYing Wang.

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All samples used in this study were anonymized. And consent was obtained from all participants enrolled in this study. This study was approved by the Ethics Committee for Health Research, Third Military Medical University, China

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Yu, S., Zhang, X., Chen, W. et al. Development of an immunodiagnosis method using recombinant PsCP for detection of Paragonimus skrjabini infection in human.Parasitol Res 116, 377–385 (2017). https://doi.org/10.1007/s00436-016-5300-2

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• Received: 26 July 2016
• Accepted: 13 October 2016
• Published: 28 October 2016
• Issue date: January 2017
• DOI: https://doi.org/10.1007/s00436-016-5300-2

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