Inhibition of telomerase limits the growth of human cancer cells (original) (raw)

• Hayflick, L. & Moorhead, P.S. The serial cultivation of human diploid cell strains. Exp. Cell. Res. 25, 585–621 (1961).
  Article CAS Google Scholar
• Shay, J.W., Wright, W.E. & Werbin, H. Defining the molecular mechanisms of human cell immortalization. Biochim. Biophys. Acta 1072, 1– 7 (1991).
  CAS PubMed Google Scholar
• Brown, J.P., Wei, W. & Sedivy, J.M. Bypass of senescence after disruption of p21CIP1/WAF1 gene in normal diploid human fibroblasts. Science 277 , 831–834 (1997).
  Article CAS Google Scholar
• Sager, R. Senescence as a mode of tumor suppression. Environ. Health Perspect. 93, 59–62 ( 1991).
  Article CAS Google Scholar
• Harley, C.B. et al. Telomerase, cell immortality, and cancer. Cold Spring Harb. Symp. Quant. Biol. 59, 307– 315 (1994).
  Article CAS Google Scholar
• Harley, C.B., Futcher, A.B. & Greider, C.W. Telomeres shorten during ageing of human fibroblasts. Nature 345, 458–460 (1990).
  Article CAS Google Scholar
• Hastie, N.D. et al. Telomere reduction in human colorectal carcinoma and with ageing. Nature 346, 866– 868 (1990).
  Article CAS Google Scholar
• Blasco, M.A. et al. Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Cell 91, 25– 34 (1997).
  Article CAS Google Scholar
• Hande, M.P., Samper, E., Lansdorp, P. & Blasco, M.A. Telomere length dynamics and chromosomal instability in cells derived from telomerase null mice. J. Cell. Biol. 144, 589– 601 (1999).
  Article CAS Google Scholar
• Counter, C.M. et al. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11, 1921–1929 ( 1992).
  Article CAS Google Scholar
• Counter, C.M., Hirte, H.W., Bacchetti, S. & Harley, C.B. Telomerase activity in human ovarian carcinoma. Proc. Natl. Acad. Sci. USA 91, 2900–2904 ( 1994).
  Article CAS Google Scholar
• Morales, C.P. et al. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nature Genet. 21, 115–118 (1999).
  Article CAS Google Scholar
• Greider, C.W. in Telomeres (eds. Blackburn, E.H. & Greider, C.W.) 35– 68 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1995).
  Google Scholar
• Feng, J. et al. The RNA component of human telomerase. Science 269, 1236–1241 (1995).
  Article CAS Google Scholar
• Nakamura, T.M. et al. Telomerase catalytic subunit homologs from fission yeast and human. Science 277, 955– 959 (1997).
  Article CAS Google Scholar
• Meyerson, M. et al. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 90, 785–795 ( 1997).
  Article CAS Google Scholar
• Harrington, L. et al. Human telomerase contains evolutionarily conserved catalytic and structural subunits. Genes Dev. 11, 3109–3115 (1997).
  Article CAS Google Scholar
• Kilian, A. et al. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types. Hum. Mol. Genet. 6, 2011–2019 (1997).
  Article CAS Google Scholar
• Nakayama, J. et al. Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinomas. Nature Genet. 18, 65–68 (1998).
  Article CAS Google Scholar
• Kim, N.W. et al. Specific association of human telomerase activity with immortal cells and cancer. Science 266, 2011– 2015 (1994).
  Article CAS Google Scholar
• Ramakrishnan, S., Eppenberger, U., Mueller, H., Shinkai, Y. & Narayanan, R. Expression profile of the putative catalytic subunit of the telomerase gene. Cancer Res. 58, 622–625 (1998).
  CAS PubMed Google Scholar
• Bodnar, A.G. et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279, 349– 352 (1998).
  Article CAS Google Scholar
• Vaziri, H. & Benchimol, S. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr. Biol. 8, 279– 282 (1998).
  Article CAS Google Scholar
• Counter, C.M. et al. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc. Natl. Acad. Sci. USA 95, 14723–14728 ( 1998).
  Article CAS Google Scholar
• Halvorsen, T.L., Leibowitz, G. & Levine, F. Telomerase activity is sufficient to allow transformed cells to escape from crisis. Mol. Cell. Biol. 19, 1864–1870 (1999).
  Article CAS Google Scholar
• Zhu, J., Wang, H., Bishop, J.M. & Blackburn, E.H. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening. Proc. Natl. Acad. Sci. USA 96, 3723–3728 (1999).
  Article CAS Google Scholar
• Hahn, W.C. et al. Creation of human tumor cells with defined genetic elements. Nature 400; 464–468 (1999).
  Article CAS Google Scholar
• Shay, J.W. & Bacchetti, S. A survey of telomerase activity in human cancer. Eur. J. Cancer 33, 787– 791 (1997).
  Article CAS Google Scholar
• Nakamura, T.M. & Cech, T.R. Reversing time: origin of telomerase. Cell 92, 587– 590 (1998).
  Article CAS Google Scholar
• Counter, C.M., Meyerson, M., Eaton, E.N. & Weinberg, R.A. The catalytic subunit of yeast telomerase. Proc. Natl. Acad. Sci. USA 94, 9202–9207 ( 1997).
  Article CAS Google Scholar
• Lingner, J. et al. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276, 561– 567 (1997).
  Article CAS Google Scholar
• Weinrich, S.L. et al. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nature Genet. 17, 498–502 ( 1997).
  Article CAS Google Scholar
• Stewart, N. & Bacchetti, S. Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells. Virology 180, 49–57 (1991).
  Article CAS Google Scholar
• Reddel, R.R., Bryan, T.M. & Murnane, J.P. Immortalized cells with no detectable telomerase activity. A review. Biochemistry (Mosc.) 62, 1254– 1262 (1997).
  CAS Google Scholar
• Counter, C.M., Botelho, F.M., Wang, P., Harley, C.B. & Bacchetti, S. Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes. J. Virol. 68, 3410–3414 (1994).
  CAS PubMed PubMed Central Google Scholar
• Rufer, N., Dragowska, W., Thornbury, G., Roosnek, E. & Lansdorp, P.M. Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry. Nature Biotechnol. 16, 743–747 ( 1998).
  Article CAS Google Scholar
• Goi, K. et al. DNA damage-associated dysregulation of the cell cycle and apoptosis control in cells with germ-line p53 mutation. Cancer Res 57, 1895–1902 (1997).
  CAS PubMed Google Scholar
• Karlseder, J., Broccoli, D., Dai, Y., Hardy, S. & de Lange, T. p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2. Science 283, 1321– 1325 (1999).
  Article CAS Google Scholar
• Strobel, T., Tai, Y.-T., Korsmeyer, S. & Cannistra, S.A. BAD partly reverses paclitaxel resistance in human ovarian carcinoma cells. Oncogene 17, 2419–2427 (1998).
  Article CAS Google Scholar
• Kastan, M.B. et al. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Cell 71, 587–597 (1992).
  Article CAS Google Scholar
• Glukhov, A.I., Zimnik, O.V., Gordeev, S.A. & Severin, S.E. Inhibition of telomerase activity of melanoma cells in vitro by antisense oligonucleotides. Biochem. Biophys. Res. Commun. 248 , 368–371 (1998).
  Article CAS Google Scholar
• Bisoffi, M. et al. Inhibition of human telomerase by a retrovirus expressing telomeric antisense RNA. Eur. J. Cancer 34, 1242–1249 (1998).
  Article CAS Google Scholar
• Kondo, S. et al. Antisense telomerase treatment: induction of two distinct pathways, apoptosis and differentiation. FASEB J. 12 , 801–811 (1998).
  Article CAS Google Scholar
• Norton, J.C., Piatyszek, M.A., Wright, W.E., Shay, J.W. & Corey, D.R. Inhibition of human telomerase activity by peptide nucleic acids. Nature Biotechnol. 14, 615–619 (1996).
  Article CAS Google Scholar
• Lundblad, V. & Blackburn, E.H. An alternative pathway for yeast telomere maintenance rescues est1- senescence. Cell 73, 347–360 (1993).
  Article CAS Google Scholar
• Nakamura, T.M., Cooper, J.P. & Cech, T.R. Two modes of survival of fission yeast without telomerase. Science 282, 493–496 (1998).
  Article CAS Google Scholar
• Levine, A.J. p53, the cellular gatekeeper for growth and division. Cell 88, 323–331 (1997).
  Article CAS Google Scholar
• Aas, T. et al. Specific p53 mutations are associated with de novo resistance to doxorubicin in breast cancer patients. Nature Med. 2, 811–814 (1996).
  Article CAS Google Scholar
• Lowe, S.W., Ruley, H.E., Jacks, T. & Houseman, S.E. p53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 74, 957–968 ( 1993).
  Article CAS Google Scholar
• Chin, L. et al. p53 deficiency rescues the adverse effects of telomere loss and cooperates with telomere dysfunction to accelerate carcinogenesis. Cell 97, 527–538 ( 1999).
  Article CAS Google Scholar
• de Lange, T. et al. Structure and variability of human chromosome ends. Mol. Cell. Biol. 10, 518–527 (1990).
  Article CAS Google Scholar
• Strahl, C. & Blackburn, E.H. Effects of reverse transcriptase inhibitors on telomere length and telomerase activity in two immortalized human cell lines. Mol. Cell. Biol. 16, 53 –65 (1996).
  Article CAS Google Scholar
• Morgenstern, J.P. & Land, H. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 18, 3587–3596 (1990).
  Article CAS Google Scholar
• Kim, N.W. & Wu, F. Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP). Nucleic Acids Res. 25, 2595– 2597 (1997).
  Article CAS Google Scholar
• Seabright, M. A rapid banding technique for human chromosomes. Lancet 2, 971–972 (1971).
  Article CAS Google Scholar
• Landsdorp, P.M. et al. Heterogeneity in telomere length of human chromosomes. Hum. Mol. Genet. 5, 685– 691 (1996).
  Article Google Scholar
• Schmid, I., Uittenbogaart, C.H. & Giorgi, J.V. A gentle fixation and permeabilization method for combined cell surface and intracellular staining with improved precision in DNA quantification. Cytometry 12, 279– 285 (1991).
  Article CAS Google Scholar