Ypt1p implicated in v-SNARE activation (original) (raw)
- Letter
- Published: 15 December 1994
Nature volume 372, pages 698–701 (1994)Cite this article
- 203 Accesses
- 175 Citations
- 3 Altmetric
- Metrics details
Abstract
SYNAPTOBREVIN-LIKE membrane proteins that reside on transport vesicles, called the vesicle SNARE (v-SNARE), play a key role in ensuring that a vesicle targets and fuses with its correct acceptor compartment1–3. Here we show that Boslp, the v-SNARE of yeast endoplasmic reticulum-to-Golgi transport vesicles, pairs with another integral membrane protein of similar topology (Sec22p) on vesicles. This pairing, which appears to require functional Yptlp (Rab in mammalian cells), may aid the activity of Boslp on this compartment. These findings suggest that Rabs regulate the specificity of membrane fusion by selectively activating the v-SNARE on carrier vesicles. Because the v-SNARE resides on more than one membrane, such a regulated activation step may be necessary to prevent the premature fusion of donor and acceptor compartments4.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Söllner, T. et al. Nature 362, 318–324 (1993).
Article ADS Google Scholar - Warren, G. Nature 362, 297–298 (1993).
Article ADS CAS Google Scholar - Ferro-Novick, S. & Jahn, R. Nature 370, 191–193 (1994).
Article ADS CAS Google Scholar - Novick, P. & Brennwald, P. Cell 75, 597–601 (1993).
Article CAS Google Scholar - Lian, J. P. & Ferro-Novick, S. Cell 73, 735–745 (1993).
Article CAS Google Scholar - Newman, A. P., Shim, J. & Ferro-Novick, S. Molec. cell. Biol. 10, 3405–3414 (1990).
Article CAS Google Scholar - Dascher, C., Ossig, R., Gallwitz, D. & Schmitt, H. D. Molec. cell. Biol. 11, 872–885 (1991).
Article CAS Google Scholar - Ferro-Novick, S. & Novick, P. A. Rev. Cell. Biol 9, 575–599 (1993).
Article CAS Google Scholar - Novick, P., Field, C. & Schekman, R. Cell. 21, 205–215 (1980).
Article CAS Google Scholar - Kaiser, C. A. & Schekman, R. Cell 61, 723–733 (1990).
Article CAS Google Scholar - Shim, J., Newman, A. P. & Ferro-Novick, S. J. Cell. Biol. 113, 55–64 (1991).
Article CAS Google Scholar - Schmitt, H. D., Puzicha, M. & Gallwitz, D. Cell 53, 635–647 (1988).
Article CAS Google Scholar - Rexach, M., Latterich, M. & Schekman, R. J. Cell Biol. 126, 1133–1148 (1994).
Article CAS Google Scholar - Hardwick, K. G. & Pelham, H. R. B. J. Cell Biol. 119, 513–521 (1992).
Article CAS Google Scholar - Søgaard, M. et al. Cell 78, 937–948 (1994).
Article Google Scholar - Newman, A. P., Groesch, M. & Ferro-Novick, S. EMBO J. 12, 3609–3617 (1992).
Article Google Scholar - Hosobuchi, M., Kreis, T. & Schekman, R. Nature 360, 603–605 (1992).
Article ADS CAS Google Scholar - Ruohola, H., Kabcenell, A. K. & Ferro-Novick, S. J. Cell Biol. 107, 1465–1476 (1988).
Article CAS Google Scholar - Groesch, M., Ruohola, H., Bacon, R., Rossi, G. & Ferro-Novick, S. J. Cell Biol. 111, 45–53 (1990).
Article CAS Google Scholar - Groesch, M. E., Rossi, G. & Ferro-Novick, S. Meth. Enzym. 219, 137–152 (1992).
Article CAS Google Scholar - Wilson, D. W., Whiteheart, S. W., Wiedmann, M., Brunner, M. & Rothman, J. E. J. Cell Biol. 117, 531–538 (1992).
Article CAS Google Scholar
Author information
Authors and Affiliations
- Department of Cell Biology and Howard Hughes Medical Institute, Yale University Medical School, New Haven, Connecticut, 06510, USA
Jian P. Lian, Shelly Stone, Yu Jiang, Patrick Lyons & Susan Ferro-Novick
Authors
- Jian P. Lian
You can also search for this author inPubMed Google Scholar - Shelly Stone
You can also search for this author inPubMed Google Scholar - Yu Jiang
You can also search for this author inPubMed Google Scholar - Patrick Lyons
You can also search for this author inPubMed Google Scholar - Susan Ferro-Novick
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Lian, J., Stone, S., Jiang, Y. et al. Ypt1p implicated in v-SNARE activation.Nature 372, 698–701 (1994). https://doi.org/10.1038/372698a0
- Received: 12 July 1994
- Accepted: 02 November 1994
- Issue Date: 15 December 1994
- DOI: https://doi.org/10.1038/372698a0
This article is cited by
Sorting GPI-anchored proteins
- Satyajit Mayor
- Howard Riezman
Nature Reviews Molecular Cell Biology (2004)
Topological restriction of SNARE-dependent membrane fusion
- Francesco Parlati
- James A. McNew
- James E. Rothman
Nature (2000)
Transport-vesicle targeting: tethers before SNAREs
- Suzanne R. Pfeffer
Nature Cell Biology (1999)
- Suzanne R. Pfeffer
The Rab5 effector EEA1 is a core component of endosome docking
- Savvas Christoforidis
- Heidi M. McBride
- Marino Zerial
Nature (1999)
Tethering molecules in membrane traffic
- Brite Sönnichsen
Protoplasma (1999)
- Brite Sönnichsen