Use of quantum dots for live cell imaging (original) (raw)

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• Published: October 2004

Nature Methods volume 1, pages 73–78 (2004)Cite this article

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• Quantum dots (QDs; e.g., Quantum Dot Corporation or Evident Technology)

• Cells or tissue for labeling, prepared appropriately depending on the application (QDs can be used to tag live cells, label cell-surface proteins, or label fixed cells or tissue sections)

• Amylose resin (New England Biolabs)

• Antibodies of interest

• Avidin (Sigma Chemicals)

• Bovine serum albumin (BSA), 1% in PBS

• Lipid-based transfection reagent (e.g., Lipofectamine 2000 (Invitrogen) or Fugene 6 (Roche))

• Maltose (Sigma Chemicals)

• Maltose-binding protein fused to the basic leucine zipper domain (MBP-zb) and protein G fused to the basic leucine zipper domain (PG-zb) expressed and purified from bacteria as described elsewhere7.

• Phosphate-buffered saline (PBS; Sigma Chemicals)

• Sodium tetraborate (Sigma Chemicals)

• Sulfo-NHS-SS biotin (Pierce Biotechnology)

• Tris-buffered saline (TBS; Sigma Chemicals)

Quantum dots (QDs; e.g., Quantum Dot Corporation or Evident Technology)

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Author information

Authors and Affiliations

1. The Rockefeller University, Laboratory of Cellular Biophysics, 1230 York Avenue, New York, 10021, New York, USA
   Jyoti K Jaiswal & Sanford M Simon
2. Center for Bio/Molecular Science and Engineering, United States Naval Research Laboratory, Washington, 20375, DC, USA
   Ellen R Goldman
3. Division of Optical Sciences, United States Naval Research Laboratory, Washington, 20375, DC, USA
   Hedi Mattoussi

Authors

1. Jyoti K Jaiswal
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2. Ellen R Goldman
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3. Hedi Mattoussi
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4. Sanford M Simon
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Jaiswal, J., Goldman, E., Mattoussi, H. et al. Use of quantum dots for live cell imaging.Nat Methods 1, 73–78 (2004). https://doi.org/10.1038/nmeth1004-73

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• Issue Date: October 2004
• DOI: https://doi.org/10.1038/nmeth1004-73

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