No functional γ-chain transcripts detected in an alloreactive cytotoxic T-cell clone (original) (raw)

Nature volume 321, pages 876–878 (1986) Cite this article

Abstract

Three groups of genes that undergo rearrangements during T-cell maturation have been isolated from T cells1–13. Two of them encode the _α_- and _β_-subunits of the T-cell antigen receptor and are shared between antigen-specific, major histocompatibility (MHC) class I-restricted cytotoxic T cells and antigen-specific, MHC class II-restricted helper T cells1–10,14. The third group of genes, called γ, is preferentially transcribed in cytotoxic T cells12,15. This led to the hypothesis that the unidentified _γ_-gene products could be part of a putative T-cell receptor responsible for MHC class I recognition16,17. We report here on the isolation of three different types of _γ_-gene transcripts of an alloreactive cytotoxic T-cell clone (3F9). Two are derived from two rearrangements that have occurred at the same locus (V γ 10.8A to J γ 10.5 and transcribed with C _γ_10.5), while the third involves a new V _γ_-gene segment that is joined to J _γ_13.4 and transcribed with C _γ_13.4. All these rearrangements are abortive and lead to the formation of non-functional _γ_-chain genes because the proper translational reading frame is not maintained. Because the second copy of the C _γ_13.4 gene segment is deleted and as C _γ_7.5 is considered to be a pseudogene and has not undergone any rearrangements in 3F9, we conclude that the alloreactive cytotoxic T-cell clone 3F9 does not contain a functional transcript of a known _γ_-chain gene.

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Authors and Affiliations

  1. Institut für Pathologie der Universität Zürich, Universitätsspital, CH-8091, Zürich, Switzerland
    F. Rupp, G. Frech, H. Hengartner, R. M. Zinkernagel & R. Joho

Authors

  1. F. Rupp
  2. G. Frech
  3. H. Hengartner
  4. R. M. Zinkernagel
  5. R. Joho

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Rupp, F., Frech, G., Hengartner, H. et al. No functional _γ_-chain transcripts detected in an alloreactive cytotoxic T-cell clone.Nature 321, 876–878 (1986). https://doi.org/10.1038/321876a0

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