Epigenetic regulation of gene structure and function with a cell-permeable Cre recombinase (original) (raw)

References

  1. Nagy, A. Cre recombinase: the universal reagent for genome tailoring. Genetics 26, 99–109 (2000).
    CAS Google Scholar
  2. Sauer, B. Inducible gene targeting in mice using the Cre/lox system. Methods 14, 381–392 (1998).
    Article CAS Google Scholar
  3. Gu, H., Marth, J.D., Orban, P.C., Mossmann, H. & Rajewsky, K. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265, 103–106 (1994).
    Article CAS Google Scholar
  4. Betz, U.A., Vosshenrich, C.A., Rajewsky, K. & Muller, W. Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination. Curr. Biol. 6, 1307–1316 (1996).
    Article CAS Google Scholar
  5. Kuhn, R., Schwenk, F., Aguet, M. & Rajewsky, K. Inducible gene targeting in mice. Science 269, 1427–1429 (1995).
    Article CAS Google Scholar
  6. Zou, Y.R., Muller, W., Gu, H. & Rajewsky, K. Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies. Curr. Biol. 4, 1099–1103 (1994).
    Article CAS Google Scholar
  7. Sauer, B. & Henderson, N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 2, 441–449 (1990).
    CAS PubMed Google Scholar
  8. Smith, A.J. et al. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nat. Genet. 9, 376–385 (1995).
    Article CAS Google Scholar
  9. Van Deursen, J., Fornerod, M., Van Rees, B. & Grosveld, G. Cre-mediated site-specific translocation between nonhomologous mouse chromosomes. Proc. Natl. Acad. Sci. USA 92, 7376–7380 (1995).
    Article CAS Google Scholar
  10. Justice, M.J., Zheng, B., Woychik, R.P. & Bradley, A. Using targeted large deletions and high-efficiency _N_-ethyl-_N_-nitrosourea mutagenesis for functional analyses of the mammalian genome. Methods 13, 423–436 (1997).
    Article CAS Google Scholar
  11. Zheng, B. et al. Engineering a mouse balancer chromosome. Nat. Genet. 22, 375–378 (1999).
    Article CAS Google Scholar
  12. Su, H., Wang, X. & Bradley, A. Nested chromosomal deletions induced with retroviral vectors in mice. Nat. Genet. 24, 92–95 (2000).
    Article CAS Google Scholar
  13. Lakso, M. et al. Targeted oncogene activation by site-specific recombination in transgenic mice. Proc. Natl. Acad. Sci. USA 89, 6232–6236 (1992).
    Article CAS Google Scholar
  14. Fiering, S., Kim, C.G., Epner, E.M. & Groudine, M. An “in–out” strategy using gene targeting and FLP recombinase for the functional dissection of complex DNA regulatory elements: analysis of the beta-globin locus control region. Proc. Natl. Acad. Sci. USA 90, 8469–8473 (1993).
    Article CAS Google Scholar
  15. Russ, A.P. et al. Identification of genes induced by factor deprivation in hematopoietic cells undergoing apoptosis using gene-trap mutagenesis and site-specific recombination. Proc. Natl. Acad. Sci. USA 93, 15279–15284 (1996).
    Article CAS Google Scholar
  16. Lin, Y.Z., Yao, S.Y., Veach, R.A., Torgerson, T.R. & Hawiger, J. Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence. J. Biol. Chem. 270, 14255–14258 (1995).
    Article CAS Google Scholar
  17. Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat. Genet. 21, 70–71 (1999).
    Article CAS Google Scholar
  18. Anton, M. & Graham, F.L. Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression. J. Virol. 69, 4600–4606 (1995).
    CAS PubMed PubMed Central Google Scholar
  19. Kanegae, Y. et al. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 23, 3816–3821 (1995).
    Article CAS Google Scholar
  20. Rohlmann, A., Gotthardt, M., Willnow, T.E., Hammer, R.E. & Herz, J. Sustained somatic gene inactivation by viral transfer of Cre recombinase. Nat. Biotechnol. 14, 1562–1565 (1996).
    Article CAS Google Scholar
  21. Feil, R. et al. Ligand-activated site-specific recombination in mice. Proc. Natl. Acad. Sci. USA 93, 10887–10890 (1996).
    Article CAS Google Scholar
  22. Kellendonk, C. et al. Regulation of Cre recombinase activity by the synthetic steroid RU 486. Nucleic Acids Res. 24, 1404–1411 (1996).
    Article CAS Google Scholar
  23. Schwarze, S.R., Ho, A., Vocero-Akbani, A. & Dowdy, S.F. In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 285, 1569–1572 (1999).
    Article CAS Google Scholar
  24. Yan Liu, X. et al. Peptide-directed suppression of a pro-inflammatory cytokine response. J. Biol. Chem. 275, 16774–16778 (2000).
    Article Google Scholar
  25. Perez, F. et al. Antennapedia homeobox as a signal for the cellular internalization and nuclear addressing of a small exogenous peptide. J. Cell. Sci. 102, 717–722 (1992).
    CAS PubMed Google Scholar
  26. Hawiger, J. Noninvasive intracellular delivery of functional peptides and proteins. Curr. Opin. Chem. Biol. 3, 89–94 (1999).
    Article CAS Google Scholar
  27. Schwartz, J.J. & Zhang, S. Peptide-mediated cellular delivery. Curr. Opin. Mol. Ther. 2, 162–167. (2000).
    CAS PubMed Google Scholar
  28. Schwarze, S.R., Hruska, K.A. & Dowdy, S.F. Protein transduction: unrestricted delivery into all cells? Trends Cell Biol. 10, 290–295 (2000).
    Article CAS Google Scholar
  29. Rojas, M., Donahue, J.P., Tan, Z. & Lin, Y.Z. Genetic engineering of proteins with cell membrane permeability. Nat. Biotechnol. 16, 370–375 (1998).
    Article CAS Google Scholar
  30. Kolb, A.F. & Siddell, S.G. Genomic targeting with an MBP–Cre fusion protein. Gene 183, 53–60 (1996).
    Article CAS Google Scholar
  31. Morgenstern, J.P. & Land, H. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 18, 3587–3596 (1990).
    Article CAS Google Scholar
  32. Pear, W.S., Nolan, G.P., Scott, M.L. & Baltimore, D. Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA 90, 8392–8396 (1993).
    Article CAS Google Scholar
  33. Beddinton, R.S. & Lawson, K.A. In Postimplantation mammalian embryos. (eds Copp, A.J. & Cockroft, D.L.) 267–292 (IRL Press, New York, NY; 1990).
    Google Scholar

Download references