Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase (original) (raw)

Nature Biotechnology volume 22, pages 321–325 (2004)Cite this article

Abstract

Small interfering RNAs (siRNA) are potent reagents for directed post-transcriptional gene silencing1 and a major new genetic tool for investigating mammalian cells. When synthetic siRNAs are used for gene silencing, the costs can be substantial because of variations in siRNA efficacies. An alternative to chemically synthesized siRNAs are siRNAs produced by bacteriophage T7 RNA polymerase. We found that siRNAs synthesized from the T7 RNA polymerase system can trigger a potent induction of interferon α and β in a variety of cell lines. Surprisingly, we also found very potent induction of interferon α and β by short single-stranded RNAs (ssRNAs) transcribed with T3, T7 and Sp6 RNA polymerases. Analyses of the potential mediators of this response revealed that the initiating 5′ triphosphate is required for interferon induction. We describe here an improved method for T7 siRNA synthesis that alleviates the interferon response while maintaining full efficacy of the siRNAs.

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Acknowledgements

This work was supported by a grant from the Arnold and Mabel Beckman Foundation and the National Institutes of Health (AI29329, AI42552 and HL074704 to J.J.R.). D. Kim is a Beckman Fellow.

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Authors and Affiliations

  1. Division of Molecular Biology, City of Hope and Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, 91010-3011, California, USA
    Dong-Ho Kim, Michael Longo, Young Han & John J Rossi
  2. Division of Virology, City of Hope and Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, 91010-3011, California, USA
    Patric Lundberg & Edouard Cantin
  3. Graduate School of Biological Sciences, City of Hope and Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, 91010-3011, California, USA
    John J Rossi

Authors

  1. Dong-Ho Kim
  2. Michael Longo
  3. Young Han
  4. Patric Lundberg
  5. Edouard Cantin
  6. John J Rossi

Corresponding author

Correspondence toJohn J Rossi.

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The authors declare no competing financial interests.

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Kim, DH., Longo, M., Han, Y. et al. Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase.Nat Biotechnol 22, 321–325 (2004). https://doi.org/10.1038/nbt940

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