Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes (original) (raw)

References

  1. Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811 (1998).
    Article CAS PubMed Google Scholar
  2. Montgomery, M.K., Xu, S. & Fire, A. RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans. Proc. Natl Acad. Sci. USA 95, 15502 –15507 (1998).
    Article CAS PubMed PubMed Central Google Scholar
  3. Timmons, L. & Fire, A. Specific interference by ingested dsRNA . Nature 395, 854 (1998).
  4. Montgomery, M.K. & Fire, A. Double-stranded RNA as a mediator in sequence-specific genetic silencing and co-suppression. Trends Genet. 14, 255–258 (1998).
    Article CAS PubMed Google Scholar
  5. Tabara, H., Grishok, A. & Mello, C.C. RNAi in _C. elegans_—soaking in the genome sequence. Science 282, 430– 431 (1998).
    Article CAS PubMed Google Scholar
  6. Jones, D., Russnak, R.H., Kay, R.J. & Candido, E.P.M. Structure, expression and evolution of a heat-shock gene locus in C. elegans that is flanked by repetitive elements. J. Biol. Chem. 261 , 12006–12015 (1986).
    CAS PubMed Google Scholar
  7. Stringham, E.G., Dixon, D.K., Jones, D. & Candido, E.P.M. Temporal and spatial expression patterns of the small heat shock (hsp-16) genes in transgenic Caenorhabditis elegans. Mol. Biol. Cell 3, 221–233 (1992).
    Article CAS PubMed PubMed Central Google Scholar
  8. Fire, A., Harrison, S.W. & Dixon, D. A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans. Gene 93 , 189–198 (1990).
    Article CAS PubMed Google Scholar
  9. Lu, X. & Horvitz, H.R. lin-35 and lin-53, two genes that antagonize a C. elegans Ras pathway, encode proteins similar to Rb and its binding protein RbAp48. Cell 95, 981–991 (1998).
    Article CAS PubMed Google Scholar
  10. Zhang Y., LeRoy, G., Seelig, H.P., Lane, W.S. & Reinberg, D. The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities. Cell 95, 279–289 (1998).
    Article CAS PubMed Google Scholar
  11. Ryazanov, A.G. et al. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc. Natl Acad. Sci. USA 94, 4884–4889 ( 1997).
    Article CAS PubMed PubMed Central Google Scholar
  12. Maduro, M. & Pilgrim, D. Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system. Genetics 141, 977–988 ( 1995).
    CAS PubMed PubMed Central Google Scholar
  13. Ren, X.-C, Kim, S., Fox, E., Hedgecock, E. & Wadsworth, W.G. Role of netrin UNC-6 in patterning the longitudinal nerves of Caenorhabditis elegans. J. Neurobiol. 39, 107–118 (1999).
    Article CAS PubMed Google Scholar
  14. Mitani, S., Du, H., Hall, D., Driscoll, M. & Chalfie, M. Combinatorial control of touch receptor neuron expression in Caenorhabditis elegans. Development 119, 773–783 (1993).
    CAS PubMed Google Scholar
  15. Park, E.-C & Horvitz, H.R. Mutations with dominant effects on the behavior and morphology of the nematode C. elegans. Genetics 113, 821–852 ( 1986).
    CAS PubMed PubMed Central Google Scholar
  16. Dibb, N.J., Maruyama, I.N., Krause, M. & Karn, J. Sequence analysis of the complete Caenorhabditis elegans myosin heavy chain gene family. J. Mol. Biol. 205, 603– 613 (1989).
    Article CAS PubMed Google Scholar
  17. Kennerdell, J.R. & Carthew, R.W. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 95, 1017– 1026 (1998).
    Article CAS PubMed Google Scholar
  18. Ngo, H., Tschudi, C., Gull, K. & Ullu, E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc. Natl Acad. Sci. USA 95, 14687–14692 (1998).
    Article CAS PubMed PubMed Central Google Scholar
  19. Voinnet, O., Vain, P., Angell, V. & Baulcombe, D.C. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell 95, 177–187 (1998).
    Article CAS PubMed Google Scholar
  20. Sánchez Alvarado, A. & Newmark, P.A. Double-stranded RNA specifically disrupts gene expression during planarian regeneration. Proc. Natl Acad. Sci. USA 96, 5049– 5054 (1999).
    Article PubMed PubMed Central Google Scholar
  21. Waterhouse, P.M., Graham, M.W. & Wang, M.B. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc. Natl. Acad. Sci. USA 95, 13959– 13964 (1998).
    Article CAS PubMed PubMed Central Google Scholar
  22. Brenner, S. The genetics of Caenorhabditis elegans. Genetics 77, 71–94 (1974).
    CAS PubMed PubMed Central Google Scholar
  23. Mello, C.C. & Fire, A. DNA transformation. in Methods in Cell Biology. Caenorhabditis elegans: Modern Biological Analysis of an Organism (eds Epstein, H.F. & Shakes, D.C. ) 451–482 (Academic Press, San Diego, 1995).
  24. Kramer, J.M., French, R.P., Park, E.-C & Johnson, J.J. The Caenorhabditis elegans rol-6 gene, which interacts with the sqt-1 collagen gene to determine organismal morphology, encodes a collagen. Mol. Cell. Biol. 10, 2081–2089 (1990).
    Article CAS PubMed PubMed Central Google Scholar
  25. Tabara, H. et al. The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123– 132 (1999).
    Article CAS PubMed Google Scholar

Download references