Cocking, E.C. A method for the isolation of plant protoplasts and vacuoles. Nature187, 927–929 (1960). Article Google Scholar
Jacobsen, J.V. & Beach, L.R. Control of transcription of α-amylase and rRNA genes in barley aleurone protoplasts by gibberellin and abscisic acid. Nature316, 275–277 (1985). ArticleCAS Google Scholar
Assmann, S.M., Simoncini, L. & Schroeder, J.I. Blue light activates electrogenic ion pumping in guard cell protoplasts of Vicia faba . Nature318, 285–287 (1985). ArticleCAS Google Scholar
Kovtun, Y., Chiu, W.L., Tena, G. & Sheen, J. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc. Natl. Acad. Sci. USA97, 2940–2945 (2000). ArticleCAS Google Scholar
Sheen, J. Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J.12, 3497–3505 (1993). ArticleCASPubMed Central Google Scholar
Luehrsen, K.R., de Wet, J.R. & Walbot, V. Transient expression analysis in plants using firefly luciferase reporter gene. Methods Enzymol.216, 397–414 (1992). ArticleCAS Google Scholar
Norris, S.R., Meyer, S.E. & Callis, J. The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression. Plant Mol. Biol.21, 895–906 (1993). ArticleCAS Google Scholar
Worley, C.K. et al. Degradation of Aux/IAA proteins is essential for normal auxin signalling. Plant J.21, 553–563 (2000). ArticleCAS Google Scholar
Tao, L., Cheung, A.Y., Nibau, C. & Wu, H. RAC GTPases in tobacco and Arabidopsis mediate auxin-induced formation of proteolytically active nuclear protein bodies that contain AUX/IAA proteins. Plant Cell17, 2369–2383 (2005). ArticleCASPubMed Central Google Scholar
Wang, S., Tiwari, S.B., Hagen, G. & Guilfoyle, T.J. Auxin response factor7 restores the expression of auxin-responsive genes in mutant Arabidopsis leaf mesophyll protoplasts. Plant Cell17, 1979–1993 (2005). ArticleCASPubMed Central Google Scholar
Yanagisawa, S., Yoo, S.-D. & Sheen, J. Differential regulation of EIN3 stability by glucose and ethylene signalling in plants. Nature425, 521–525 (2003). ArticleCAS Google Scholar
Hwang, I. & Sheen, J. Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature413, 383–389 (2001). ArticleCAS Google Scholar
Asai, T. et al. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature415, 977–983 (2002). ArticleCAS Google Scholar
He, P. et al. Specific bacterial suppressors of MAMP signalling upstream of MAPKKK in Arabidopsis innate immunity. Cell125, 563–575 (2006). ArticleCAS Google Scholar
Cho, Y.-H., Yoo, S.-D. & Sheen, J. Regulatory functions of nuclear hexokinase1 complex in glucose signalling. Cell127, 579–589 (2006). ArticleCAS Google Scholar
Ehlert, A. et al. Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. Plant J.46, 890–900 (2006). ArticleCAS Google Scholar
Chiu, W. et al. Engineered GFP as a vital reporter in plants. Curr. Biol.6, 325–330 (1996). ArticleCAS Google Scholar
Lee, Y., Kim, D., Kim, Y. & Hwang, I. Identification of a signal that distinguishes between the chloroplast outer envelope membrane and the endomembrane system in vivo . Plant Cell13, 2175–2190 (2001). ArticleCASPubMed Central Google Scholar
Jin, J.B et al. A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis . Plant Cell13, 1511–1526 (2001). ArticleCASPubMed Central Google Scholar
Sohn, E.J. et al. Rha1, an Arabidopsis Rab5 homolog, plays a critical role in the vacuolar trafficking of soluble cargo proteins. Plant Cell15, 1057–1070 (2003). ArticleCASPubMed Central Google Scholar
Kim, H., Park, M., Kim, S. & Hwang, I. Actin filaments play a critical role in vacuolar trafficking at the Golgi complex in plant cells. Plant Cell17, 888–902 (2005). ArticleCASPubMed Central Google Scholar
Birnbaum, K. et al. A gene expression map of the Arabidopsis root. Science302, 1956–1960 (2003). ArticleCAS Google Scholar
Boudsocq, M., Barbier-Brygoo, H. & Lauriere, C. Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana . J. Biol. Chem.279, 41758–41766 (2004). ArticleCAS Google Scholar
Picard, D., Salser, S.J. & Yamamoto, K.R. A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptor. Cell54, 1073–1080 (1988). ArticleCAS Google Scholar
Asai, T. et al. Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways. Plant Cell12, 1823–1835 (2000). ArticleCASPubMed Central Google Scholar
Wenck, A.R. & Marton, L. Large-scale protoplast isolation and regeneration of Arabidopsis thaliana . Biotechniques18, 640–643 (1995). CASPubMed Google Scholar
Damm, B., Schmidt, R. & Willmitzer, L. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts. Mol. Gen. Genet.217, 6–12 (1989). ArticleCAS Google Scholar
Masson, J. & Paszkowski, J. Culture response of Arabidopsis thaliana protoplasts is determined by growth conditions of donor plants. Plant J.2, 829–833 (1992). Article Google Scholar