Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb (original) (raw)

Nature Cell Biology volume 11, pages 197–203 (2009)Cite this article

Abstract

The dominant effect of transcription factors in imparting expanded potency is best exemplified by the reprogramming of fibroblasts to pluripotent cells using retrovirus-mediated transduction of defined transcription factors. In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to convert fibroblasts to induced pluripotent stem (iPS) cells that have many characteristics of embryonic stem (ES) cells. Here we show that the orphan nuclear receptor Esrrb functions in conjunction with Oct4 and Sox2 to mediate reprogramming of mouse embryonic fibroblasts (MEFs) to iPS cells. Esrrb-reprogrammed cells share similar expression and epigenetic signatures as ES cells. These cells are also pluripotent and can differentiate in vitro and in vivo into the three major embryonic cell lineages. Furthermore, these cells contribute to mouse chimaeras and are germline transmissible. In ES cells, Esrrb targets many genes involved in self-renewal and pluripotency. This suggests that Esrrb may mediate reprogramming through the upregulation of ES-cell-specific genes. Our findings also indicate that it is possible to reprogram MEFs without exogenous Klf transcription factors and link a nuclear receptor to somatic cell reprogramming.

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Acknowledgements

We are grateful to the Biomedical Research Council (BMRC), Agency for Science, Technology and Research (A*STAR) and Singapore Stem Cell Consortium for funding. J.J is supported by the Singapore Millennium Foundation graduate scholarship and the NUS graduate scholarship. Y.L. and J.N. are supported by the A*STAR graduate scholarship. J.H and Y.C are supported by the NUS graduate school scholarship. We are grateful to Kuee-Theng Kuay for technical support. We thank Andrew Hutchins, Tara Huber and Edwin Cheung for critical comments on the manuscript.

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Author notes

  1. Bo Feng and Jianming Jiang: These authors contributed equally to this work.

Authors and Affiliations

  1. Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, #02-01, Genome Building, Singapore, 138672
    Bo Feng, Jianming Jiang, Jia-Hui Ng, Jian-Chien Dominic Heng, Yun-Shen Chan, Lai-Ping Yaw, Weiwei Zhang, Yuin-Han Loh & Huck-Hui Ng
  2. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543
    Jianming Jiang, Jian-Chien Dominic Heng, Yun-Shen Chan, Weiwei Zhang, Yuin-Han Loh & Huck-Hui Ng
  3. Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, #02-01, Genome Building, Singapore, 138672
    Petra Kraus, Jianyong Han, Bing Lim & Thomas Lufkin
  4. Computational and Mathematical Biology, 60 Biopolis Street, #02-01, Genome Building, Genome Institute of Singapore, Singapore, 138672
    Vinsensius B. Vega
  5. Cancer Biology & Pharmacology, Genome Institute of Singapore, 60 Biopolis Street, #02-01, Genome Building, Singapore, 138672
    Valere Cacheux-Rataboul
  6. Harvard Institutes of Medicine, Harvard Medical School, Boston, 02115, MA, USA
    Bing Lim

Authors

  1. Bo Feng
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  2. Jianming Jiang
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  3. Petra Kraus
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  4. Jia-Hui Ng
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  5. Jian-Chien Dominic Heng
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  6. Yun-Shen Chan
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  7. Lai-Ping Yaw
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  8. Weiwei Zhang
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  9. Yuin-Han Loh
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  10. Jianyong Han
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  11. Vinsensius B. Vega
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  12. Valere Cacheux-Rataboul
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  13. Bing Lim
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  14. Thomas Lufkin
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  15. Huck-Hui Ng
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Correspondence toHuck-Hui Ng.

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Feng, B., Jiang, J., Kraus, P. et al. Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb.Nat Cell Biol 11, 197–203 (2009). https://doi.org/10.1038/ncb1827

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