Voltage-dependent potassium currents in cultured astrocytes (original) (raw)

Nature volume 315, pages 229–232 (1985)Cite this article

Abstract

Astrocytes are a major cell type in the mammalian central nervous system (CNS), yet their functions remain uncertain. There are two principal classes of these glial cells—protoplasmic astrocytes, found mainly in grey matter, and fibrous astrocytes, which occur mainly in white matter1. Recently, these two types of astrocytes have been distinguished in cultures of developing CNS and have been shown to be biochemically distinct2. Because both types contain large numbers of glial filaments in vitro and hence appear ‘fibrous’, we will refer to them as type 1 (protoplasmic) and type 2 (fibrous) astrocytes2. Most type 2 astrocytes in culture share several properties with neurones2; for example, they have a process-bearing morphology and bind tetanus toxin and the monoclonal antibody A2B5 (ref. 3), both of which recognize specific gangliosides3,4 and were initially considered to be neurone-specific markers in the CNS3,5,6. We have therefore investigated whether type 2 astrocytes also share electrophysiological properties with neurones. Using intracellular microelectrode and ‘whole-cell’ (patch-clamp) recording techniques, we have now found that both type 1 and type 2 astrocytes in culture have time- and voltage-dependent potassium ion conductances which, until recently, were considered to be confined largely to electrically excitable cells.

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References

  1. Peters, A., Palay, S. L. & Webster, H. de F. The Fine Structure of the Nervous System: The Neurons and Supporting Cells 2nd edn, 233–244 (Saunders, Philadelphia, 1976).
    Google Scholar
  2. Raff, M. C., Abney, E. R., Cohen, J., Lindsay, R. & Noble, M. J. Neurosci. 3, 1289–1300 (1983).
    Article CAS Google Scholar
  3. Eisenbarth, G. S., Walsh, F. S. & Nirenberg, M. Proc. natn. Acad. Sci. U.S.A 76, 4913–4917 (1979).
    Article CAS ADS Google Scholar
  4. Van Heyningen, W. E. J. gen. Microbiol. 31, 375–387 (1963).
    Article CAS Google Scholar
  5. Dimpfel, W., Neale, J. H. & Habermann, E. Naunyn-Schmiedebergs Archs Pharmak. 290, 329–333 (1975).
    Article CAS Google Scholar
  6. Mirsky, R., Wendon, L. M. B., Black, P., Stolkin, C. & Bray, D. Brain Res. 148, 251–259 (1978).
    Article CAS Google Scholar
  7. Raff, M. C., Williams, B. P. A. & Miller, R. H. EMBO J. 3, 1857–1864 (1984).
    Article CAS Google Scholar
  8. Raff, M. C., Miller, R. H. & Noble, M. Nature 303, 390–396 (1983).
    Article CAS ADS Google Scholar
  9. Raff, M. C. et al. Nature 274, 813–816 (1978).
    Article CAS ADS Google Scholar
  10. Adelman, W. J. & Senft, J. P. J. gen. Physiol. 50, 279–293 (1966).
    Article Google Scholar
  11. Benzanilla, F. & Armstrong, C. M. J. gen. Physiol. 60, 588–608 (1972).
    Article Google Scholar
  12. Marty, A. & Neher, E. in Single Channel Recording (eds Sakmann, B. & Neher, E.) 107–122 (Plenum, New York, 1983).
    Book Google Scholar
  13. McCarthy, K. D. & de Vellis, J. J. Cell Biol. 85, 890–902 (1980).
    Article CAS Google Scholar
  14. Kettenmann, H., Orkand, R. K. & Schachner, M. J. Neurosci. 3, 506–516 (1983).
    Article CAS Google Scholar
  15. Kettenmann, H. et al. Pflügers Arch. ges. Physiol. 401, 324–332 (1984).
    Article CAS Google Scholar
  16. Armstrong, C. M. & Hille, B. J. gen. Physiol. 59, 388–400 (1972).
    Article CAS Google Scholar
  17. Stallcup, W., Levine, J. & Rashke, W. Cold Spring Harb. Rep. Neurosci. 2, 39–49 (1981).
    Google Scholar
  18. Bowman, C. L., Kimelberg, H. K., Frangakis, M. V., Berwald-Netter, Y. & Edwards, C. J. Neurosci. 4, 1527–1534 (1984).
    Article CAS Google Scholar
  19. Bevan, S., Chiu, S. Y., Gray, P. T. A. & Ritchie, J. M. J. Physiol., Lond. (in the press).
  20. MacVicar, B. A. Science 226, 1345–1347 (1984).
    Article CAS ADS Google Scholar

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  1. Zoology Department, University College London, Gower Street, London, WC1E 6BT, UK
    Stuart Bevan & Martin Raff

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  1. Stuart Bevan
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  2. Martin Raff
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Bevan, S., Raff, M. Voltage-dependent potassium currents in cultured astrocytes.Nature 315, 229–232 (1985). https://doi.org/10.1038/315229a0

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