Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome (original) (raw)
- Letter
- Published: 23 May 1991
- Alan Ashworth1 na1,
- Graham F. Kay1,
- Penny Cooper1,
- Sandy Smith1,
- Veronica M. McCabe1,
- Dominic P. Norris1,
- Graeme D. Penny1,
- Dipika Patel1 &
- …
- Sohaila Rastan1 na2
Nature volume 351, pages 329–331 (1991)Cite this article
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Abstract
X-CHROMOSOME inactivation in mammals is a regulatory phenomenon whereby one of the two X chromosomes in female cells is genetically inactivated, resulting in dosage compensation for X-linked genes between males and females1. In both man and mouse, X-chromosome inactivation is thought to proceed from a single _cis_-acting switch region or inactivation centre (XlC/Xic)2–5. In the human, XIC has been mapped to band Xql3 (ref. 6) and in the mouse to band XD (ref. 7), and comparative mapping has shown that the XIC regions in the two species are syntenic8. The recently described human XIST gene maps to the XIC region6 and seems to be expressed only from the inactive X chromosome9. We report here that the mouse Xist gene maps to the Xic region of the mouse X chromosome and, using an interspecific Mus spretus/Mus musculus domesticus F1 hybrid mouse carrying the T(X; 16)16H translocation, show that Xist is exclusively expressed from the inactive X chromosome. Conservation between man and mouse of chromosomal position and unique expression exclusively from the inactive X chromosome lends support to the hypothesis that XIST and its mouse homologue are involved in X-chromosome inactivation.
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Author notes
- Alan Ashworth: Chester Beatty Laboratories, The Institute of Cancer Research, London SW3 6JB, UK
- Sohaila Rastan: To whom correspondence should be addressed
Authors and Affiliations
- Section of Comparative Biology, MRC Clinical Research Centre, Harrow, HA1 3UJ, UK
Neil Brockdorff, Alan Ashworth, Graham F. Kay, Penny Cooper, Sandy Smith, Veronica M. McCabe, Dominic P. Norris, Graeme D. Penny, Dipika Patel & Sohaila Rastan
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Brockdorff, N., Ashworth, A., Kay, G. et al. Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome.Nature 351, 329–331 (1991). https://doi.org/10.1038/351329a0
- Received: 13 February 1991
- Accepted: 23 April 1991
- Issue Date: 23 May 1991
- DOI: https://doi.org/10.1038/351329a0