A novel class of small RNAs bind to MILI protein in mouse testes (original) (raw)

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Acknowledgements

We thank J. Brennecke, M. Landthaler, Y. Pei, K. Fejes Toth and A. Sewer for discussion and help with the manuscript. We also thank L. Mitchell and K. Hwang for expert assistance in germ-cell purification, M. Poy and M. Stoffel for providing mouse tissues, and D. Weir and R. Choksi for assistance with sequencing. This work was supported by a FRAXA Research Foundation postdoctoral fellowship to A.A., an NIH grant to P.M., NIH grants to T.T., and an SNF grant to M.Z. Author Contributions A.A., S.P. and M.L.-Q. prepared the mouse, and P.L. and N.I. the human, testes small RNA libraries. A.A. recognized the presence of piRNAs, performed the MILI IPs, prepared the MILI-interacting small RNA library, and performed, together with N.I., the northern blotting analysis. S.K.-M. and T.N. produced, characterized and purified the MILI antibody. T.T. developed the concept of cloning from Ago/Piwi IPs. P.M. isolated germline cells. M.C., J.J.R. and J.J. performed the large-scale sequencing. The bioinformatic analyses of piRNAs were designed and carried out by D.G. and M.Z. with input from A.A. and T.T. The database of RNAs with known function was compiled by M.Z., R.S., P.L. and S.P., the software used for small RNA annotation was developed by M.Z., R.S. and C.S., and the manuscript was written by A.A., M.Z. and T.T.

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Author notes

1. Alexei Aravin
   Present address: Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York, 11724, USA
2. Sébastien Pfeffer
   Present address: CNRS-UPR 2357, IBMP, 12 rue du Général Zimmer, 67084, Strasbourg Cedex, France
3. Alexei Aravin and Dimos Gaidatzis: *These authors contributed equally to this work

Authors and Affiliations

1. Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, 1230 York Avenue, Box 186, New York, 10021, USA
   Alexei Aravin, Sébastien Pfeffer, Mariana Lagos-Quintana, Pablo Landgraf, Nicola Iovino & Thomas Tuschl
2. Biozentrum, Universität Basel, Basel, Klingelbergstr 50-70, CH-4056, Switzerland
   Dimos Gaidatzis & Mihaela Zavolan
3. Population Council, The Rockefeller University, New York, 1230 York Avenue, New York, 10021, USA
   Patricia Morris
4. J. Craig Venter Institute, Functional Genomics, Rockville, 9704 Medical Center Drive, Maryland, 20850, USA
   Michael J. Brownstein
5. Department of Pathology, Medical School, Graduate School of Frontier Biosciences, Osaka University, Osaka, Yamada-oka 2-2 Suita, 565-0871, Japan
   Satomi Kuramochi-Miyagawa & Toru Nakano
6. Columbia Genome Center, Russ Berrie Pavilion, New York, 1150 St. Nicholas Avenue, New York, 10032, USA
   Minchen Chien, James J. Russo & Jingyue Ju
7. Department of Chemical Engineering, Columbia University, New York, 500 West 120 Street, New York, 10027, USA
   Jingyue Ju
8. Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, New York, 10021, USA
   Robert Sheridan & Chris Sander
9. Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York, 11724, USA
   Sébastien Pfeffer
10. CNRS-UPR 2357, IBMP, 12 rue du Général Zimmer, 67084, Strasbourg Cedex, France
    Alexei Aravin

Authors

1. Alexei Aravin
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2. Dimos Gaidatzis
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3. Sébastien Pfeffer
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4. Mariana Lagos-Quintana
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5. Pablo Landgraf
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6. Nicola Iovino
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7. Patricia Morris
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8. Michael J. Brownstein
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9. Satomi Kuramochi-Miyagawa
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10. Toru Nakano
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11. Minchen Chien
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12. James J. Russo
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13. Jingyue Ju
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14. Robert Sheridan
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15. Chris Sander
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16. Mihaela Zavolan
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17. Thomas Tuschl
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Corresponding authors

Correspondence toMihaela Zavolan or Thomas Tuschl.

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Competing interests

Sequences of the piRNAs determined in this paper are given in Supplementary Tables 4 and 9. Reprints and permissions information is available at npg.nature.com/reprintsandpermissions. The authors declare no competing financial interests.

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Aravin, A., Gaidatzis, D., Pfeffer, S. et al. A novel class of small RNAs bind to MILI protein in mouse testes.Nature 442, 203–207 (2006). https://doi.org/10.1038/nature04916

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• Received: 18 February 2006
• Accepted: 17 May 2006
• Published: 04 June 2006
• Issue Date: 13 July 2006
• DOI: https://doi.org/10.1038/nature04916