PKB/Akt phosphorylates p27, impairs nuclear import of p27 and opposes p27-mediated G1 arrest (original) (raw)

• Donovan, J. & Slingerland, J. Transforming growth factor-β and breast cancer: Cell cycle arrest by transforming growth factor-β and its disruption in cancer. Breast Cancer Res. 2, 116–124 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Kretzschmar, M. Transforming growth factor-β and breast cancer: Transforming growth factor-β/SMAD signaling defects and cancer. Breast Cancer Res. 2, 107–115 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Sherr, C.J. G1 phase progression: Cycling on cue. Cell 79, 551–555 (1994).
  Article CAS PubMed Google Scholar
• Sherr, C.J. & Roberts, J.M. CDK inhibitors: Positive and negative regulators of G1-phase progression. Genes Dev. 13, 1501–1512 (1999).
  Article CAS PubMed Google Scholar
• LaBaer, J. et al. New functional activities for the p21 family of CDK inhibitors. Genes Dev. 11, 847–862 (1997).
  Article CAS PubMed Google Scholar
• Cheng, M. et al. The p21(Cip1) and p27(Kip1) CDK 'inhibitors' are essential activators of cyclin D-dependent kinases in murine fibroblasts. EMBO J. 18, 1571–1583 (1999).
  Article CAS PubMed PubMed Central Google Scholar
• Koff, A., Ohtsuki, M., Polyak, K., Roberts, J.M. & Massague, J. Negative regulation of G1 in mammalian cells: Inhibition of cyclin E-dependent kinase by TGF-β. Science 260, 536–539 (1993).
  Article CAS PubMed Google Scholar
• Polyak, K. et al. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-β and contact inhibition to cell cycle arrest. Genes Dev. 8, 9–22 (1994).
  Article CAS PubMed Google Scholar
• Slingerland, J.M. et al. A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor β-arrested epithelial cells. Mol. Cell. Biol. 14, 3683–3694 (1994).
  Article CAS PubMed PubMed Central Google Scholar
• Hannon, G.J. & Beach, D. p15INK4B is a potential effector of TGF-β-induced cell cycle arrest. Nature 371, 257–261 (1994).
  Article CAS PubMed Google Scholar
• Reynisdottir, I., Polyak, K., Iavarone, A. & Massague, J. Kip/Cip and Ink4 Cdk inhibitors cooperate to induce cell cycle arrest in response to TGF-β. Genes Dev. 9, 1831–1845 (1995).
  Article CAS PubMed Google Scholar
• Sandhu, C. et al. Transforming growth factor β stabilizes p15INK4B protein, increases p15INK4B -cdk4 complexes and inhibits cyclin D1/cdk4 association in human mammary epithelial cells. Mol. Cell Biol. 17, 2458–2467 (1997).
  Article CAS PubMed PubMed Central Google Scholar
• Florenes, V.A. et al. Interleukin-6 dependent induction of the cyclin dependent kinase inhibitor p21WAF1/C1P1 is lost during progression of human malignant melanoma. Oncogene 18, 1023–1032 (1999).
  Article CAS PubMed Google Scholar
• Bouchard, C. et al. Direct induction of cyclin D2 by Myc contributes to cell cycle progression and sequestration of p27. EMBO J. 18, 5321–5333 (1999).
  Article CAS PubMed PubMed Central Google Scholar
• Cheng, M., Sexl, V., Sherr, C.J. & Roussel, M.F. Assembly of cyclin D-dependent kinase and titration of p27Kip1 regulated by mitogen-activated protein kinase kinase (MEK1). Proc. Natl. Acad. Sci. USA 95, 1091–1096 (1998).
  Article CAS PubMed PubMed Central Google Scholar
• Perez-Roger, I., Kim, S.H., Griffiths, B., Sweing, A. & Land, H. Cyclins D1 and D2 mediate Myc-induced proliferation via sequestration of p27Kip1 and p21Cip1. EMBO J. 18, 5310–5320 (1999).
  Article CAS PubMed PubMed Central Google Scholar
• Warner, B.J., Blain, S.W., Seoane, J. & Massague, J. Myc downregulation by transforming growth factor β required for activation of the p15INK4B G1 arrest pathway. Mol. Cell Biol. 19, 5913–5922 (1999).
  Article CAS PubMed PubMed Central Google Scholar
• Vlach, J., Hennecke, S., Alevizopoulos, K., Conti, D. & Amati, B. Growth arrest by the cyclin-dependent kinase inhibitor p27Kip1 is abrogated by c-Myc. EMBO J. 15, 6595–6604 (1996).
  Article CAS PubMed PubMed Central Google Scholar
• Slingerland, J. & Pagano, M. Regulation of the cdk inhibitor p27 and its deregulation in cancer. J. Cell Physiol. 183, 10–17 (2000).
  Article CAS PubMed Google Scholar
• Singh, S.P. et al. Loss or altered subcellular localization of p27 in Barrett's associated adenocarcinoma. Cancer Res. 58, 1730–1735 (1998).
  CAS PubMed Google Scholar
• Bos, J.L. Ras oncogenes in human cancer: A review. Cancer Res. 49, 4682–4689 (1989).
  CAS PubMed Google Scholar
• Di Cristofano, A. & Pandolfi, P.P. The multiple roles of PTEN in tumor suppression. Cell 100, 387–390 (2000).
  Article CAS PubMed Google Scholar
• Diehl, J.A., Cheng, M., Roussel, M.F. & Sherr, C.J. Glycogen synthase kinase-3β regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 12, 3499–3511 (1998).
  Article CAS PubMed PubMed Central Google Scholar
• Sun, H. et al. PTEN modulates cell cycle progression and cell survival by regulating phosphatidylinositol 3,4,5,trisphosphate and Akt/protein kinase B signaling pathway. Proc. Natl. Acad. Sci. USA 96, 6169–6204 (1999).
  Google Scholar
• Medema, R.H., Kops, G.J., Bos, J.L. & Burgering, B.M. AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27kip1. Nature 404, 782–787 (2000).
  Article CAS PubMed Google Scholar
• Alessi, D.R. et al. Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J. 15, 6541–6551 (1996).
  Article CAS PubMed PubMed Central Google Scholar
• Wang, Q. et al. Protein kinase B/Akt participates in GLUT4 translocation by insulin in L6 myoblasts. Mol. Cell Biol. 19, 4008–4018 (1999).
  Article CAS PubMed PubMed Central Google Scholar
• Muslin, A.J. & Xing, H. 14-3-3 proteins: Regulation of subcellular localization by molecular interference. Cell Signal 12, 703–709 (2000).
  Article CAS PubMed Google Scholar
• Obata, T. et al. Peptide and protein library screening defines optimal substrate motifs for AKT/PKB. J. Biol Chem. 275, 36108–36115 (2000).
  Article CAS PubMed Google Scholar
• Reynisdottir, I. & Massague, J. The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2. Genes Dev. 11, 492–503 (1997).
  Article CAS PubMed Google Scholar
• Zeng, Y., Hirano, K., Hirano, M., Nishimura, J. & Kanaide, H. Minimal requirements for the nuclear localization of p27Kip1, a cyclin-dependent kinase inhibitor. Biochem. Biophys. Res. Commun. 274, 37–42 (2000).
  Article CAS PubMed Google Scholar
• Jans, D.A. & Hubner, S. Regulation of protein transport to the nucleus: Central role of phosphorylation. Physiol Rev. 76, 651–685 (1996).
  Article CAS PubMed Google Scholar
• Catzavelos, C. et al. Decreased levels of the cell-cycle inhibitor p27Kip1 protein: Prognostic implications in primary breast cancer. Nature Med. 3, 227–230 (1997).
  Article CAS PubMed Google Scholar
• Zhou, B.P. et al. Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Nature Cell Biol. 3, 245–252 (2001).
  Article CAS PubMed Google Scholar
• Rossig, L. et al. Akt-Dependent Phosphorylation of p21Cip1 regulates PCNA binding and proliferation of endothelial cells. Mol. Cell Biol. 21, 5644–5657 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Li, Y., Dowbenko, D. & Lasky, L.A. AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival. J. Biol. Chem. 277, 11352–11361 (2002).
  Article CAS PubMed Google Scholar
• Tsihlias, J., Kapusta, L. & Slingerland, J. The prognostic significance of altered cyclin-dependent kinase inhibitors in human cancer. Annu. Rev. Med. 50, 401–423 (1999).
  Article CAS PubMed Google Scholar
• Yang, H.-Y., Zhou, B.P., Hung, M.-C. & Lee, M.-H. Oncogenic signals of HER-2/neu in regulating the stability of the cyclin-dependent kinase inhibitor p27. J. Biol. Chem. 275, 24735–24739 (2000).
  Article CAS PubMed Google Scholar
• Donovan, J.C., Milic, A. & Slingerland, J.M. Constitutive MEK/MAPK activation leads to p27Kip1 deregulation and antiestrogen resistance in human breast cancer cells. J. Biol. Chem. 276, 40888–40895 (2001).
  Article CAS PubMed Google Scholar
• Coffer, P.J., Jin, J. & Woodgett, J.R. Protein kinase B (c-Akt): A multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem. J. 335, 1–13 (1998).
  Article CAS PubMed PubMed Central Google Scholar
• Stampfer, M. Isolation and growth of human mammary epithelial cells. J. Tissue Cult. Methods 9, 107–115 (1985).
  Article Google Scholar
• Soule, H.D., Vazguez, J., Long, A., Albert, S. & Brennan, M. A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51, 1409–1416 (1973).
  Article CAS PubMed Google Scholar
• Herlyn, M. Human melanoma: Development and progression. Cancer Metastasis Rev. 9, 101–112 (1990).
  Article CAS PubMed Google Scholar
• Adam, S.A., Sterne-Marr, R. & Gerace, L. Nuclear protein import using digitonin-permeabilized cells. Methods Enzymol. 97–110 (1992).
• Franke, T.F. et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81, 727–736 (1995).
  Article CAS PubMed Google Scholar