SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast (original) (raw)

Nature volume 357, pages 505–508 (1992)Cite this article

Abstract

IN budding yeast many genes are expressed under cell-cycle control in late Gl. These include a large group of DNA synthesis genes1, the HO gene2 involved in mating-type switching, CTS1 (chitinase)3 and also CLN1 and CLN2 (ref. 4) encoding Gl cyclins. Two factors, encoded by the SWI4 and SWI6_genes, are required for HO (ref. 5), CLN (refs 6, 7) and_CTS1 (ref. 3) gene expression and, at least in the HO promoter, bind to CACGA4 upstream sequences5,7–9(CCBs). This motif is not found upstream of the DNA synthesis genes, which instead have a hexamer element, ACGCGT1 (MCB), an Mlu_I restriction site, that is recognized by a cell-cycle regulated transcription complex DSC1 (ref. 1). This Mlu I-activation system consisting of the MCBs and DSC1 is conserved in fission yeast where a DSCl-like complex controls the cdc22+ribonucleotide reductase gene_10. The Schizosae-charomyces pombe cdc10+ gene encodes a component of DSC1 (ref. 10) and, significantly, this has homology with both the Swi4and Swi6 proteins 8,11. Here we show that Swi6 is an essential component of DSC1 and that deletion of SWI6 impairs the cell-cycle regulation of the DNA synthesis genes, as well as CLN1 and CLN2. Thus Swi6 is the common factor in regulation of all the above genes and may therefore be responsible for the timing of their expression in late Gl.

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Author notes

  1. Leland H. Johnston: To whom correspondence should be addressed.

Authors and Affiliations

  1. Laboratory of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Noel F. Lowndes, Anthony L. Johnson & Leland H. Johnston
  2. Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104, USA
    Linda Breeden

Authors

  1. Noel F. Lowndes
  2. Anthony L. Johnson
  3. Linda Breeden
  4. Leland H. Johnston

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Lowndes, N., Johnson, A., Breeden, L. et al. SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast.Nature 357, 505–508 (1992). https://doi.org/10.1038/357505a0

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