Genetic variation in DLG5 is associated with inflammatory bowel disease (original) (raw)

Nature Genetics volume 36, pages 476–480 (2004) Cite this article

Abstract

Crohn disease and ulcerative colitis are two subphenotypes of inflammatory bowel disease (IBD), a complex disorder resulting from gene-environment interaction. We refined our previously defined linkage region for IBD on chromosome 10q23 and used positional cloning to identify genetic variants in DLG5 associated with IBD. DLG5 encodes a scaffolding protein involved in the maintenance of epithelial integrity. We identified two distinct haplotypes with a replicable distortion in transmission (P = 0.000023 and P = 0.004 for association with IBD, P = 0.00012 and P = 0.04 for association with Crohn disease). One of the risk-associated DLG5 haplotypes is distinguished from the common haplotype by a nonsynonymous single-nucleotide polymorphism 113G→A, resulting in the amino acid substitution R30Q in the DUF622 domain of DLG5. This mutation probably impedes scaffolding of DLG5. We stratified the study sample according to the presence of risk-associated CARD15 variants to study potential gene-gene interaction. We found a significant difference in association of the 113A DLG5 variant with Crohn disease in affected individuals carrying the risk-associated CARD15 alleles versus those carrying non-risk-associated CARD15 alleles. This is suggestive of a complex pattern of gene-gene interaction between DLG5 and CARD15, reflecting the complex nature of polygenic diseases. Further functional studies will evaluate the biological significance of DLG5 variants.

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Acknowledgements

We thank all cooperating clinical centers, clinicians, families and individuals with IBD and the German Crohn's and Colitis Foundation (DCCV) for their participation; J. Papp for microsatellite genotyping; A. Andersson, B. Petersen, A. Dellsén, T. Engler, M. van Giezen, Å. Jägervall, T. Kim, N. Tepe and T. Wesse for technical assistance; M. Will and T. Lu for bioinformatics support; F. Friedrichs for assistance in statistical analysis; O. Bengtsson and K. Forsman-Semb for discussions; and M. J. Daly for the Haploview software. This study was supported by the 5th Framework Program of the European Commission and the Federal Ministry of Science and Education through the National Genome Research Network and the Competence Network “Inflammatory Bowel Disease”, and by a coordinated research group of the German Research Foundation (DFGFOR423).

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Author notes

  1. Monika Stoll
    Present address: Genetic Epidemiology of Vascular Disorders, Institute for Arteriosclerosis Research, Muenster, Germany

Authors and Affiliations

  1. First Department of Medicine, University Hospital Schleswig-Holstein, Schittenhelmstr. 12, Kiel, 24105, Germany
    Monika Stoll, Christine M Costello, W Andreas Koch, Philip Rosenstiel, Peter J P Croucher, Susanna Nikolaus, Jochen Hampe, Ulrich R Foelsch & Stefan Schreiber
  2. Experimental Medicine and Department of Molecular Sciences, AstraZeneca, R&D Molndal, Sweden
    Brit Corneliussen, Bjorn Mellgard, Stefan Pierrou & Maria Lagerstrom-Fermer
  3. Conaris Research Institute AG, Kiel, Germany
    Georg H Waetzig & Dirk Seegert
  4. Max-Planck-Institute for Informatics, Saarbrücken, Germany
    Mario Albrecht & Thomas Lengauer
  5. Institute for Clinical Molecular Biology, Christian-Albrechts-University, Kiel, Germany
    Jochen Hampe & Stefan Schreiber
  6. Guy's, King's and St. Thomas' School of Medicine, London, UK
    Christopher G Mathew

Authors

  1. Monika Stoll
  2. Brit Corneliussen
  3. Christine M Costello
  4. Georg H Waetzig
  5. Bjorn Mellgard
  6. W Andreas Koch
  7. Philip Rosenstiel
  8. Mario Albrecht
  9. Peter J P Croucher
  10. Dirk Seegert
  11. Susanna Nikolaus
  12. Jochen Hampe
  13. Thomas Lengauer
  14. Stefan Pierrou
  15. Ulrich R Foelsch
  16. Christopher G Mathew
  17. Maria Lagerstrom-Fermer
  18. Stefan Schreiber

Corresponding author

Correspondence toStefan Schreiber.

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Competing interests

B.C., B.M., S.P. and M.L.-F. work for AstraZeneca, a pharmaceutical company, and G.H.W. and D.S. work for Conaris Research Institute AG. These authors have indirect interests in the intellectual property generated here.

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Stoll, M., Corneliussen, B., Costello, C. et al. Genetic variation in DLG5 is associated with inflammatory bowel disease.Nat Genet 36, 476–480 (2004). https://doi.org/10.1038/ng1345

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