Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny (original) (raw)

• Barker, N. et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449, 1003–1007 (2007).
  Article CAS PubMed Google Scholar
• Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262–265 (2009).
  Article CAS PubMed Google Scholar
• Sato, T. et al. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469, 415–418 (2011).
  Article CAS PubMed Google Scholar
• Yilmaz, Ö.H. et al. mTORC1 in the Paneth cell niche couples intestinal stem-cell function to calorie intake. Nature 486, 490–495 (2012).
  Article CAS PubMed PubMed Central Google Scholar
• Farin, H.F., van Es, J.H. & Clevers, H. Redundant sources of Wnt regulate intestinal stem cells and promote formation of Paneth cells. Gastroenterology 143, 1518–1529 (2012).
  Article CAS PubMed Google Scholar
• Snippert, H.J. et al. Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell 143, 134–144 (2010).
  Article CAS PubMed Google Scholar
• Scoville, D.H., Sato, T., He, X.C. & Li, L. Current view: intestinal stem cells and signaling. Gastroenterology 134, 849–864 (2008).
  Article CAS PubMed Google Scholar
• van der Flier, L.G. & Clevers, H. Stem cells, self-renewal, and differentiation in the intestinal epithelium. Annu. Rev. Physiol. 71, 241–260 (2009).
  Article CAS PubMed Google Scholar
• Crosnier, C., Stamataki, D. & Lewis, J. Organizing cell renewal in the intestine: stem cells, signals and combinatorial control. Nat. Rev. Genet. 7, 349–359 (2006).
  Article CAS PubMed Google Scholar
• Stanger, B.Z., Datar, R., Murtaugh, L.C. & Melton, D.A. Direct regulation of intestinal fate by Notch. Proc. Natl. Acad. Sci. USA 102, 12443–12448 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Zecchini, V., Domaschenz, R., Winton, D. & Jones, P. Notch signaling regulates the differentiation of post-mitotic intestinal epithelial cells. Genes Dev. 19, 1686–1691 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Powell, D.W., Pinchuk, I.V., Saada, J.I., Chen, X. & Mifflin, R.C. Mesenchymal cells of the intestinal lamina propria. Annu. Rev. Physiol. 73, 213–237 (2011).
  Article CAS PubMed PubMed Central Google Scholar
• Greenblatt, D.Y. et al. Valproic acid activates Notch-1 signaling and regulates the neuroendocrine phenotype in carcinoid cancer cells. Oncologist 12, 942–951 (2007).
  Article CAS PubMed Google Scholar
• Stockhausen, M.T., Sjölund, J., Manetopoulos, C. & Axelson, H. Effects of the histone deacetylase inhibitor valproic acid on Notch signalling in human neuroblastoma cells. Br. J. Cancer 92, 751–759 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• van der Flier, L.G. et al. Transcription factor achaete scute-like 2 controls intestinal stem cell fate. Cell 136, 903–912 (2009).
  Article CAS PubMed Google Scholar
• de Lau, W. et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476, 293–297 (2011).
  CAS PubMed Google Scholar
• Wang, F. et al. Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay. Gastroenterology 145, 383–395 (2013).
  Article CAS PubMed Google Scholar
• van Es, J.H. et al. Dll1+ secretory progenitor cells revert to stem cells upon crypt damage. Nat. Cell Biol. 14, 1099–1104 (2012).
  Article CAS PubMed PubMed Central Google Scholar
• Sato, T. et al. Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology 141, 1762–1772 (2011).
  Article CAS PubMed Google Scholar
• Jung, P. et al. Isolation and in vitro expansion of human colonic stem cells. Nat. Med. 17, 1225–1227 (2011).
  Article CAS PubMed Google Scholar
• Subramanian, A. et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc. Natl. Acad. Sci. USA 102, 15545–15550 (2005).
  CAS PubMed PubMed Central Google Scholar
• Muñoz, J. et al. The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers. EMBO J. 31, 3079–3091 (2012).
  Article PubMed PubMed Central Google Scholar
• Yu, D., Cozma, D., Park, A. & Thomas-Tikhonenko, A. Functional validation of genes implicated in lymphomagenesis: an in vivo selection assay using a Myc-induced B-cell tumor. Ann. NY Acad. Sci. 1059, 145–159 (2005).
  Article CAS PubMed Google Scholar
• Milano, J. et al. Modulation of notch processing by γ-secretase inhibitors causes intestinal goblet cell metaplasia and induction of genes known to specify gut secretory lineage differentiation. Toxicol. Sci. 82, 341–358 (2004).
  Article CAS PubMed Google Scholar
• Wong, G.T. et al. Chronic treatment with the γ-secretase inhibitor LY-411,575 inhibits β-amyloid peptide production and alters lymphopoiesis and intestinal cell differentiation. J. Biol. Chem. 279, 12876–12882 (2004).
  Article CAS PubMed Google Scholar
• van Es, J.H. et al. Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells. Nature 435, 959–963 (2005).
  Article CAS PubMed Google Scholar
• Fre, S. et al. Notch signals control the fate of immature progenitor cells in the intestine. Nature 435, 964–968 (2005).
  Article CAS PubMed Google Scholar
• Bain, J. et al. The selectivity of protein kinase inhibitors: a further update. Biochem. J. 408, 297–315 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Conti, L. et al. Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. 3, e283 (2005).
  Article PubMed PubMed Central Google Scholar
• Pellegrinet, L. et al. Dll1- and Dll4-mediated notch signaling are required for homeostasis of intestinal stem cells. Gastroenterology 140, 1230–1240 (2011).
  Article CAS PubMed Google Scholar
• Riccio, O. et al. Loss of intestinal crypt progenitor cells owing to inactivation of both Notch1 and Notch2 is accompanied by derepression of CDK inhibitors p27Kip1 and p57Kip2. EMBO Rep. 9, 377–383 (2008).
  Article CAS PubMed PubMed Central Google Scholar
• Kazanjian, A., Noah, T., Brown, D., Burkart, J. & Shroyer, N.F. Atonal homolog 1 is required for growth and differentiation effects of notch/gamma-secretase inhibitors on normal and cancerous intestinal epithelial cells. Gastroenterology 139, 918–928 (2010).
  Article CAS PubMed Google Scholar
• Kim, T.H. & Shivdasani, R.A. Genetic evidence that intestinal Notch functions vary regionally and operate through a common mechanism of Math1 repression. J. Biol. Chem. 286, 11427–11433 (2011).
  Article CAS PubMed PubMed Central Google Scholar
• VanDussen, K.L. et al. Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells. Development 139, 488–497 (2012).
  Article CAS PubMed PubMed Central Google Scholar
• D'Souza, B., Miyamoto, A. & Weinmaster, G. The many facets of Notch ligands. Oncogene 27, 5148–5167 (2008).
  Article CAS PubMed PubMed Central Google Scholar
• Shroyer, N.F. et al. Intestine-specific ablation of mouse atonal homolog 1 (Math1) reveals a role in cellular homeostasis. Gastroenterology 132, 2478–2488 (2007).
  Article CAS PubMed Google Scholar
• Han, H. et al. Inducible gene knockout of transcription factor recombination signal binding protein-J reveals its essential role in T versus B lineage decision. Int. Immunol. 14, 637–645 (2002).
  Article CAS PubMed Google Scholar
• Shibata, H. et al. Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene. Science 278, 120–123 (1997).
  Article CAS PubMed Google Scholar
• Barker, N. et al. Lgr5+ve stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell 6, 25–36 (2010).
  CAS PubMed Google Scholar