Identification of the long ubiquitin extension as ribosomal protein S27a (original) (raw)

Nature volume 338, pages 438–440 (1989)Cite this article

Abstract

Two proteins of unknown function are encoded by 3' in-frame extensions of ubiquitin genes1. The polypeptides are synthesized as an additional 52 (refs 2–4) or 76–80 (refs 4,5) amino acids on the C terminus of ubiquitin, an unusual arrangement conserved in man3,5, yeast4 and plants (J. Callis and R. Vierstra, personal communication). Although not homologous to each other or to ubiquitin, both extension proteins are highly basic and contain patterns of cysteine and histidine similar to those proposed to form 'zinc fingers'6,7. The longer C-terminal extension protein (CEP80) is 30% lysine and arginine and, when denatured, behaves like a small cationic protein8. Its properties after isolation in physiological conditions, however, suggested that CEP80 is part of an RNA-protein complex. Using the antibodies that confirmed the presence of CEP80 in eukaryotic cells8, we show here that the protein is located on ribosomes. Immunoblotting of rat 40S subunit proteins specifically identifies CEP80 as ribosomal protein S27a.

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References

  1. Schlesinger, M. J. & Bond, U. Oxf. Surv. Euk. Genes 4, 77 (1987).
    CAS Google Scholar
  2. Müller-Taubenberger, A., Westphal, M., Jaeger, E., Noegel, A. & Gerisch, G. FEBS Lett. 229, 273–278 (1988).
    Article PubMed Google Scholar
  3. Salvensen, G., Lloyd, C. & Farley, D. Nucleic Acids Res. 15, 5485 (1987).
    Article Google Scholar
  4. Özkaynak, E., Finley, D., Solomon, M. J. & Varshavsky, A. EMBO J. 6, 1429–1439 (1987).
    Article PubMed PubMed Central Google Scholar
  5. Lund, P. K. et al. J. biol. Chem. 260, 7609–7613 (1985).
    CAS PubMed Google Scholar
  6. Berg, J. M. Science 232, 485–487 (1986).
    Article ADS CAS PubMed Google Scholar
  7. Evans, R. M. & Hollenberg, S. M. Cell 52, 1–3 (1988).
    Article CAS PubMed Google Scholar
  8. Redman, K. L. & Rechsteiner, M. J. biol. Chem. 263, 4926–4931 (1988).
    CAS PubMed Google Scholar
  9. Collatz, E., Wool, I. G., Lin, A. & Stöffler, G. J. biol. Chem. 251, 4666–4672 (1976).
    CAS PubMed Google Scholar
  10. Collatz, E. et al. J. biol. Chem. 252, 9071–9080 (1977).
    CAS PubMed Google Scholar
  11. Odani, S., Kenmochi, N. & Ogata, K. J. Biochemistry 103, 872–877 (1988).
    Article CAS Google Scholar
  12. Lin, A., Chan, Y.-L., Jones, R. & Wool, I. G. J. biol. Chem. 262, 14343–14351 (1987).
    CAS PubMed Google Scholar
  13. Cornish-Bowden, A. Analyt. Biochem. 105, 233–238 (1980).
    Article CAS PubMed Google Scholar
  14. McConkey, E. H. et al. Molec. gen. Genet. 169, 1–6 (1979).
    Article CAS PubMed Google Scholar
  15. Madjar, J.-J., Arpin, M., Buisson, M. & Reboud, J.-P. Molec. gen. Genet. 171, 121–134 (1979).
    Article CAS PubMed Google Scholar
  16. Hardy, S. J. S. & Kurland, C. G. Biochemistry 5, 3676–3684 (1966).
    Article CAS PubMed Google Scholar
  17. Nue, H. C. & Heppel, L. A. Proc. natn. Acad. Sci. U.S.A. 51, 1267–1274 (1964).
    Article ADS Google Scholar
  18. Finley, D. et al. in Ubiquitin (ed M. Rechsteiner) 39–75 (Plenum, New York), (1988).
    Book Google Scholar
  19. Finley, D., Bartel, B. & Varshavsky, A. Nature (in the press).
  20. Otaka, E., Higo, K. & Osawa, S. Biochemistry 21, 4545–4550 (1982).
    Article CAS PubMed Google Scholar
  21. Otaka, E. & Osawa, S. Molec. gen. Genet. 181, 176–182 (1981).
    Article CAS Google Scholar
  22. Rechsteiner, M. A. Rev. Cell Biol. 3, 1–30 (1987).
    Article CAS Google Scholar
  23. Nomura, M., Gourse, R. & Baughman, G. A. Rev. Biochem. 53, 75–117 (1984).
    Article CAS Google Scholar
  24. Brown, G. E., Kolb, A. J. & Stanley, W. M. Meth. Enzym 30, 368–387 (1974).
    Article CAS PubMed Google Scholar
  25. Salinovich, O. & Montelaro, R. C. Analyt. Biochem. 156, 341–347 (1986).
    Article CAS PubMed Google Scholar
  26. Madjar, J.-J., Michel, S., Cozzone, A. J. & Reboud, J.-P. Analyt. Biochem. 92, 174–182 (1979).
    Article CAS PubMed Google Scholar
  27. Fling, S. P. & Gregerson, D. S. Analyt. Biochem. 155, 83–88 (1986).
    Article CAS PubMed Google Scholar

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  1. Kent L. Redman
    Present address: Department of Biology, Molecular and Cellular Biology Section, University of Alabama, Box 870344, Tuscaloosa, Alabama, 35487–0344, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, 84132, USA
    Kent L. Redman & Martin Rechsteiner

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  1. Kent L. Redman
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  2. Martin Rechsteiner
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Redman, K., Rechsteiner, M. Identification of the long ubiquitin extension as ribosomal protein S27a.Nature 338, 438–440 (1989). https://doi.org/10.1038/338438a0

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