A B-cell epigenetic signature defines three biologic subgroups of chronic lymphocytic leukemia with clinical impact (original) (raw)

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Acknowledgements

We are grateful to S Guijarro, S Martín, C Capdevila, M Sánchez, L Plà, A Heydorn and G Riesen for excellent technical assistance, and to N Villahoz and C Muro for excellent work in the coordination of the Spanish CLL Genome Consortium. We are indebted to the Hospital Clínic de Barcelona–IDIBAPS Biobank-Tumor Bank and Hematopathology Collection for the sample procurement as well as to the technical and medical staff of all the laboratories involved in the study. We are also very grateful to the patients with CLL who have participated in this study. This work was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) through the Instituto de Salud Carlos III (ISCIII) and the Red Temática de Investigación del Cáncer (RTICC) of the ISCIII (RD12/0036/0036 to EC, RD12/0036/0023 to AL-G, RD12/0036/0004 to DC and RD12/0036/0067 to CL-O) and project SAF2009-08663 (JIM-S), the UK Medical Research Council (AM, SJ and MJSD) as well as the European Union’s Seventh Framework Programme through the Blueprint Consortium (grant agreement 282510 to EC and RS). CL-O is a researcher of the Botín Foundation and EC an ICREA-Academia researcher. JIM-S is supported by a Ramon y Cajal contract of the MINECO, ACQ by the Portuguese Fundação para a Ciência e a Tecnologia, MK by the Agència de Gestió d’Ajuts Universitaris i de Recerca (Generalitat de Catalunya) and JR by a Junior Excellence Research Group of the Jackstädt foundation.

Author Contributions

ACQ, MK, AN, JR, AKB, JK, CR, NR and ML-G performed experiments. ACQ, NV, AM-T, G Clot, G Castellano, SB, IS, XSP, RS, CR, AL-G and JIM-S analyzed and interpreted data. EMM-P, MP and MA performed sample preparation and quality control. NV, AM-T, SJ, AM, DC, MA, MR, JD, EG, MG-D and MJSD reviewed the pathologic and clinical data and confirmed diagnosis. CL-O, EC and JIM-S designed the study. ACQ, NV, EC, AL-G and JIM-S wrote the manuscript. All authors read and approved the final version of the manuscript.

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Author notes

1. A C Queirós and N Villamor: These authors contributed equally to this study as first authors.
2. A López-Guillermo and J I Martín-Subero: These authors contributed equally to this study as senior authors.

Authors and Affiliations

1. Departamento de Anatomía Patológica, Unidad de Hematopatología, Servicio de Anatomía Patológica, Hospital Clínic, Farmacología y Microbiología, Universitat de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
   A C Queirós, N Villamor, G Clot, M Kulis, A Navarro, C Royo, N Russiñol, G Castellano, M Pinyol, S Bea, I Salaverria, M López-Guerra, D Colomer, M Aymerich, M Rozman, E Campo & J I Martín-Subero
2. Servicio de Hematología, Hospital Clínic de Barcelona, IDIBAPS, Barcelona, Spain
   A Martinez-Trillos, J Delgado, E Giné & A López-Guillermo
3. Institute of Human Genetics, Christian-Albrechts-University of Kiel, University Hospital Schleswig-Holstein, Campus Kiel, Germany
   E M M Penas, J Richter, A K Bergmann, J Kolarova & R Siebert
4. MRC Toxicology Unit Leicester, Leicester, UK
   S Jayne & A Majid
5. Servicio de Hematología, Hospital Universitario, Centro de Investigación del Cáncer-IBMCC (USAL-CSIC), Universidad de Salamanca, Salamanca, Spain,
   M González-Díaz
6. Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Spain
   X S Puente & C López-Otín
7. Ernest and Helen Scott Haematological Research Institute, Leicester University, Leicester, UK
   M J S Dyer
8. Josep Carreras Leukemia Research Institute, Barcelona, Spain
   C Rozman

Authors

1. A C Queirós
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2. N Villamor
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3. G Clot
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4. A Martinez-Trillos
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5. M Kulis
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6. A Navarro
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7. E M M Penas
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8. S Jayne
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9. A Majid
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10. J Richter
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11. A K Bergmann
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12. J Kolarova
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13. C Royo
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14. N Russiñol
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15. G Castellano
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16. M Pinyol
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17. S Bea
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18. I Salaverria
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19. M López-Guerra
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20. D Colomer
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21. M Aymerich
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22. M Rozman
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23. J Delgado
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24. E Giné
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25. M González-Díaz
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26. X S Puente
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27. R Siebert
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28. M J S Dyer
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29. C López-Otín
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30. C Rozman
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31. E Campo
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32. A López-Guillermo
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33. J I Martín-Subero
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Corresponding authors

Correspondence toA López-Guillermo or J I Martín-Subero.

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The authors declare no conflict of interest.

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Queirós, A., Villamor, N., Clot, G. et al. A B-cell epigenetic signature defines three biologic subgroups of chronic lymphocytic leukemia with clinical impact.Leukemia 29, 598–605 (2015). https://doi.org/10.1038/leu.2014.252

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• Received: 14 January 2014
• Revised: 01 August 2014
• Accepted: 11 August 2014
• Published: 25 August 2014
• Issue Date: March 2015
• DOI: https://doi.org/10.1038/leu.2014.252