Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method (original) (raw)

Abstract

Camellia taliensis is one of the most important wild tea plants in China, especially in Yunnan Province. In this study, we described the development of 15 microsatellite markers from the genome of C. taliensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 24 samples collected from six wild populations of C. taliensis. The average allele number of the microsatellites was four per locus, ranging from 2 to 7. The observed and expected heterozygosities varied from 0.076 to 0.5833 and from 0.1560 to 0.6917, respectively. Cross-species amplification in other three tea plants showed eleven of them holding promise for sister species. These polymorphic SSR markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy, molecular identification and molecular breeding on this tea plant and its allied species and varieties in section Thea genus Camellia.

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References

• Chen L, Yu FL, Tong QQ (2000) Discussions on phylogenetic classification and evolution of section. Thea J Tea Sci 20(1):89–94
  Google Scholar
• Clarke KR, Gorley RN (2001) PRIMER version 5: user manual/tutorial. PRIMER-E Ltd, Plymouth, UK
  Google Scholar
• Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
  Google Scholar
• Hasimoto M, Takasi S (1978) Morphological studies on the origin of the tea plant. A proposal of one place of origin by cluster analysis. Jpn J Trop Agric 21:93–101
  Google Scholar
• Hung CY, Wang KH, Huang CC, Gong X, Ge XJ, Chiang TY (2008) Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet 9:779–781. doi:10.1007/s10592-007-9391-2
  Article CAS Google Scholar
• Kaundun SS, Matsumoto S (2002) Heterologous nuclear and chloroplast microsatellite amplification and variation in tea, Camellia sinensis. Genome 45:1041–1048. doi:10.1139/g02-070
  Article CAS PubMed Google Scholar
• Mondal TK (2002) Assessment of genetic diversity of tea (Camellia sinensis (L.) O. Kuntze) by inter-simple sequence repeat polymerase chain reaction. Euphytica 128:307–315. doi:10.1023/A:1021212419811
  Article Google Scholar
• Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:119–122. doi:10.1007/BF00564200
  Article Google Scholar
• Yeh FC, Yang RC, Boyle TBJ, et al. (1997) POPGENE, the user-friendly freeware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada (program available from: http://www.ualberta.ca/~fyeh/)
• Yu FL (1986) Discussion on the originating place and the originating center of tea plants. J Tea sci 6(1):1–8
  Google Scholar
• Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16. doi:10.1046/j.0962-1083.2001.01418.x
  Article CAS PubMed Google Scholar
• Zhao LP, Liu Z, Chen L, Yao MZ, Wang XC (2008) Generation and characterization of 24 novel EST derived microsatellite from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties. Conserv Genet 9:1327–1331. doi:10.1007/s10592-007-9476-y
  Article CAS Google Scholar

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Acknowledgments

This study was financially supported by National Natural Science Foundation of China (30870169) to Jun Bo Yang.

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Authors and Affiliations

1. SW China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, 650204, Kunming, Yunnan, China
   Jun-Bo Yang, Jing Yang & Hong-Tao Li
2. Jiangxi Agricultural University, Jiangxi, China
   Yu Zhao
3. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 650204, Kunming, Yunnan, China
   Jun-Bo Yang & Shi-Xiong Yang

Authors

1. Jun-Bo Yang
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2. Jing Yang
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3. Hong-Tao Li
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4. Yu Zhao
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5. Shi-Xiong Yang
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Correspondence toShi-Xiong Yang.

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Yang, JB., Yang, J., Li, HT. et al. Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method.Conserv Genet 10, 1621 (2009). https://doi.org/10.1007/s10592-009-9814-3

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• Received: 16 December 2008
• Accepted: 19 January 2009
• Published: 11 February 2009
• DOI: https://doi.org/10.1007/s10592-009-9814-3

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