A role for leukocyte-endothelial adhesion mechanisms in epilepsy (original) (raw)

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Acknowledgements

Antibody to PSGL-1 (4RA10) was kindly provided by D. Vestweber, Max Plank Institute. We wish to thank C. Laudanna for critically discussing the manuscript. This work was supported in part by grants from the Fondazione Cariverona, Ministero dell'Università e della Ricerca (MIUR)–Progetti di ricerca di interese nazionale, the US National Multiple Sclerosis Society, Fondo Incentivazione Ricerca di Base, the Italian Ministry of Health; Fondazione Italiana Sclerosi Multipla, the University of Verona ex 60% MIUR (to G.C.); the European Community Research Grants LSH-CT-2006-037315 (EPICURE, thematic priority LIFESCIHEALTH; University of Verona ex 60% MIUR (to P.F.F.); and by US National Institutes of Health grants (to E.C.B., L.X., R.P.M. and J.B.L.). L.O. was supported in part by a fellowship from Fondazione Italiana Sclerosi Multipla. The authors are grateful to S. Fiorini, I. Cwojdzinski, P. Bernardi, M. Pellitteri, S. Becchi, L. Andrello and A. Calbi for their invaluable help in the experimental procedures.

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Authors and Affiliations

1. Department of Morphological-Biomedical Sciences, Section of Anatomy, Strada le Grazie 8, Verona, 37134, Italy
   Paolo F Fabene, Graciela Navarro Mora, Flavia Merigo, Donatella Benati, Asmaa Chakir, Lara Zanetti, Federica Schio, Elena Nicolato, Francesco Osculati & Andrea Sbarbati
2. Department of Pathology, Section of General Pathology, University of Verona, Strada le Grazie 8, Verona, 37134, Italy
   Marianna Martinello, Barbara Rossi, Linda Ottoboni, Simona Bach, Stefano Angiari & Gabriela Constantin
3. Department of Medicine and Public Health, Section of Forensic Medicine, University of Insubria, Via Rossi 9, 21100, Varese, Italy
   Antonio Osculati
4. Experimental Magnetic Resonance Imaging Center, Faculty of Medicine, University of Verona, Strada le Grazie 8, Verona, 37134, Italy
   Pasquina Marzola
5. Department of Pathology, University of Carolina at Chapel Hill, 420 Brinkhous-Bullitt Building, CB 7525, NC 27599-7525 Chapel Hill, 27599-7525, North Carolina, USA
   Jonathon W Homeister
6. Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, 825 Northeast Thirteenth Street, Oklahoma City, 73104, Oklahoma, USA
   Lijun Xia & Rodger P McEver
7. Department of Pathology, Case Western Reserve University, 2103 Cornell Road, Cleveland, 44106, Ohio, USA
   John B Lowe
8. Istituto di Ricovero e Cura a Carattere Scientifico Centro Neurolesi Bonino-Pulejo, Via Provinciale Palermo, Messina, 98124, Italy
   Francesco Osculati
9. Department of Pathology, Stanford University School of Medicine, Stanford, 94305-5324, California, USA
   Eugene C Butcher
10. Palo Alto VA Health Care System, 3801 Miranda Avenue, Palo Alto, 94305, California, USA
    Eugene C Butcher

Authors

1. Paolo F Fabene
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2. Graciela Navarro Mora
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3. Marianna Martinello
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4. Barbara Rossi
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5. Flavia Merigo
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6. Linda Ottoboni
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7. Simona Bach
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8. Stefano Angiari
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9. Donatella Benati
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10. Asmaa Chakir
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11. Lara Zanetti
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12. Federica Schio
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13. Antonio Osculati
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14. Pasquina Marzola
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15. Elena Nicolato
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16. Jonathon W Homeister
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17. Lijun Xia
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18. John B Lowe
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19. Rodger P McEver
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20. Francesco Osculati
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21. Andrea Sbarbati
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22. Eugene C Butcher
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23. Gabriela Constantin
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Contributions

G.N.M., D.B., A.C., L.Z. and F.S. performed epilepsy experiments, telemetry and open field behavior. M.M., B.R., L.O., S.B. and S.A. performed intravital microscopy, in vivo staining for adhesion molecules, adhesion assays and contributed to obtaining the behavioral data. A.O. provided the human samples. F.M., A.C. and F.O. performed immunohistochemistry on human and animal samples. P.M., E.N. and A.S. provided MRI expertise. J.W.H., L.X., J.B.L. and R.P.M. provided key reagents and mice. E.C.B. contributed experimental suggestions, reagents and assistance with writing. P.F.F. and G.C. designed the study, analyzed the data and wrote the paper.

Corresponding authors

Correspondence toPaolo F Fabene or Gabriela Constantin.

Supplementary information

Supplementary Text and Figures

Supplementary Figs. 1–4, Supplementary Tables 1–3, Supplementary Methods and Supplementary Discussion (PDF 587 kb)

Supplementary Video 1

This movie shows SE-induction in WT mice after pilocarpine administration paralleled by the normal behavior of mice lacking fucosyltransferase (FucT)-VII activity (MOV 2391 kb)

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Fabene, P., Mora, G., Martinello, M. et al. A role for leukocyte-endothelial adhesion mechanisms in epilepsy.Nat Med 14, 1377–1383 (2008). https://doi.org/10.1038/nm.1878

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• Received: 25 April 2008
• Accepted: 26 September 2008
• Published: 23 November 2008
• Issue Date: December 2008
• DOI: https://doi.org/10.1038/nm.1878