Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients (original) (raw)

Cell Research volume 22, pages 248–258 (2012)Cite this article

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Abstract

Gastric cancer is the fourth most common cancer worldwide, with a high rate of death and low 5-year survival rate. To date, there is a lack of efficient therapeutic protocols for gastric cancer. Recent studies suggest that cancer stem cells (CSCs) are responsible for tumor initiation, invasion, metastasis, and resistance to anticancer therapies. Thus, therapies that target gastric CSCs are attractive. However, CSCs in human gastric adenocarcinoma (GAC) have not been described. Here, we identify CSCs in tumor tissues and peripheral blood from GAC patients. CSCs of human GAC (GCSCs) that are isolated from tumor tissues and peripheral blood of patients carried CD44 and CD54 surface markers, generated tumors that highly resemble the original human tumors when injected into immunodeficient mice, differentiated into gastric epithelial cells in vitro, and self-renewed in vivo and in vitro. Our findings suggest that effective therapeutic protocols must target GCSCs. The capture of GCSCs from the circulation of GAC patients also shows great potential for identification of a critical cell population potentially responsible for tumor metastasis, and provides an effective protocol for early diagnosis and longitudinal monitoring of gastric cancer.

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Acknowledgements

The relevant institutional Ethics Committees approved this study. We thank the participants and their families for their kind cooperation, generosity, and patience. This work was supported by the project of National Basic Research Program of China (2007CB947802, 2009CB941200).

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Author notes

  1. Tie Chen and Kun Yang: These two authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China
    Tie Chen, Jianhua Yu, Wentong Meng, Fang Liu, Fan Zeng, Hong Xu & Xianming Mo
  2. Department of Gastrointestinal Surgery, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China
    Kun Yang, Jie Liu, Bing Dai, Xinzu Chen, Fang Wang & Jiankun Hu
  3. Oncology, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China
    Dandan Yuan & Feng Bi

Authors

  1. Tie Chen
  2. Kun Yang
  3. Jianhua Yu
  4. Wentong Meng
  5. Dandan Yuan
  6. Feng Bi
  7. Fang Liu
  8. Jie Liu
  9. Bing Dai
  10. Xinzu Chen
  11. Fang Wang
  12. Fan Zeng
  13. Hong Xu
  14. Jiankun Hu
  15. Xianming Mo

Corresponding authors

Correspondence toJiankun Hu or Xianming Mo.

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( Supplementary information is linked to the online version of the paper on the Cell Research website.)

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Chen, T., Yang, K., Yu, J. et al. Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients.Cell Res 22, 248–258 (2012). https://doi.org/10.1038/cr.2011.109

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