Phylogenomics reveals deep molluscan relationships (original) (raw)

Accession codes

Data deposits

Capillary sequence data are available from the NCBI EST database (http://www.ncbi.nlm.nih.gov/projects/dbEST) under accession numbers JG454968.1–JG456874.1 and 454 sequence data are available from the NCBI SRA database (http://www.ncbi.nlm.nih.gov/sra) accession number SRA030407.1. Matrices and trees from this study are available from TreeBASE (http://www.treebase.org) accession number S11762.

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Acknowledgements

We thank W. Jones and K. T. Fielman for help with cDNA library preparation, R. M. Jennings, N. Mikkelsen, and the crews of the RV Håkon Mosby, RV Hans Brattstrom and RV Laurence M. Gould for assistance collecting aplacophorans, and J. C. Havird, P. J. Krug, S. C. Kempf, D. R. Lindberg, M. V. Matz, L. R. Page and T. H. Struck for discussions. D. Speiser kindly shared the photo of Argopecten. F. W. Goetz, A. Gracey and M. L. Blaxter kindly provided sequence quality data for Dreissena rostriformis, Mytilus californianus and Lumbricus rubellus, respectively. We thank A. Di Cosmo, P. Burbach, V. Rehder, W. Wright and R. Gillette for providing samples of Octopus, Loligo, Helisoma, Dolabrifera and Pleurobranchaea as well as sharing some sequencing cost for these species. We also thank D. Young and the Alabama Supercomputer Authority for access to computational resources. The genomes of Capitella teleta, Helobdella robusta, Lottia gigantea and Nematostella vectensis were produced by the US Department of Energy Joint Genome Institute in collaboration with the user community. This work was supported by National Science Foundation (NSF) grants (0744649 and 0821622) to K.M.H., National Institute of Health (NIH) grants (1RO1NS06076, 1R01GM097502, R21 RR025699, R21DA030118) and the McKnight Brain Research Foundation to L.L.M., the Deep Metazoan Phylogeny (DMP) program of the German Science Foundation (Li 998/9-1) to B.L., and The University of Bergen (Norway) free researcher initiated project grant to C.T. (project no. 226270). This work represents contributions 82 and 4 to the Auburn University (AU) Marine Biology Program and Molette Biology Laboratory for Environmental and Climate Change Studies, respectively.

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Authors and Affiliations

  1. Department of Biological Sciences, Auburn University, 101 Rouse Life Sciences, Auburn, 36849, Alabama, USA
    Kevin M. Kocot, Johanna T. Cannon, Scott R. Santos & Kenneth M. Halanych
  2. Department of Biology and Centre for Geobiology, University of Bergen, P.O. Box 7800, NO-5020 Bergen, Norway
    Christiane Todt & Christoffer Schander
  3. The Whitney Laboratory for Marine Bioscience, University of Florida, 9505 Ocean Shore Blvd., St. Augustine, 32080, Florida, USA
    Mathew R. Citarella, Andrea B. Kohn & Leonid L. Moroz
  4. Institute of Zoology, Johannes Gutenberg University, Müllerweg 6, D-55099 Mainz, Germany
    Achim Meyer & Bernhard Lieb
  5. Department of Neuroscience, University of Florida, Gainesville, 32611, Florida, USA
    Leonid L. Moroz

Authors

  1. Kevin M. Kocot
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  2. Johanna T. Cannon
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  3. Christiane Todt
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  4. Mathew R. Citarella
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  5. Andrea B. Kohn
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  6. Achim Meyer
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  7. Scott R. Santos
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  8. Christoffer Schander
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  9. Leonid L. Moroz
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  10. Bernhard Lieb
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  11. Kenneth M. Halanych
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Contributions

K.M.H., C.T., B.L., C.S. and K.M.K. conceived and designed this study. K.M.H., L.L.M., B.L. and C.T. supervised cDNA preparation and sequencing. L.L.M., A.B.K., K.M.K., J.T.C. and A.M. prepared and sequenced cDNA. K.M.K., J.T.C., S.R.S. and M.R.C. developed the bioinformatics pipeline. K.M.K. performed phylogenetic and ancestral state reconstruction analyses. K.M.K. and J.T.C. prepared the figures. C.S., C.T. and K.M.K. modified the morphological character matrix. A.B.K., K.M.K. and A.M. submitted sequences to GenBank. All authors contributed in preparing the Letter.

Corresponding authors

Correspondence toKevin M. Kocot or Kenneth M. Halanych.

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The authors declare no competing financial interests.

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Kocot, K., Cannon, J., Todt, C. et al. Phylogenomics reveals deep molluscan relationships.Nature 477, 452–456 (2011). https://doi.org/10.1038/nature10382

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