Human embryonic stem cells reveal recurrent genomic instability at 20q11.21 (original) (raw)
- Brief Communication
- Published: 23 November 2008
- Maxime Feyeux1,
- Cécile Bas2,
- Olivier Féraud3,
- Annelise Bennaceur-Griscelli3,
- Gerard Tachdjian2,
- Marc Peschanski1 &
- …
- Anselme L Perrier1
Nature Biotechnology volume 26, pages 1364–1366 (2008)Cite this article
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Abstract
By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5–4.6 Mb at 20q11.21, encompassing ∼23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture.
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Figure 1: Genomic instability at 20q11.21 in SA01.
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Acknowledgements
This study has been supported in part by additional grants from Medicen Paris Region (IngeCELL), EC (FP6, STEM-HD) and ANR (HESCREEN). The authors thank K. Sermon (AZ-VUB, Brussels) for kindly providing VUB cell lines.
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Authors and Affiliations
- INSERM/UEVE UMR-861, I-STEM, AFM, Institute for Stem cell Therapy and Exploration of Monogenic diseases, 5 rue Henri Desbruères, Evry, 91030, cedex, France
Nathalie Lefort, Maxime Feyeux, Marc Peschanski & Anselme L Perrier - Service de biologie et génétique de la reproduction, INSERM U782, Hôpital Antoine Béclère, Université Paris 11, Clamart, France
Cécile Bas & Gerard Tachdjian - INSERM U602/Université Paris-Sud 11, Hôpital Paul Brousse, Villejuif, France
Olivier Féraud & Annelise Bennaceur-Griscelli
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- Nathalie Lefort
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Contributions
N.L., M.P. and A.L.P. designed the study. N.L. carried out and analyzed CGH and SNP experiments. C.B. carried out the FISH experiments. N.L. and M.F. did all hES cell culture, except for that of H1 cells, which were cultured by O.F. The FISH experiments were designed and analyzed by G.T., A.B.-G. and C.B. A.B.-G. and G.T. edited the paper. N.L., M.P. and A.L.P. contributed to writing the paper.
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Correspondence toAnselme L Perrier.
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Lefort, N., Feyeux, M., Bas, C. et al. Human embryonic stem cells reveal recurrent genomic instability at 20q11.21.Nat Biotechnol 26, 1364–1366 (2008). https://doi.org/10.1038/nbt.1509
- Received: 20 June 2008
- Accepted: 30 October 2008
- Published: 23 November 2008
- Issue Date: December 2008
- DOI: https://doi.org/10.1038/nbt.1509