Experimental manipulation of a contact guidance system in amphibian gastrulation by mechanical tension (original) (raw)
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- Published: 02 February 1984
Nature volume 307, pages 453–455 (1984)Cite this article
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Abstract
Contact guidance1,2 has been implied in various morphogenetic movements including neural crest cell migration3, primordial germ cell migration4 and guidance of axonal growth cone5. In urodele gastrulae, we reported6 the presence of an aligned network of extracellular fibrils on the inside of the ectodermal layer and suggested that it guides the migration of the presumptive mesodermal cells from the blastopore towards the animal pole. We also reported7 in vitro experiments in which the fibril network of the ectodermal layer was transferred onto the surface of a coverslip. Dissociated mesodermal cells attach to and locomote actively on such conditioned surfaces in an oriented fashion along the blastopore–animal pole axis (bp–ap axis) of the ectodermal layer that conditioned the surface. Recent reports8,18 suggest that these fibrils contain fibronectin. We now report that the fibril network on the conditioned surface can be artificially aligned in any orientation by exerting mechanical tension on the ectodermal layer during the conditioning. Such prepared surfaces cause cell movements aligned along the tension axis, even when the tension axis is perpendicular to the natural axis of alignment along the bp–ap axis. These results suggest that the extracellular matrix fibrils aligned by the mechanical stress that arises in embryos during development can orient cell migration by the contact guidance, in a similar manner to that reported in the collagen gel and fibroblasts system9,10.
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References
- Weiss, P. J. exp. Zool. 100, 353–386 (1945).
Article CAS Google Scholar - Dunn, G. A. in Cell Behaviour, 247–280 (Cambridge University Press, 1982).
Google Scholar - Löfberg, J., Ahlfors, K. & Fällström, C. Devi Biol. 75, 148–167 (1980).
Article Google Scholar - Heasman, J., Hynes, R. O., Swan, A. P., Thomas, V. & Wylie, C. C. Cell 27, 437–447 (1981).
Article CAS Google Scholar - Silver, J. & Ogawa, M. Y. Science 220, 1067–1069 (1983).
Article ADS CAS Google Scholar - Nakatsuji, N., Gould, A. C. & Johnson, K. E. J. Cell Sci. 56, 207–222 (1982).
CAS PubMed Google Scholar - Nakatsuji, N. & Johnson, K. E. J. Cell Sci. 59, 43–60 (1983).
CAS PubMed Google Scholar - Boucaut, J.-C. & Darribere, T. Cell Differentiation 12, 77–83 (1983).
Article CAS Google Scholar - Harris, A. K., Stopak, D. & Wild, P. Nature 290, 249–251 (1981).
Article ADS CAS Google Scholar - Stopak, D. & Harris, A. K. Devl Biol. 90, 383–398 (1982).
Article CAS Google Scholar - Rugh, R. Experimental Embryology 3rd edn (Burgess, Minneapolis, 1962).
Google Scholar - Keller, R. E. J. Embryol. exp. Morph. 60, 201–234 (1980).
CAS PubMed Google Scholar - Johnson, K. E. Exp. Cell Res. 101, 71–77 (1976).
Article CAS Google Scholar - Kubota, H. Y. & Durston, A. J. J. Embryol. exp. Morph. 44, 71–80 (1978).
CAS PubMed Google Scholar - Nakatsuji, N. & Johnson, K. E. Cell Motility 2, 149–161 (1982).
Article CAS Google Scholar - Stearns, R. N. & Kostellow, A. B. in The Chemical Basis of Development, 448–457 (Johns Hopkins University Press, Baltimore, 1958).
Google Scholar - Feldman, M. J. Embryol. exp. Morph. 3, 251–255 (1955).
Google Scholar - Boucaut, J. C. & Darribere, T. Cell Tissue Res. 234, 135–146 (1983).
Article CAS Google Scholar
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Author notes
- Norio Nakatsuji
Present address: MRC Mammalian Development Unit, University College London, Wolfson House, 4 Stephenson Way, London, NW1 2HE, UK
Authors and Affiliations
- Department of Anatomy, The George Washington University Medical Center, Washington DC, 20037, USA
Norio Nakatsuji & Kurt E. Johnson
Authors
- Norio Nakatsuji
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Nakatsuji, N., Johnson, K. Experimental manipulation of a contact guidance system in amphibian gastrulation by mechanical tension.Nature 307, 453–455 (1984). https://doi.org/10.1038/307453a0
- Received: 12 September 1983
- Accepted: 25 November 1983
- Issue Date: 02 February 1984
- DOI: https://doi.org/10.1038/307453a0
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