Involvement of central TRPV1 receptors in pentylenetetrazole and amygdala-induced kindling in male rats (original) (raw)

Abstract

Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel that is involved in modulation of diverse physiological processes. The role of this receptor in epilepsy has not been studied well. Therefore, we investigated the role of central TRPV1 receptors on the development of pentylenetetrazole (PTZ) and amygdala-induced kindling in rats. Male Wistar rats received subconvulsive dose of PTZ intraperitoneally, every other day. TRPV1 receptor agonist, OLDA and its antagonist, AMG-9810 were injected intracerebroventricularly 30 min prior to PTZ administration. In electrical kindling, stimulating and recording electrodes were implanted in the right amygdala of male rats. After kindling, the effect of TRPV1 receptor agonist or antagonist on afterdischarge duration (ADD), latency to the onset of bilateral forelimb clonuses (S4L) and duration of loss of equilibrium (stage 5 seizures, S5D) were measured. The results demonstrated that, OLDA at the doses of 0.01, 0.1 and 1 μg/rat, significantly accelerated the incidence of all seizure stages, increased S5D and decreased S4L in the PTZ model of kindling. Also, in amygdala kindling, S5D and ADD were significantly reduced following the administration of AMG-9810. In contrast, OLDA significantly aggravated the indices of seizure in both models of epileptic seizure. This study demonstrated that central TRPV1 receptors may be involved in the development of electrical and PTZ-induced kindling.

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Authors and Affiliations

  1. Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
    Mohsen Shirazi, Masoud Amin, Mohammad Ebrahim Rezvani & Ali Shamsizadeh
  2. Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
    Mahin Izadi & Mohammad Ebrahim Rezvani
  3. Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
    Ali Roohbakhsh
  4. Iran National Science Foundation, Northern Kargar Street, Next to Jalal Ale Ahmad Intersection, Fifth Alley, No. 33, P.O. Box: 15875/3939, Tehran, Iran
    Ali Shamsizadeh

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  1. Mohsen Shirazi
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  2. Mahin Izadi
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  3. Masoud Amin
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  4. Mohammad Ebrahim Rezvani
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  5. Ali Roohbakhsh
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  6. Ali Shamsizadeh
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Correspondence toMohammad Ebrahim Rezvani.

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Shirazi, M., Izadi, M., Amin, M. et al. Involvement of central TRPV1 receptors in pentylenetetrazole and amygdala-induced kindling in male rats.Neurol Sci 35, 1235–1241 (2014). https://doi.org/10.1007/s10072-014-1689-5

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