The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments (original) (raw)

References

  1. Sung, P., Trujillo, K. M. & Van Komen, S. Recombination factors of Saccharomyces cerevisiae. Mutat. Res. 451, 257–275 (2000)
    Article CAS Google Scholar
  2. Aguilera, A. & Klein, H. L. Genetic control of intrachromosomal recombination in Saccharomyces cerevisiae. I. Isolation and genetic characterization of hyper-recombination mutations. Genetics 119, 779–790 (1988)
    CAS PubMed PubMed Central Google Scholar
  3. Aboussekhra, A. et al. RADH, a gene of Saccharomyces cerevisiae encoding a putative DNA helicase involved in DNA repair. Characteristics of radH mutants and sequence of the gene. Nucleic Acids Res. 17, 7211–7219 (1989)
    Article CAS Google Scholar
  4. Kaytor, M. D., Nguyen, M. & Livingston, D. M. The complexity of the interaction between RAD52 and SRS2. Genetics 140, 1441–1442 (1995)
    CAS PubMed PubMed Central Google Scholar
  5. Milne, G. T., Ho, T. & Weaver, D. T. Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51. Genetics 139, 1189–1199 (1995)
    CAS PubMed PubMed Central Google Scholar
  6. Schild, D. Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or mating-type heterozygosity. Genetics 140, 115–127 (1995)
    CAS PubMed PubMed Central Google Scholar
  7. Chanet, R., Heude, M., Adjiri, A., Maloisel, L. & Fabre, F. Semidominant mutations in the yeast Rad51 protein and their relationships with the Srs2 helicase. Mol. Cell. Biol. 16, 4782–4789 (1996)
    Article CAS Google Scholar
  8. Fabre, F., Chan, A., Heyer, W. D. & Gangloff, S. Alternate pathways involving Sgs1/Top3, Mus81/Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication. Proc. Natl Acad. Sci. USA 99, 1687–1692 (2002)
    Article Google Scholar
  9. Klein, H. L. Mutations in recombinational repair and in checkpoint control genes suppress the lethal combination of srs2Δ with other DNA repair genes in Saccharomyces cerevisiae. Genetics 157, 557–565 (2001)
    CAS PubMed PubMed Central Google Scholar
  10. Singleton, M. R. & Wigley, D. B. Modularity and specialization in superfamily 1 and 2 helicases. J. Bacteriol. 184, 1819–1826 (2002)
    Article CAS Google Scholar
  11. Rong, L. & Klein, H. L. Purification and characterization of the Srs2 DNA helicase of the yeast Saccharomyces cerevisiae. J. Biol. Chem. 268, 1252–1259 (1993)
    CAS PubMed Google Scholar
  12. Petukhova, G., Stratton, S. & Sung, P. Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature 393, 91–94 (1998)
    Article ADS CAS Google Scholar
  13. Krejci, L. et al. DNA helicase Srs2 disrupts formation of the Rad51 presynaptic filament. Nature 423, 305–309 (2003)
    Article ADS CAS Google Scholar
  14. Aboussekhra, A., Chanet, R., Adjiri, A. & Fabre, F. Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins. Mol. Cell. Biol. 12, 3224–3234 (1992)
    Article CAS Google Scholar
  15. Lovett, S. T. & Mortimer, R. K. Characterization of null mutants of the RAD55 gene of Saccharomyces cerevisiae: effects of temperature, osmotic strength and mating type. Genetics 116, 547–553 (1987)
    CAS PubMed PubMed Central Google Scholar
  16. Vaze, M. et al. Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase. Mol. Cell 10, 373 (2002)
    Article CAS Google Scholar
  17. Palladino, F. & Klein, H. L. Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutants. Genetics 132, 23–37 (1992)
    CAS PubMed PubMed Central Google Scholar
  18. Heude, M., Chanet, R. & Fabre, F. Regulation of the Saccharomyces cerevisiae Srs2 helicase during the mitotic cell cycle, meiosis and after irradiation. Mol. Gen. Genet. 248, 59–68 (1995)
    Article CAS Google Scholar
  19. Van Komen, S., Petukhova, G., Sigurdsson, S., Stratton, S. & Sung, P. Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54. Mol. Cell 6, 563–572 (2000)
    Article CAS Google Scholar
  20. Gangloff, S., Soustelle, C. & Fabre, F. Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicases. Nature Genet. 25, 192–194 (2000)
    Article CAS Google Scholar
  21. van Brabant, A. J., Stan, R. & Ellis, N. A. DNA helicases, genomic instability, and human genetic disease. Annu. Rev. Genomics Hum. Genet. 1, 409–459 (2000)
    Article CAS Google Scholar
  22. Saintigny, Y., Makienko, K., Swanson, C., Emond, M. J. & Monnat, R. J. Jr Homologous recombination resolution defect in Werner syndrome. Mol. Cell. Biol. 22, 6971–6978 (2002)
    Article CAS Google Scholar
  23. Iggo, R. D. & Lane, D. P. Nuclear protein p68 is an RNA-dependent ATPase. EMBO J. 8, 1827–1831 (1989)
    Article CAS Google Scholar
  24. Beloin, C. et al. Contribution of DNA conformation and topology in right-handed DNA wrapping by the Bacillus subtilis LrpC protein. J. Biol. Chem. 278, 5333–5342 (2003)
    Article CAS Google Scholar

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