Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1 (original) (raw)
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- Published: 07 November 1991
Nature volume 354, pages 80–82 (1991)Cite this article
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Abstract
THE product of the gene RCC1 (regulator of chromosome condensation) in a BHK cell line is involved in the control of mitotic events1. Homologous genes have been found in _Xenopus_2, Drosophila_3 and yeast4,5. A human genomic DNA fragment and complementary DNA that complement a temperature-sensitive mutation of_RCC1 in BHK21 cells6,7 encode a protein of relative molecular mass 45,000 (_M_r45K) which is located in the nucleus and binds to chromatin8. We have recently isolated a protein from HeLa cells that strongly binds an anti-RCCl antibody and has the same molecular mass, DNA-binding properties, and amino-acid sequence as the 205 residues already identified9. HeLa cell RCCl is complexed to a protein of M_r 25K (ref. 9). We have shown10 that this 25K protein has a sequence homologous to the translated reading frame of_TC4, a cDNA found by screening a human teratocarcinoma cDNA library with oligonucleotides coding for a ras consensus sequence11, and that the protein binds GDP and GTP. We have referred to this protein as the Ran protein (ras_-related nuclear protein). In addition to the fraction of Ran protein complexed to RCCl, a 25-fold molar excess of the protein over RCCl was found in the nucleoplasm of HeLa cells. Here we show that RCCl specifically catalyses the exchange of guanine nucleo-tides on the Ran protein but not on the protein c-Ha-ras p21 (p21_ras).
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Author notes
- Herwig Ponstingl: To whom correspondence should be addressed.
Authors and Affiliations
- Project Molecular Biology of Mitosis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, W-6900, Heidelberg, Germany
F. Ralf Bischoff & Herwig Ponstingl
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- F. Ralf Bischoff
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Bischoff , F., Ponstingl, H. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1.Nature 354, 80–82 (1991). https://doi.org/10.1038/354080a0
- Received: 07 June 1991
- Accepted: 10 September 1991
- Issue Date: 07 November 1991
- DOI: https://doi.org/10.1038/354080a0