Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1 (original) (raw)

Nature volume 354, pages 80–82 (1991)Cite this article

Abstract

THE product of the gene RCC1 (regulator of chromosome condensation) in a BHK cell line is involved in the control of mitotic events1. Homologous genes have been found in _Xenopus_2, Drosophila_3 and yeast4,5. A human genomic DNA fragment and complementary DNA that complement a temperature-sensitive mutation of_RCC1 in BHK21 cells6,7 encode a protein of relative molecular mass 45,000 (_M_r45K) which is located in the nucleus and binds to chromatin8. We have recently isolated a protein from HeLa cells that strongly binds an anti-RCCl antibody and has the same molecular mass, DNA-binding properties, and amino-acid sequence as the 205 residues already identified9. HeLa cell RCCl is complexed to a protein of M_r 25K (ref. 9). We have shown10 that this 25K protein has a sequence homologous to the translated reading frame of_TC4, a cDNA found by screening a human teratocarcinoma cDNA library with oligonucleotides coding for a ras consensus sequence11, and that the protein binds GDP and GTP. We have referred to this protein as the Ran protein (ras_-related nuclear protein). In addition to the fraction of Ran protein complexed to RCCl, a 25-fold molar excess of the protein over RCCl was found in the nucleoplasm of HeLa cells. Here we show that RCCl specifically catalyses the exchange of guanine nucleo-tides on the Ran protein but not on the protein c-Ha-ras p21 (p21_ras).

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  1. Herwig Ponstingl: To whom correspondence should be addressed.

Authors and Affiliations

  1. Project Molecular Biology of Mitosis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, W-6900, Heidelberg, Germany
    F. Ralf Bischoff & Herwig Ponstingl

Authors

  1. F. Ralf Bischoff
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  2. Herwig Ponstingl
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Bischoff , F., Ponstingl, H. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1.Nature 354, 80–82 (1991). https://doi.org/10.1038/354080a0

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