Targeted gene disruption in somatic zebrafish cells using engineered TALENs (original) (raw)

To the Editor:

Miller et al.1 recently described a transcription activator–like effector nuclease (TALEN) architecture for efficient genome editing in cultured human cells. We sought to determine whether the same framework could be used to efficiently disrupt endogenous genes in somatic cells of zebrafish and how the efficiency of TALENs compares with that obtained using engineered zinc-finger nucleases (ZFNs).

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Acknowledgements

We thank D. Voytas and A. Bogdanove for helpful discussions in the early stages of this project and J. Foley, M. Maeder, S. Thibodeau-Beganny, F. Zhang, M. Christian and D. Voytas for help with characterizing the _hey2_- and _gria3a_-targeted ZFN pairs. This work was supported by US National Institutes of Health (NIH) R01 GM088040 (J.K.J. & R.T.P.), NIH Director's Pioneer Award DP1 OD006862 (J.K.J.), NIH T32 CA009216 (J.D.S.), NIH K01 AG031300 (J.-R.J.Y.), the Jim and Ann Orr Massachusetts General Hospital (MGH) Research Scholar award (J.K.J.), and the Charles and Ann Sanders MGH Research Scholar award (R.T.P.).

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Author notes

  1. Jeffry D Sander and Lindsay Cade: These authors contributed equally to this work.

Authors and Affiliations

  1. Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Jeffry D Sander, Cyd Khayter & J Keith Joung
  2. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Jeffry D Sander & J Keith Joung
  3. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Lindsay Cade, Randall T Peterson & Jing-Ruey J Yeh
  4. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Lindsay Cade, Randall T Peterson & Jing-Ruey J Yeh
  5. Department of Genetics, Development and Cell Biology,
    Deepak Reyon
  6. Interdepartmental Graduate Program in Bioinformatics & Computational Biology, Iowa State University, Ames, Iowa, USA
    Deepak Reyon
  7. Broad Institute, Cambridge, Massachusetts, USA
    Randall T Peterson

Authors

  1. Jeffry D Sander
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  2. Lindsay Cade
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  3. Cyd Khayter
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  4. Deepak Reyon
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  5. Randall T Peterson
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  6. J Keith Joung
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  7. Jing-Ruey J Yeh
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Corresponding authors

Correspondence toJ Keith Joung or Jing-Ruey J Yeh.

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The authors declare no competing financial interests.

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Sander, J., Cade, L., Khayter, C. et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs.Nat Biotechnol 29, 697–698 (2011). https://doi.org/10.1038/nbt.1934

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