Somatic RHOA mutation in angioimmunoblastic T cell lymphoma (original) (raw)

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Acknowledgements

We thank Y. Okoshi, N. Obara, Y. Yokoyama, H. Nishikii, N. Kurita and M. Seki for contributing to sample collection and banking. We also thank Y. Sakashita and T. Takahashi for technical assistance, and S. Narumiya and I. Kitabayashi for efficient discussion. This work was supported by Grants-in-Aid for Scientific Research (KAKENHI) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (22134006 to S.O.; 22130002, 24390241, 25112703 and 25670444 to S.C.; 25461407 to M.S.-Y.) and was supported by the Sagawa Foundation for Promotion of Cancer Research, the Naito Foundation, the Mochida Memorial Foundation for Medical and Pharmaceutical Research (M.S.-Y.).

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Author notes

1. Mamiko Sakata-Yanagimoto, Terukazu Enami and Kenichi Yoshida: These authors contributed equally to this work.
2. Seishi Ogawa and Shigeru Chiba: These authors jointly directed this work.

Authors and Affiliations

1. Department of Hematology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan
   Mamiko Sakata-Yanagimoto, Terukazu Enami, Yasuyuki Miyake, Hideharu Muto, Yuhei Kamada, Rie Nakamoto-Matsubara, Nguyen Bich Tran, Kazumi Suzukawa, Yuichi Hasegawa & Shigeru Chiba
2. Cancer Genomics Project, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
   Kenichi Yoshida, Aiko Sato-Otsubo, Yusuke Okuno, Yusuke Sato, Masashi Sanada & Seishi Ogawa
3. Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
   Kenichi Yoshida, Aiko Sato-Otsubo, Yusuke Sato, Masashi Sanada & Seishi Ogawa
4. Laboratory of DNA Information Analysis, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
   Yuichi Shiraishi, Kenichi Chiba & Satoru Miyano
5. Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japan
   Ryohei Ishii & Osamu Nureki
6. Division of Pathology, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan
   Naoko Tsuyama & Kengo Takeuchi
7. Pathology Project for Molecular Targets, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan
   Seiji Sakata & Kengo Takeuchi
8. Department of Hematology, Toranomon Hospital, Tokyo, Japan
   Koji Izutsu
9. Okinaka Memorial Institute for Medical Research, Tokyo, Japan
   Koji Izutsu
10. Department of Pathology, Toranomon Hospital, Tokyo, Japan
    Yasunori Ohta
11. Department of Dermatology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan
    Junichi Furuta
12. Department of Hematology, Tsuchiura Kyodo General Hospital, Tsuchiura, Japan
    Seiichi Shimizu
13. Department of Hematology, Mito Medical Center, National Hospital Organization, Mito, Japan
    Takuya Komeno
14. Department of Hematology, Tsukuba Memorial Hospital, Tsukuba, Japan
    Yuji Sato
15. Department of Hematology, JA Toride Medical Center, Toride, Japan
    Takayoshi Ito
16. Department of Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan
    Masayuki Noguchi
17. Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan
    Emiko Noguchi
18. Laboratory of Sequence Analysis, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Hiroko Tanaka & Satoru Miyano
19. Department of Clinical Laboratory, University of Tsukuba Hospital, Tsukuba, Japan
    Kazumi Suzukawa & Toru Nanmoku
20. Department of Pathology, Tokai University School of Medicine, Isehara, Japan
    Naoya Nakamura
21. Life Science Center, Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Japan
    Shigeru Chiba

Authors

1. Mamiko Sakata-Yanagimoto
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2. Terukazu Enami
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3. Kenichi Yoshida
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4. Yuichi Shiraishi
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5. Ryohei Ishii
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6. Yasuyuki Miyake
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7. Hideharu Muto
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8. Naoko Tsuyama
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9. Aiko Sato-Otsubo
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10. Yusuke Okuno
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11. Seiji Sakata
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12. Yuhei Kamada
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13. Rie Nakamoto-Matsubara
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14. Nguyen Bich Tran
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15. Koji Izutsu
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16. Yusuke Sato
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17. Yasunori Ohta
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18. Junichi Furuta
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19. Seiichi Shimizu
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20. Takuya Komeno
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21. Yuji Sato
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22. Takayoshi Ito
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23. Masayuki Noguchi
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24. Emiko Noguchi
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25. Masashi Sanada
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26. Kenichi Chiba
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27. Hiroko Tanaka
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28. Kazumi Suzukawa
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29. Toru Nanmoku
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30. Yuichi Hasegawa
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31. Osamu Nureki
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32. Satoru Miyano
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33. Naoya Nakamura
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34. Kengo Takeuchi
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35. Seishi Ogawa
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36. Shigeru Chiba
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Contributions

M.S.-Y. prepared DNA samples, sorted the tumor cells, resequenced the samples, and sorted and integrated information. T.E. analyzed the function of wild-type and mutant RHOA. K.Y. resequenced the samples and contributed to the resequencing data analyses. Y. Shiraishi, E.N., K.C., H.T. and S.M. performed bioinformatics analyses of the resequencing data. R.I. and O.N. created the model structure for mutant RHOA. Y.M., H.M., Y.K., R.N.-M., N.B.T., K.S., T.N., Y.H. and M.N. contributed to sample collection and preparation. N.T., S. Sakata, N.N. and K.T. immunostained specimens and performed pathohistological analyses. Y. Okuno and M.S. contributed to the resequencing. A.S.-O. and Yusuke Sato contributed to mRNA sequencing. K.I., Y. Ohta, J.F., S. Shimizu, T.K., Yuji Sato and T.I. collected samples. M.S.-Y., T.E., K.Y., S.O. and S.C. generated figures and tables, and wrote the manuscript. All authors participated in discussions and interpretation of the data and results.

Corresponding authors

Correspondence toSeishi Ogawa or Shigeru Chiba.

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Competing interests

The authors declare no competing financial interests.

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Sakata-Yanagimoto, M., Enami, T., Yoshida, K. et al. Somatic RHOA mutation in angioimmunoblastic T cell lymphoma.Nat Genet 46, 171–175 (2014). https://doi.org/10.1038/ng.2872

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• Received: 13 May 2013
• Accepted: 12 December 2013
• Published: 12 January 2014
• Issue Date: February 2014
• DOI: https://doi.org/10.1038/ng.2872