Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes (original) (raw)
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- Published: 10 January 1985
Nature volume 313, pages 141–143 (1985)Cite this article
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Abstract
The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators1–3 demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), and that the product of this reaction, inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), could cause a release of non-mitochondrial calcium. These findings have been verified in other systems4–6. Although the relationship between the hydrolysis of PtdIns(4,5)P2 and the mobilization of intracellular calcium was clearly demonstrated, the direct link between Ins(1,4,5)P3 production and the physiological response was only implied. We have investigated the possibility that the intracellular release of Ins(1,4,5)P3 mediates the muscarinic–cholinergic response is Xenopus oocytes, and we show here that intracellularly injected Ins(1,4,5)P3 mimics the muscarinic depolarizing chloride current in Xenopus oocytes. This is the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response.
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References
- Berridge, M. J. Biochem. J. 212, 849–858 (1983).
Article CAS Google Scholar - Berridge, M. J., Dawson, R. M. C., Downes, P. C., Heslop, J. P. & Irvine, R. F. Biochem. J. 212, 473–482 (1983).
Article CAS Google Scholar - Streb, H., Irvine, R. F., Berridge, M. J. & Schulz, I. Nature 306, 67–69 (1983).
Article ADS CAS Google Scholar - Agranoff, B. W., Murthy, P. & Seguin, E. B. J. biol. Chem. 258, 2076–2078 (1983).
CAS Google Scholar - Putney, J. W. Jr, Burgess, G. M., Halenda, S. P., McKinney, J. S. & Rubin, R. P. Biochem. J. 212, 483–488 (1983).
Article CAS Google Scholar - Poggioli, J., Weiss, S. J., McKinney, J. S. & Putney, J. W. Jr. Molec. Pharmac. 23, 71–77 (1983).
CAS Google Scholar - Kusano, K., Miledi, R. & Stinnakre, J. Nature 270, 739–741 (1977).
Article ADS CAS Google Scholar - Dascal, N. & Landau, E. M. Life Sci. 27, 1423–1428 (1980).
Article CAS Google Scholar - Dascal, N., Landau, E. M. & Lass, Y. J. Physiol., Lond. 352, 551–574 (1984).
Article CAS Google Scholar - Dascal, N. & Landau, E. M. Proc. natn. Acad. Sci. U.S.A. 79, 3052–3056 (1982).
Article ADS CAS Google Scholar - Robbins, N. & Molenaar, P. C. Proc. R. Soc. B213, 59–72 (1981).
ADS CAS Google Scholar - Soreq, H., Parvari, R. & Silman, I. Proc. natn. Acad. Sci. U.S.A. 79, 830–834 (1982).
Article ADS CAS Google Scholar - Dascal, N., Yekuel, R. & Oron, Y. J. exp. Zool. 230, 131–135 (1984).
Article CAS Google Scholar - Downes, P. C. & Michell, R. H. Biochem. J. 198, 133–140 (1981).
Article CAS Google Scholar - Downes, P. C., Mussat, M. C. & Michell, R. H. Biochem. J. 203, 169–177 (1982).
Article CAS Google Scholar - Bartlett, G. R. J. biol. Chem. 234, 466–468 (1969).
Google Scholar - Fein, A., Payne, R., Corson, D. W., Berridge, M. J. & Irvine, R. F. Nature 311, 157–160 (1984).
Article ADS CAS Google Scholar - Brown, J. E. et al. Nature 311, 161–163 (1984).
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Authors and Affiliations
- Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel
Y. Oron, N. Dascal, E. Nadler & M. Lupu
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- Y. Oron
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Oron, Y., Dascal, N., Nadler, E. et al. Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.Nature 313, 141–143 (1985). https://doi.org/10.1038/313141a0
- Received: 14 June 1984
- Accepted: 08 October 1984
- Issue Date: 10 January 1985
- DOI: https://doi.org/10.1038/313141a0