Isolation of deletion alleles by G4 DNA-induced mutagenesis (original) (raw)

Nature Methods volume 6, pages 655–657 (2009)Cite this article

Abstract

Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G3NxG3NxG3NxG3, a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.

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Acknowledgements

We thank members of the Tijsterman lab for discussions, R. Plasterk for generous support and WormBase curators for building the platform that allows C. elegans genomic research. This work was funded by a Horizon grant from the Netherlands Genomics Initiative and a Vidi grant from ZonMw to M.T.

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Author notes

  1. Daphne B Pontier, Evelien Kruisselbrink & Marcel Tijsterman
    Present address: Present address: Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands.,

Authors and Affiliations

  1. Hubrecht Institute–Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands
    Daphne B Pontier, Evelien Kruisselbrink, Victor Guryev & Marcel Tijsterman

Authors

  1. Daphne B Pontier
  2. Evelien Kruisselbrink
  3. Victor Guryev
  4. Marcel Tijsterman

Contributions

D.B.P., E.K. and M.T. performed the experiments and analyzed the data. V.G. performed the bioinformatics analysis. M.T. conceived and supervised the project, and wrote the paper.

Corresponding author

Correspondence toMarcel Tijsterman.

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Pontier, D., Kruisselbrink, E., Guryev, V. et al. Isolation of deletion alleles by G4 DNA-induced mutagenesis.Nat Methods 6, 655–657 (2009). https://doi.org/10.1038/nmeth.1362

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