Functional profiling of the Saccharomyces cerevisiae genome (original) (raw)
References
- Wach, A., Brachat, A. & Phillippsen, P. Guidelines for EUROFAN B0 Program: ORF deletants, plasmid tools, basic functional analyses. EUROFAN [online] 〈http://mips.gsf.de/proj/eurofan/eurofan_1/b0/home_requisites/guideline/sixpack.html〉 (1996).
- Winzeler, E. A. et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285, 901–906 (1999)
Article CAS Google Scholar - Ross-Macdonald, P. et al. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 402, 413–418 (1999)
Article ADS CAS Google Scholar - Piano, F., Schetterdagger, A. J., Mangone, M., Stein, L. & Kemphues, K. J. RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Curr. Biol. 10, 1619–1622 (2000)
Article CAS Google Scholar - Fraser, A. G. et al. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330 (2000)
Article ADS CAS Google Scholar - Liu, L. X. et al. High-throughput isolation of Caenorhabditis elegans deletion mutants. Genome Res. 9, 859–867 (1999)
Article CAS Google Scholar - Zambrowicz, B. P. et al. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature 392, 608–611 (1998)
Article ADS CAS Google Scholar - Hamer, L. et al. Gene discovery and gene function assignment in filamentous fungi. Proc. Natl Acad. Sci. USA 98, 5110–5115 (2001)
Article ADS CAS Google Scholar - Wach, A., Brachat, A., Pohlmann, R. & Philippsen, P. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793–1808 (1994)
Article CAS Google Scholar - Mewes, H. W. et al. MIPS: a database for genomes and protein sequences. Nucleic Acids Res. 28, 37–40 (2000)
Article ADS CAS Google Scholar - Jones, E. W. & Fink, G. R. in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression (eds Strathern, J. N., Jones, E. W. & Broach, J. R.) 181–300 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1982)
Google Scholar - Posas, F. et al. Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 “two-component” osmosensor. Cell 86, 865–875 (1996)
Article CAS Google Scholar - Yale, J. & Bohnert, H. J. Transcript expression in Saccharomyces cerevisiae at high salinity. J. Biol. Chem. 276, 15996–16007 (2001)
Article CAS Google Scholar - Poon, P. P. et al. Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein. Proc. Natl Acad. Sci. USA 93, 10074–10077 (1996)
Article ADS CAS Google Scholar - Causton, H. C. et al. Remodeling of yeast genome expression in response to environmental changes. Mol. Biol. Cell 12, 323–337 (2001)
Article CAS Google Scholar - Roth, F. P., Hughes, J. D., Estep, P. W. & Church, G. M. Finding DNA regulatory motifs within unaligned noncoding sequences clustered by whole-genome mRNA quantitation. Nature Biotechnol. 16, 939–945 (1998)
Article CAS Google Scholar - Li, N. & Snyder, M. A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol. Biol. Cell 12, 2147–2170 (2001)
Article Google Scholar - Benzer, S. On the topography of the genetic fine structure. Proc. Natl Acad. Sci. USA 47, 403–415 (1961)
Article ADS CAS Google Scholar - Sherman, F., Fink, G. R. & Hinks, J. B. Methods in Yeast Genetics 145–149 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1986)
Google Scholar - Guthrie, C. & Fink, G. R. (eds) Guide to Yeast Genetics and Molecular Biology 12–15 (Academic, San Diego, California, 1991)
- Hoffman, C. S. & Winston, F. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57, 267–272 (1987)
Article CAS Google Scholar - Eisen, M. B., Spellman, P. T., Brown, P. O. & Botstein, D. Cluster analysis and display of genome-wide expression patterns. Proc. Natl Acad. Sci. USA 95, 14863–14868 (1998)
Article ADS CAS Google Scholar
Acknowledgements
We thank I. Bastiaens, J. Howard Dees, R. Diaz, F. Dietrich, K. Freidel, N. Liebundguth, C. Rebischong, R. Schiavon, J. Schneider, T. Verhoeven and R. Wysoki for technical assistance. G.G. thanks C. Nislow for critical readings of the manuscript. This work was primarily supported by grants from the European Commission and the National Human Genome Research Institute (USA), the Medical Research Council of Canada, and the Swiss Office for Science.
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Authors and Affiliations
- Stanford Genome Technology Center, Stanford University, Palo Alto, California, 94304, USA
Guri Giaever, Keith Anderson, Karen Davis, Zelek Herman, Daniel F. Jaramillo & Ronald W. Davis - Department of Biochemistry, Stanford University School of Medicine, Stanford, California, 94305-5307, USA
Angela M. Chu, Anna Astromoff, Adam Deutschbauer, Hong Liang, Daniel D. Shoemaker, Elizabeth A. Winzeler, Grace Yen & Ronald W. Davis - Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, Connecticut, 06520-8103, USA
Li Ni, Rhonda Bangham, Darlene LaBonte, Hong Liao, Lucy Liu, Chuanyun Luo, Petra Ross-Macdonald, Yonghong Yang & Michael Snyder - Developmental Biology, Yale University,
Li Ni, Rhonda Bangham, Darlene LaBonte, Hong Liao, Lucy Liu, Chuanyun Luo, Petra Ross-Macdonald, Yonghong Yang & Michael Snyder - Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520-8103, USA
Mark Gerstein & Ning Lan - Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205-2185, USA
Carla Connelly, Rong Mao, Siew Loon Ooi, Sharon Sookhai-Mahadeo, Elaine Youngman, Kexin Yu & Jef D. Boeke - Genetics, Johns Hopkins University School of Medicine,
Carla Connelly, Rong Mao, Siew Loon Ooi, Sharon Sookhai-Mahadeo, Elaine Youngman, Kexin Yu & Jef D. Boeke - Department of Genetics, Washington University Medical School, St Louis, Missouri, 63110, USA
Linda Riles, Matt Curtiss, Julie Wilhelmy & Mark Johnston - Department of Biology, McGill University, Montreal, Québec, H3A 1B1, Canada
Steeve Véronneau, Marc Lussier, Patrice Menard & Howard Bussey - Rosetta Inpharmatics Inc., Washington, 98034, Kirkland, USA
Sally Dow, Christopher J. Roberts, Greg Schimmack & Teresa R. Ward - FYSA, Université catholique de Louvain, Place Croix du Sud, 2/20, 1348, Louvain-la-Neuve, Belgium
Ankuta Lucau-Danila & Francoise Foury - Université Libre de Bruxelles, Laboratoire de Physiologie Cellulaire, IBMM CP300, Gosselies, Belgium
Bruno André - Departments of Bioengineering and Chemistry, University of California, Berkeley
Adam P. Arkin & Patrick Flaherty - Physical Biosciences Division, Lawrence Berkeley National Laboratory, Howard Hughes Medical Institute, California, 94720-1770, Berkeley, USA
Adam P. Arkin & Patrick Flaherty - IRMW, Université Libre de Bruxelles, Brussels, B-1070, Belgium
Mohamed El Bakkoury & Bart Scherens - Departamento de Microbiologia y Genetica, Instituto de Microbiologia y Bioquimica, CSIC/Universidad de Salamanca, E-37007, Salamanca, Spain
Rocio Benito & Jose L. Revuelta - Department of Molecular Microbiology, Biozentrum, University of Basel, CH-4056, Basel, Switzerland
Sophie Brachat & Peter Philippsen - Department of Biology, University of Padova, I-35121, Padova, Italy
Stefano Campanaro & Giorgio Valle - EUROSCARF, Johann Wolfgang Goethe-Universität, Institute of Microbiology, Frankfurt/Main, D-60439, Germany
Karl-Dieter Entian, Svenja Hempel, Peter Kötter & Matthias Rose - Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, California, 94720-1770, USA
Patrick Flaherty - Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland, 21702, USA
David J. Garfinkel, Deanna Gotte, Brenda Shafer, Jeffrey N. Strathern & Ching-yun Wang - Institut fur Mikrobiologie, Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, D-40225, Germany
Ulrich Güldener & Johannes H. Hegemann - Institute of Biological Sciences, University of Wales, UK, Aberystwyth, Wales, SY23 3DA
Diane E. Kelly, Steven L. Kelly & David C. Lamb - Department of Biology, Concordia University, Canada, Montreal, Québec, H3G 1M8
Reginald K. Storms - Katholieke Universiteit Leuven, Laboratory of Gene Technology, Leuven, B-3001, Belgium
Marleen Voet & Guido Volckaert
Authors
- Guri Giaever
- Angela M. Chu
- Li Ni
- Carla Connelly
- Linda Riles
- Steeve Véronneau
- Sally Dow
- Ankuta Lucau-Danila
- Keith Anderson
- Bruno André
- Adam P. Arkin
- Anna Astromoff
- Mohamed El Bakkoury
- Rhonda Bangham
- Rocio Benito
- Sophie Brachat
- Stefano Campanaro
- Matt Curtiss
- Karen Davis
- Adam Deutschbauer
- Karl-Dieter Entian
- Patrick Flaherty
- Francoise Foury
- David J. Garfinkel
- Mark Gerstein
- Deanna Gotte
- Ulrich Güldener
- Johannes H. Hegemann
- Svenja Hempel
- Zelek Herman
- Daniel F. Jaramillo
- Diane E. Kelly
- Steven L. Kelly
- Peter Kötter
- Darlene LaBonte
- David C. Lamb
- Ning Lan
- Hong Liang
- Hong Liao
- Lucy Liu
- Chuanyun Luo
- Marc Lussier
- Rong Mao
- Patrice Menard
- Siew Loon Ooi
- Jose L. Revuelta
- Christopher J. Roberts
- Matthias Rose
- Petra Ross-Macdonald
- Bart Scherens
- Greg Schimmack
- Brenda Shafer
- Daniel D. Shoemaker
- Sharon Sookhai-Mahadeo
- Reginald K. Storms
- Jeffrey N. Strathern
- Giorgio Valle
- Marleen Voet
- Guido Volckaert
- Ching-yun Wang
- Teresa R. Ward
- Julie Wilhelmy
- Elizabeth A. Winzeler
- Yonghong Yang
- Grace Yen
- Elaine Youngman
- Kexin Yu
- Howard Bussey
- Jef D. Boeke
- Michael Snyder
- Peter Philippsen
- Ronald W. Davis
- Mark Johnston
Corresponding author
Correspondence toRonald W. Davis.
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The authors declare that they have no competing financial interests.
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Giaever, G., Chu, A., Ni, L. et al. Functional profiling of the Saccharomyces cerevisiae genome.Nature 418, 387–391 (2002). https://doi.org/10.1038/nature00935
- Received: 19 March 2002
- Accepted: 19 June 2002
- Issue Date: 25 July 2002
- DOI: https://doi.org/10.1038/nature00935