Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells (original) (raw)

• Nicholls, D.G. & Locke, R.M. Thermogenic mechanisms in brown fat. Physiol. Rev. 64, 1–64 (1984).
  Article CAS Google Scholar
• Block, B.A. Thermogenesis in muscle. Annu. Rev. Physiol. 56, 535–577 (1994).
  Article CAS Google Scholar
• Silva, J.E. Thermogenic mechanisms and their hormonal regulation. Physiol. Rev. 86, 435–464 (2006).
  Article CAS Google Scholar
• Enerbäck, S. et al. Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. Nature 387, 90–94 (1997).
  Article Google Scholar
• Matthias, A. et al. Thermogenic responses in brown fat cells are fully UCP1-dependent. J. Biol. Chem. 275, 25073–25081 (2000).
  Article CAS Google Scholar
• Cannon, B. & Nedergaard, J. Brown adipose tissue: function and physiological significance. Physiol. Rev. 84, 277–359 (2004).
  Article CAS Google Scholar
• de Meis, L. Uncoupled ATPase activity and heat production by the sarcoplasmic reticulum Ca2+-ATPase. Regulation by ATP. J. Biol. Chem. 276, 25078–25087 (2001).
  Article CAS Google Scholar
• Meis, L., Arruda, A.P. & Carvalho, D.P. Role of sarco/endoplasmic reticulum Ca2+-ATPase in thermogenesis. Biosci. Rep. 25, 181–190 (2005).
  Article CAS Google Scholar
• Paulik, M.A. et al. Development of infrared imaging to measure thermogenesis in cell culture: thermogenic effects of uncoupling protein-2 troglitazone, and β-adrenoceptor agonists. Pharm. Res. 15, 944–949 (1998).
  Article CAS Google Scholar
• Schröder, H.J. & Power, G.G. Engine and radiator: fetal and placental interactions for heat dissipation. Exp. Physiol. 82, 403–414 (1997).
  Article Google Scholar
• Suzuki, M., Tseeb, V., Oyama, K. & Ishiwata, S. Microscopic detection of thermogenesis in a single HeLa cells. Biophys. J. 92, L46–L48 (2007).
  Article CAS Google Scholar
• Gota, C., Okabe, K., Funatsu, T., Harada, Y. & Uchiyama, S. Hydrophilic fluorescent nanogel thermometer for intracellular thermometry. J. Am. Chem. Soc. 131, 2766–2767 (2009).
  Article CAS Google Scholar
• Yang, J.-M., Yang, H. & Lin, L. Quantum dot nano thermometers reveal heterogeneous local thermogenesis in living cells. ACS Nano 5, 5067–5071 (2011).
  Article CAS Google Scholar
• Oyama, K. et al. Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells. Lab Chip 12, 1591–1593 (2012).
  Article CAS Google Scholar
• Okabe, K. et al. Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy. Nat. Commun. 3, 705 (2012).
  Article Google Scholar
• Kucsko, G. et al. Nanometre-scale thermometry in a living cell. Nature 500, 54–58 (2013).
  Article CAS Google Scholar
• Tsien, R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509–544 (1998).
  Article CAS Google Scholar
• Chudakov, D.M., Lukyanov, S. & Lukyanov, K.A. Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol. 23, 605–613 (2005).
  Article CAS Google Scholar
• Hurme, R., Berndt, K.D., Normark, S.J. & Rhen, M. A proteinaceous gene regulatory thermometer in Salmonella. Cell 90, 55–64 (1997).
  Article CAS Google Scholar
• Naik, R.R., Kirkpatrick, S.M. & Stone, M.O. The thermostability of an α-helical coiled-coil protein and its potential use in sensor applications. Biosens. Bioelectron. 16, 1051–1057 (2001).
  Article CAS Google Scholar
• De Angelis, D.A., Miesenböck, G., Zemelman, B.V. & Rothman, J.E. PRIM: proximity imaging of green fluorescent protein-tagged polypeptides. Proc. Natl. Acad. Sci. USA 95, 12312–12316 (1998).
  Article CAS Google Scholar
• VanEngelenburg, S.B. & Palmer, A.E. Fluorescent biosensors of protein function. Curr. Opin. Chem. Biol. 12, 60–65 (2008).
  Article CAS Google Scholar
• Terada, H. The interaction of highly active uncouplers with mitochondria. Biochim. Biophys. Acta 639, 225–242 (1981).
  Article CAS Google Scholar
• Ahmad-Junan, S.A., Amos, P.C., Cockerill, G.S., Levett, P.C. & Whiting, D.A. Synthetic studies on electron transport inhibitors related to natural products. Biochem. Soc. Trans. 22, 237–241 (1994).
  Article CAS Google Scholar
• Smiley, S.T. et al. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. USA 88, 3671–3675 (1991).
  Article CAS Google Scholar
• Imamura, H. et al. Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators. Proc. Natl. Acad. Sci. USA 106, 15651–15656 (2009).
  Article CAS Google Scholar
• Cannon, B. & Nedergaard, J. Nonshivering thermogenesis and its adequate measurement in metabolic studies. J. Exp. Biol. 214, 242–253 (2011).
  Article Google Scholar
• Arruda, A.P. et al. Cold tolerance in hypothyroid rabbits: role of skeletal muscle mitochondria and sarcoplasmic reticulum Ca2+ ATPase isoform 1 heat production. Endocrinology 149, 6262–6271 (2008).
  Article CAS Google Scholar
• Lupas, A. Coiled coils: new structures and new functions. Trends Biochem. Sci. 21, 375–382 (1996).
  Article CAS Google Scholar
• Patterson, G.H., Knobel, S.M., Sharif, W.D., Kain, S.R. & Piston, D.W. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys. J. 73, 2782–2790 (1997).
  Article CAS Google Scholar
• Azzu, V. & Brand, M.D. The on-off switches of the mitochondrial uncoupling proteins. Trends Biochem. Sci. 35, 298–307 (2010).
  Article CAS Google Scholar
• Mall, S. et al. The presence of sarcolipin results in increased heat production by Ca2+-ATPase. J. Biol. Chem. 281, 36597–36602 (2006).
  Article CAS Google Scholar
• Bal, N.C. et al. Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat. Med. 18, 1575–1579 (2012).
  Article CAS Google Scholar
• Collins, T.J., Berridge, M.J., Lipp, P. & Bootman, M.D. Mitochondria are morphologically and functionally heterogenous with in cells. EMBO J. 21, 1616–1627 (2002).
  Article CAS Google Scholar
• Kuznetsov, A.V. & Margreiter, R. Heterogeneity of mitochondria and mitochondrial function within cells as another level of mitochondrial complexity. Int. J. Mol. Sci. 10, 1911–1929 (2009).
  Article CAS Google Scholar
• Voets, T., Talavera, K., Owsianik, G. & Nilius, B. Sensing with TRP channels. Nat. Chem. Biol. 1, 85–92 (2005).
  Article CAS Google Scholar
• Montell, C. & Caterina, M.J. Thermoregulation: channels that are cool to the core. Curr. Biol. 17, R885–R887 (2007).
  Article CAS Google Scholar
• Haneda, T., Okada, N., Nakazawa, N., Kawakami, T. & Danbara, H. Complete DNA sequence and comparative analysis of the 50-kilobase virulence plasmid of Salmonella enterica serovar choleraesuis. Infect. Immun. 69, 2612–2620 (2001).
  Article CAS Google Scholar
• Dayel, M.J., Hom, E.F.Y. & Verkman, A.S. Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum. Biophys. J. 76, 2843–2851 (1999).
  Article CAS Google Scholar
• Wang, Y. & Weiner, H. The presequence of rat liver aldehyde dehydrogenase requires the presence of an α-helix at its N-terminal region which is stabilized by the helix at its C temini. J. Biol. Chem. 268, 4759–4765 (1993).
  CAS PubMed Google Scholar
• Schneider, J.P., Lear, J.D. & DeGrado, W.F. A designed buried salt bridge in a heterodimeric coiled-coil. J. Am. Chem. Soc. 119, 5742–5743 (1997).
  Article CAS Google Scholar
• Omatsu-Kanbe, M. & Matsuura, H. Inhibition of store-operated Ca2+ entry by extracellular ATP in rat brown adipocytes. J. Physiol. (Lond.) 521, 601–615 (1999).
  Article CAS Google Scholar
• Luo, J. et al. A protocol for rapid generation of recombinant adenoviruses using the AdEasy system. Nat. Protoc. 2, 1236–1247 (2007).
  Article CAS Google Scholar
• Grassi, F., Fucile, S. & Eusebi, F. Ca2+ signaling pathways activated by acetylcholine in mouse C2C12 myotubes. Pflugers Arch. 428, 340–345 (1994).
  Article CAS Google Scholar