A P2X purinoceptor expressed by a subset of sensory neurons (original) (raw)

Nature volume 377, pages 428–431 (1995)Cite this article

Abstract

ATP is known to depolarize sensory neurons, and may play a role in nociceptor activation when released from damaged tissue1-10. Here we report the molecular cloning and characterization of a new member of the P2X receptor family3,11,12, P2X3, expressed by these cells. The channel transcript was present in a subset of rat dorsal-root-ganglion sensory neurons, some of which express nociceptor-associated markers; it was absent in other tissues that were tested, including sympathetic, enteric and central nervous system neurons. Moreover, when expressed in Xenopus oocytes, the channel showed an ATP-dependent cation flux. P2X3 is the only ligand-gated channel known to be expressed exclusively by a subset of sensory neurons. The remarkable selectivity of expression of the channel coupled with its sensory neuron-like pharmacology suggests that this channel may transduce ATP-evoked nociceptor activation.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Burnstock, G. Drug Dev. Res. 28, 195–206 (1993).
    Article CAS Google Scholar
  2. Khakh, B. S., Humphrey, P. P. A. & Surprenant, A. J. Physiol., Lond. 484, 385–395 (1995).
    Article CAS Google Scholar
  3. Abbracchio, M. P. & Burnstock, G. Pharmac. Ther. 64, 445–475 (1994).
    Article CAS Google Scholar
  4. Krishtal, O. A. & Marchenko, S. M. Biomed. Res. 7, 79–81 (1986).
    Article CAS Google Scholar
  5. Krishtal, O. A., Marchenko, S. M., Obukhov, A. G. & Volkova, T. M. Br. J. Pharmac. 95, 1057–1062 (1988).
    Article CAS Google Scholar
  6. Jahr, C. E. & Jessell, T. M. Nature 304, 730–733 (1983).
    Article ADS CAS Google Scholar
  7. Bean, B. P. J. Neurosci. 10, 1–10 (1990).
    Article CAS Google Scholar
  8. Bean, B. P., Williams, C. A. & Ceelen, P. W. J. Neurosci. 10, 11–19 (1990).
    Article CAS Google Scholar
  9. Bean, B. P. & Friel, D. D. Ion Channels 2, 169–203 (1990).
    Article CAS Google Scholar
  10. Bleehen, T. & Keele, C. A. Pain 3, 367–377 (1977).
    Article CAS Google Scholar
  11. Valera, S. et al. Nature 371, 516–518 (1994).
    Article ADS CAS Google Scholar
  12. Brake, A. J., Wagenbach, M. J. & Julius, D. Nature 371, 519–522 (1994).
    Article ADS CAS Google Scholar
  13. Akopian, A. & Wood, J. N. J. biol. Chem. (in the press).
  14. Huang, M. & Chalfie, M. Nature 367, 467–470 (1994).
    Article ADS CAS Google Scholar
  15. Krieg, P. A. & Melton, D. A. Nucleic Acids Res. 12, 7057–7070 (1984).
    Article CAS Google Scholar
  16. Ho, B. T., Huo, Y. Y., Lu, J. G., Newman, R. A. & Levin, V. A. Anticancer Drugs 2, 91–94 (1992).
    Article Google Scholar
  17. Bouvier, M. N., Evans, M. L. & Benham, C. D. Eur. J. Neurosci. 3, 285–291 (1991).
    Article Google Scholar
  18. Robertson, S. J., Rowan, E. R. & Kennedy, C. Br. J. Pharmac. (in the press).
  19. Lawson, S. N. in Sensory Neurons (ed. Scott, S. A.) 27–60 (Oxford Univ. Press, 1992).
    Google Scholar
  20. Canessa, C. M. et al. Nature 367, 463–467 (1994).
    Article ADS CAS Google Scholar
  21. Hoong, K. & Driscoll, M. Nature 367, 470–474 (1994).
    Article ADS Google Scholar
  22. Colquhoun, D. Lectures in Biostatistics (Oxford Univ. Press, 1971).
    MATH Google Scholar
  23. Andrews, P. W. et al. Lab. Invest. 50, 147–162 (1984).
    CAS PubMed Google Scholar
  24. Wood, J. N. et al. Proc. R. Soc. B 241, 187–194 (1990).
    Article ADS CAS Google Scholar
  25. Ninkina, N. N., Stevens, G. E., Wood, J. N. & Richardson, W. D. Nucleic Acids Res. 21, 3175–3182 (1993).
    Article CAS Google Scholar
  26. LeBeau, M.-C., Alvarez-Bolando, G., Braissant, O., Wahli, W. & Catsicas, S. Nucleic Acids Res. 19, 1337 (1991).
    Article CAS Google Scholar
  27. Escurat, M., Djabali, K., Gumpel, M., Gros, F. & Portier, M.-M. J. Neurosci. 10, 764–784 (1990).
    Article CAS Google Scholar
  28. Wood, J. N., Winter, J., James, I., Rang, H. P. & Bevan, S. J. Neurosci. 8, 3208–3220 (1988).
    Article CAS Google Scholar
  29. Schaeren-Wimers, N. & Gerfin-Moser, A. Histochemistry 100, 431–440 (1993).
    Article Google Scholar

Download references

Author information

Authors and Affiliations

  1. Departments of Anatomy and Developmental Biology, University College, London, WC1E 6BT, UK
    Chih-Cheng Chen, Armen N. Akopian, Geoffrey Burnstock & John N. Wood
  2. Department of Pharmacology, University College, London, WC1E 6BT, UK
    Lucia Sivilottit & David Colquhoun

Authors

  1. Chih-Cheng Chen
    You can also search for this author inPubMed Google Scholar
  2. Armen N. Akopian
    You can also search for this author inPubMed Google Scholar
  3. Lucia Sivilottit
    You can also search for this author inPubMed Google Scholar
  4. David Colquhoun
    You can also search for this author inPubMed Google Scholar
  5. Geoffrey Burnstock
    You can also search for this author inPubMed Google Scholar
  6. John N. Wood
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Chen, CC., Akopian, A., Sivilottit, L. et al. A P2X purinoceptor expressed by a subset of sensory neurons.Nature 377, 428–431 (1995). https://doi.org/10.1038/377428a0

Download citation

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.