Immunization against blood-stage rodent malaria using purified parasite antigens (original) (raw)

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• Published: 26 November 1981

Nature volume 294, pages 361–364 (1981)Cite this article

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Abstract

We have reported the production of several hybridoma lines secreting monoclonal antibodies recognizing antigens of the blood forms of a rodent malaria parasite, _Plasmodium yoelii yoelii_1,2. Line WIC 25.77 secretes an IgG2a (antibody 25.77) which reacts specifically with merozoites in the indirect immunofluorescence (IIF) assay, and which was protective on passive transfer. Another of the hybridoma lines, WIC 25.1, secretes an IgG2a (antibody 25.1) which reacts with an antigen apparently associated with the membranes of schizonts, but this antibody was not protective on passive transfer. We have used our monoclonal antibodies to identify and purify antigens of P. y. yoelii, and we report here that mice immunized with either a 235,000 apparent molecular weight (MW) merozoite-specific protein or a 230,000 MW schizont protein and its proteolytic derivatives were protected against infection with P. y. yoelii.

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Authors and Affiliations

1. Department of Immunochemistry, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent, BR3 3BS, UK
   Anthony A. Holder & Robert R. Freeman

Authors

1. Anthony A. Holder
2. Robert R. Freeman

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Holder, A., Freeman, R. Immunization against blood-stage rodent malaria using purified parasite antigens.Nature 294, 361–364 (1981). https://doi.org/10.1038/294361a0

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• Received: 03 July 1981
• Accepted: 13 October 1981
• Issue date: 26 November 1981
• DOI: https://doi.org/10.1038/294361a0

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