Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans (original) (raw)

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Acknowledgements

We thank M. Srayko, C. Cowan, L. Pelletier and W. B. Wood for critical reading of the manuscript, and M. Volkmer and S. Schloissnig for their help on the completion of the online Phenobank database. This work was supported in part by the German government through a DLR grant. A.C. was supported by the MRC, UK.

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Author notes

1. L. B. Koski
   Present address: Centre Robert Cedergren, Centre de Recherche en Bioinformatique et en Sciences Génomiques de l'Université de Montréal, 2900 Edouard-Montpetit, Montreal, Quebec, H3T 1J4, Canada
2. P. Marschall
   Present address: Max Planck Institute of Infection Biology, Schumannstrasse 21/22, 10117, Berlin, Germany
3. B. Neumann
   Present address: European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117, Heidelberg, Germany
4. A.-M. Alleaume
   Present address: Institut Cochin, Département de Biologie Cellulaire, 22 rue Méchain, 75014, Paris, France
5. J. Artelt
   Present address: Institut für Klinische Genetik, Medizinische Fakultät Carl Gustav Carus, TU Dresden, Fetscherstrasse 74, 01307, Dresden, Germany
6. P. Bettencourt
   Present address: Instituto Gulbenkian de Ciência, Rua da Quinta Grande n 6, 2780-901, Oeiras, Portugal
7. E. Cassin
   Present address: Cavaleri Ottolenghi Scientific Institut (COSI) of Neurobiology, Azienda Ospedale San Luigi Gonzaga, Regione Gonzole 10-1004, Orbassano, Italy
8. B. Nitzsche
   Present address: , Mühlstrasse 36, 04317, Leipzig, Germany
9. R. Heinkel
   Present address: , Brombeerweg 2, 76275, Ettlingen, Germany
10. M. Röder
    Present address: Ingeniweb, 2 cours du 14 Juillet, 78300, Poissy, France
11. J. Finell
    Present address: Rätiälänkatu 20 A 11, 20810, Turku, Finland
12. M. Jones
    Present address: Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
13. K. Oegema
    Present address: University of California, San Diego, CMM-East 3080, 9500 Gilman Drive, La Jolla, California, 92093, USA
14. P. Gönczy
    Present address: Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Lausanne, Switzerland
15. A. Coulson
    Present address: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

Authors and Affiliations

1. Cenix BioScience GmbH, Tatzberg 47-51, D-01307, Dresden, Germany
   B. Sönnichsen, L. B. Koski, A. Walsh, P. Marschall, B. Neumann, M. Brehm, A.-M. Alleaume, J. Artelt, P. Bettencourt, M. Hewitson, C. Holz, M. Khan, S. Lazik, C. Martin, B. Nitzsche, M. Winzi, R. Heinkel, M. Röder, J. Finell, H. Häntsch & C. J. Echeverri
2. Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG), Pfotenhauerstrasse 108, D-01307, Dresden, Germany
   E. Cassin, M. Ruer, J. Stamford, K. Oegema, P. Gönczy & A. A. Hyman
3. Genome Sciences Centre, British Columbia Cancer Research Centre, 570 West 7th Avenue, Vancouver, British Columbia, V5Z 4E6, Canada
   S. J. M. Jones
4. The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK
   M. Jones & A. Coulson
5. Department of Biology, Centre for Comparative Functional Genomics, New York University, 1009 Silver Centre, 100 Washington Square East, New York, New York, 10003, USA
   F. Piano & K. C. Gunsalus

Authors

1. B. Sönnichsen
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2. L. B. Koski
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3. A. Walsh
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4. P. Marschall
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5. B. Neumann
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6. M. Brehm
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7. A.-M. Alleaume
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8. J. Artelt
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9. P. Bettencourt
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10. E. Cassin
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11. M. Hewitson
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12. C. Holz
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13. M. Khan
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14. S. Lazik
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15. C. Martin
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16. B. Nitzsche
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17. M. Ruer
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18. J. Stamford
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19. M. Winzi
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20. R. Heinkel
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21. M. Röder
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22. J. Finell
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23. H. Häntsch
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24. S. J. M. Jones
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25. M. Jones
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26. F. Piano
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27. K. C. Gunsalus
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28. K. Oegema
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29. P. Gönczy
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30. A. Coulson
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31. A. A. Hyman
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32. C. J. Echeverri
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Corresponding author

Correspondence toB. Sönnichsen.

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The authors declare that they have no competing financial interests.

Supplementary information

Supplementary Table 1

Phenotypic and functional classification of all genes with early embryonic (DIC) phenotypes. (XLS 337 kb)

Supplementary Table 2

Comparison between RNAi and conventional genetics. (XLS 26 kb)

Supplementary Table 3

Comparison of screening coverage and detection accuracy for early embryonic phenotypes in the present study versus Zipperlen et al. and Piano et al. (XLS 25 kb)

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Sönnichsen, B., Koski, L., Walsh, A. et al. Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.Nature 434, 462–469 (2005). https://doi.org/10.1038/nature03353

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• Received: 27 August 2004
• Accepted: 10 January 2005
• Issue Date: 24 March 2005
• DOI: https://doi.org/10.1038/nature03353