Phosphorylation of NLRC4 is critical for inflammasome activation (original) (raw)

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Acknowledgements

We thank P. Broz, A. Paler Martinez, M. Roose-Girma, X. Rairdan, C. Kung, V. Asghari and S. Mukund for technical support, and K. O’Rourke for editorial assistance.

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Author notes

  1. Yan Qu and Shahram Misaghi: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Physiological Chemistry, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA,
    Yan Qu, Kim Newton, Nobuhiko Kayagaki & Vishva M. Dixit
  2. Department of Early Stage Cell Culture, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA,
    Shahram Misaghi & Salina Louie
  3. Department of Protein Chemistry, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA,
    Anita Izrael-Tomasevic & David Arnott
  4. Department of Immunology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA,
    Laurie L. Gilmour & James E. Cupp
  5. Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium,
    Mohamed Lamkanfi
  6. Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium,
    Mohamed Lamkanfi
  7. Department of Bioinformatics and Computational BiologyGenentech Inc., 1 DNA Way, South San Francisco, 94080, California, USA
    Jinfeng Liu
  8. Department of Pathology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA,
    László Kömüves
  9. Department of Microbiology and Immunology, Stanford School of Medicine, 94305, California, USA
    Denise Monack

Authors

  1. Yan Qu
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  2. Shahram Misaghi
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  3. Anita Izrael-Tomasevic
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  4. Kim Newton
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  5. Laurie L. Gilmour
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  6. Mohamed Lamkanfi
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  7. Salina Louie
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  8. Nobuhiko Kayagaki
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  9. Jinfeng Liu
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  10. László Kömüves
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  11. James E. Cupp
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  12. David Arnott
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  13. Denise Monack
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  14. Vishva M. Dixit
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Contributions

Y.Q., S.M., A.I.-T., D.A., L.L.G., J.E.C., L.K. and S.L. designed and conducted experiments; K.N. generated the Nlrc4 F/F mice; J.L. performed bioinformatics analyses; M.L., N.K. and D.M. discussed the study; Y.Q., K.N. and V.M.D. wrote the manuscript.

Corresponding author

Correspondence toVishva M. Dixit.

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Competing interests

Y.Q., S.M., A.I.-T., K.N., L.L.G., J.E.C., S.L., N.K., J.L., L.K., D.A. and V.M.D. are employees of Genentech, Inc.

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Qu, Y., Misaghi, S., Izrael-Tomasevic, A. et al. Phosphorylation of NLRC4 is critical for inflammasome activation.Nature 490, 539–542 (2012). https://doi.org/10.1038/nature11429

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