Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese (original) (raw)

References

  1. Nicholls, D. G. & Locke, R. M. Thermogenic mechanisms in brown fat. Physiol. Rev. 64, 1–64 (1984).
    Article CAS Google Scholar
  2. Foster, D. O. & Frydman, M. L. Tissue distribution of cold-induced thermogenesis in conscious warm- or cold-acclimated rats reevaluated from changes in tissue blood flow: the dominant role of brown adipose tissue in the replacement of shivering by nonshivering thermogenesis. Can. J. Physiol. Pharmacol. 57, 257–270 (1979).
    Article CAS Google Scholar
  3. Rothwell, N. J. & Stock, M. J. A role for brown adipose tissue in diet-induced thermogenesis. Nature 281, 31–35 (1979).
    Article ADS CAS Google Scholar
  4. Champigny, O. et al. B3-Adrenergic receptor stimualtion restores message and expression of brown-fat mitochondrial uncoupling protein in adult dogs. Proc. Natl Acad. Sci. USA 88, 10774–10777 (1991).
    Article ADS CAS Google Scholar
  5. Himms-Hagen, J. et al. Effect of CL-316,243, a thermogenic B3-agonist, on energy balance and brown and white adipose tissues in rat. Am. J. Physiol. 266, R1371–R1382 (1994).
    CAS PubMed Google Scholar
  6. Kozak, L. P., Kozak, U. C. & Clarke, G. T. Abnormal brown and white fat development in transgenic mice overexpressing glycerol 3-phosphate dehydrogenase. Genes Dev. 5, 2256–2264 (1991).
    Article CAS Google Scholar
  7. Lowell, B. B. et al. Development of obesity in transgenic mice after genetic ablation of brown adipose tissue. Nature 366, 740–742 (1993).
    Article ADS CAS Google Scholar
  8. Kopecky, J., Clarke, G., Enerback, S., Spiegelman, B. & Kozak, L. P. Expression of the mitochondrial uncoupling protein gene from the aP2 promoter prevents genetic obesity. J. Clin. Invest. 96, 2914–2923 (1995).
    Article CAS Google Scholar
  9. Cummings, D. E. et al. Genetically lean mice result from targeted disruption of protein kinase A. Nature 382, 622–626 (1996).
    Article ADS CAS Google Scholar
  10. Thomas, K. R. & Capecchi, M. R. Site-directed mutagenesis by gene targeting in mouse embryoderived stem cells. Cell 51, 503–512 (1987).
    Article CAS Google Scholar
  11. Gossler, A., Doestchman, T. C., Korn, R., Serfling, E. & Kemler, R. Transgenesis by means of blastocystderived embryonic stem cell lines. Proc. Natl Acad. Sci. USA 83, 9065–9069 (1986).
    Article ADS CAS Google Scholar
  12. Kozak, L. P., Britton, J. H., Kozak, U. C. & Wells, J. M. The mitochondrial uncoupling protein gene. Correlation of exon structure to transmembrane domains. J. Biol. Chem. 263, 12274–12277 (1988).
    CAS PubMed Google Scholar
  13. Cannon, B. & Vogel, G. The mitochondrial ATPase of brown adipose tissue. Purification and comparison with mitochondria ATPase from beef heart. FEBS Lett. 76, 284–289 (1977).
    Article CAS Google Scholar
  14. Susulic, V. S. et al. Targeted disruption of the β3-adrenergic receptor gene. J. Biol. Chem 270, 29483–29492 (1995).
    Article CAS Google Scholar
  15. Holloway, B. R. et al. ICI D7114 a novel selective β-adrenoceptor agonist selectively stimulates brown fat and increases whole body oxygen consumption. Br. J. Pharmacol. 104, 97–104 (1991).
    Article CAS Google Scholar
  16. Hamann, A., Flier, J. S. & Lowell, B. B. Decreased brown fat markedly enhances susceptibility to dietinduced obesity, diabetes and hyperlipidemia . Endocrinology 137, 21–29 (1996).
    Article CAS Google Scholar
  17. Billington, C. J., Briggs, J. E., Grace, M. & Levine, A. S. Effects of intracerebroventricular injection of neuropeptide Y on energy metabolism. Am. J. Physiol. 266, R1765–R1770 (1991).
    Google Scholar
  18. Koza, R. A. et al. Sequence and tissue-dependent RNA expression of mouse FAD-linked glycerol-3-phosphate dehydrogenase. Arch. Biochem. Biophys. 336, 97–104 (1996).
    Article CAS Google Scholar
  19. Melnyk, A., Harper, M.-E. & Himms-Hagen, J. Raising at thermoneutrality prevents obesity and hyperphagia in brown adipose tissue-ablated transgenic mice. Am. J. Physiol. 272, R1088–R1093 (1997).
    CAS PubMed Google Scholar
  20. Leibel, R. L., Rosenbaum, M. & Hirsch, J. Changes in energy expenditure resulting from altered body weight. N. Engl. J. Med. 332, 621–628 (1995).
    Article CAS Google Scholar
  21. Lean, M. E. J. & James, W. P. T. in Brown Adipose Tissue (eds Trayhurn, P. & Nicholls, D. G.) 339–365 (Arnold, London, 1986).
    Google Scholar
  22. Soriano, P., Montgomery, C., Geske, R. & Bradley, A. Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell 64, 693– 702 (1991).
    Article CAS Google Scholar
  23. Chomczynski, P. & Sacchi, N. Single-step method of RNA isolation by acid guanidium thiocyanatephenol-chloroform extraction. Analyt. Biochem. 162, 156–159 (1987).
    Article CAS Google Scholar
  24. Derman, E. et al. Transcriptional control in the production of liver-specific mRNAs. Cell 23, 731–739 (1981).
    Article CAS Google Scholar
  25. Feinberg, A. P. & Vogelstein, B. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analyt. Biochem. 132, 6–13 (1983).
    Article CAS Google Scholar
  26. Zeviani, M. et al. Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase. Gene 65, 1–11 (1988).
    Article CAS Google Scholar
  27. Bernlohr, D. A., Angus, C. W., Lane, M. D., Bolanowski, M. A. & Kelly, T. J. J. Expression of specific mRNAs during adipose differentiation: identification of an mRNA encoding a homologue of myelin P2 protein. Proc. Natl Acad. Sci. USA 81, 5468–5472 (1984).
    Article ADS CAS Google Scholar
  28. Kozak, L. P. & Birkenmeier, E. H. Mouse _sn_-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence. Proc. Natl Sci. USA 80, 3020–3024 (1983).
    Article ADS CAS Google Scholar
  29. Kyte, J. & Doolittle, R. F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105–132 (1982).
    Article CAS Google Scholar
  30. Ma, S. W. & Foster, D. O. Starvation-induced changes in metabolic rate, blood flow, and regional energy expenditure in rats. Can. J. Physiol. Pharmacol. 64, 1252–1258 (1986).
    Article CAS Google Scholar
  31. Thomas, S. A. & Palmiter, R. D. Thermoregulatory and metabolic phenotypes of mice lacking noradrenaline and adrenaline Nature 387, 94–97 (1997).
    Article ADS CAS Google Scholar

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