Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages (original) (raw)

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Acknowledgements

We thank our colleagues of the ImmGen Project consortium; V. Jojic, J. Ericson, S. Davis and C. Benoist for contributions; eBioscience and Affymetrix for material support of the ImmGen Project; and M. Colonna (Washington University School of Medicine) for monoclonal antibodies (including anti-Siglec-H) and other reagents. Supported by the National Institute of Allergy and Infectious Diseases of the US National Institutes of Health (R24 AI072073 to fund the ImmGen Project, spearheaded by C. Benoist), the US National Institutes of Health (R01AI049653 and R01AI061741 to G.J.R.; P50GM071558-03 and R01DK08854 to A.M.; and5T32DA007135-27 to A.R.M.) and the American Heart Association (10POST4160140 to E.L.G.).

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Authors and Affiliations

  1. Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA
    Emmanuel L Gautier, Claudia Jakubzick & Gwendalyn J Randolph
  2. The Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA
    Emmanuel L Gautier, Jennifer Miller, Melanie Greter, Claudia Jakubzick, Julie Helft, Andrew Chow, Miriam Merad & Gwendalyn J Randolph
  3. Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, Missouri, USA
    Emmanuel L Gautier, Stoyan Ivanov, Wei-Jen Chua, Ted H Hansen & Gwendalyn J Randolph
  4. Broad Institute, Cambridge, Massachusetts, USA
    Tal Shay
  5. Department of Oncological Sciences and Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    Jennifer Miller, Melanie Greter, Julie Helft, Andrew Chow & Miriam Merad
  6. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA
    Kutlu G Elpek & Shannon J Turley
  7. Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Kutlu G Elpek & Shannon J Turley
  8. Department of Pharmacology and Systems Therapeutics & Systems Biology Center New York, Mount Sinai School of Medicine, New York, New York, USA
    Simon Gordonov, Amin R Mazloom & Avi Ma'ayan
  9. Icahn Medical Institute, Mount Sinai Hospital, New York, New York, USA
    Emmanuel L Gautier, Claudia Jakubzick, Gwendalyn J Randolph, Jennifer Miller, Brian Brown & Miriam Merad
  10. Department of Pathology & Immunology, Washington University, St. Louis, Missouri, USA
    Emmanuel L Gautier & Gwendalyn J Randolph
  11. Division of Biological Sciences, University of California San Diego, La Jolla, California, USA
    Adam J Best, Jamie Knell & Ananda Goldrath
  12. Computer Science Department, Stanford University, Stanford, California, USA
    Vladimir Jojic, Daphne Koller & Taras Kreslavsky
  13. Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, Massachusetts, USA
    Nadia Cohen, Patrick Brennan & Michael Brenner
  14. Broad Institute, Cambridge, Massachusetts, USA
    Tal Shay & Aviv Regev
  15. Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts, USA
    Anne Fletcher, Kutlu Elpek, Angelique Bellemare-Pelletier, Deepali Malhotra & Shannon Turley
  16. Computer Science Department, Brown University, Providence, Rhode Island, USA
    Radu Jianu & David Laidlaw
  17. Department of Biomedical Engineering, Howard Hughes Medical Institute, Boston University, Boston, Massachusetts, USA
    Jim Collins
  18. Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Kavitha Narayan, Katelyn Sylvia & Joonsoo Kang
  19. Immune Diseases Institute, Children's Hospital, Boston, Massachusetts, USA
    Roi Gazit, Brian S Garrison & Derrick J Rossi
  20. Joslin Diabetes Center, Boston, Massachusetts, USA
    Francis Kim, Tata Nageswara Rao & Amy Wagers
  21. Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    Susan A Shinton & Richard R Hardy
  22. Department of Medicine, Boston University, Boston, Massachusetts, USA
    Paul Monach
  23. Department of Microbiology & Immunology, University of California San Francisco, San Francisco, California, USA
    Natalie A Bezman, Joseph C Sun, Charlie C Kim & Lewis L Lanier
  24. Division of Immunology, Department of Microbiology & Immunobiology, Harvard Medical School, Boston, Massachusetts, USA
    Tracy Heng, Michio Painter, Jeffrey Ericson, Scott Davis, Diane Mathis & Christophe Benoist

Authors

  1. Emmanuel L Gautier
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  2. Tal Shay
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  3. Jennifer Miller
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  4. Melanie Greter
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  5. Claudia Jakubzick
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  6. Stoyan Ivanov
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  7. Julie Helft
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  8. Andrew Chow
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  9. Kutlu G Elpek
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  10. Simon Gordonov
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  11. Amin R Mazloom
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  12. Avi Ma'ayan
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  13. Wei-Jen Chua
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  14. Ted H Hansen
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  15. Shannon J Turley
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  16. Miriam Merad
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  17. Gwendalyn J Randolph
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Consortia

the Immunological Genome Consortium

Contributions

E.L.G. purified macrophage populations, designed and did experiments, analyzed data and wrote the manuscript; G.J.R. designed and supervised experiments, analyzed data and wrote the manuscript; T.S. analyzed data and wrote the manuscript; J.M. designed analytical strategies and analyzed data; M.G., C.J., J.H., A.C. and K.G.E. purified macrophage and DC populations; S.I. did experiments; S.G., A.R.M. and A.M. analyzed data; W.-J.C. and T.H.H. provided reagents and supervised experiments; and S.J.T. and M.M. designed and supervised experiments.

Corresponding author

Correspondence toGwendalyn J Randolph.

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The authors declare no competing financial interests.

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Gautier, E., Shay, T., Miller, J. et al. Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.Nat Immunol 13, 1118–1128 (2012). https://doi.org/10.1038/ni.2419

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