Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification (original) (raw)
References
Bartel, D.P. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell116, 281–297 (2004). ArticleCAS Google Scholar
Wienholds, E. & Plasterk, R.H. MicroRNA function in animal development. FEBS Lett.579, 5911–5922 (2005). ArticleCAS Google Scholar
Kloosterman, W.P. & Plasterk, R.H. The diverse functions of microRNAs in animal development and disease. Dev. Cell.11, 441–450 (2006). ArticleCASPubMed Central Google Scholar
Brennecke, J., Hipfner, D.R., Stark, A., Russel, R.B. & Cohen, S.M. Bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell113, 25–36 (2003). ArticleCAS Google Scholar
Mansfield, J.H. et al. MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat. Genet.36, 1079–1083 (2004). ArticleCASPubMed Central Google Scholar
Koshkin, A.A. et al. LNA (locked nucleic acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition. Tetrahedron54, 3607–3630 (1998). ArticleCAS Google Scholar
Braasch, D.A. & Corey, D.R. Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA. Chem. Biol.8, 1–7 (2001). ArticleCASPubMed Central Google Scholar
Kurreck, J., Wyszko, E., Gillen, C. & Erdmann, V.A. Design of antisense oligonucleotides stabilized by locked nucleic acids. Nucleic Acids Res.30, 1911–1918 (2002). ArticleCASPubMed Central Google Scholar
Jacobsen, N. et al. Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture. Nucleic Acids Res.32, e64 (2004). ArticlePubMed Central Google Scholar
Kauppinen, S., Vester, B. & Wengel, J. Locked nucleic acid: high-affinity targeting of complementary RNA for RNomics. Handb. Exp. Pharmacol.173, 405–422 (2006). ArticleCAS Google Scholar
Wienholds, E. et al. MicroRNA expression in zebrafish embryonic development. Science309, 310–311 (2005). ArticleCAS Google Scholar
Kloosterman, W.P., Wienholds, E., de Bruijn, E., Kauppinen, S. & Plasterk, R.H. In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat. Methods3, 27–29 (2006). ArticleCAS Google Scholar
Wheeler, G., Ntounia-Fousara, S., Granda, B., Rathjen, T. & Dalmay, T. Identification of new central nervous system specific mouse microRNAs. FEBS Lett.580, 2195–2200 (2006). ArticleCASPubMed Central Google Scholar
Nelson, P.T. et al. RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain. RNA12, 187–191 (2006). ArticleCASPubMed Central Google Scholar
Obernosterer, G., Leuschner, P.J., Alenius, M. & Martinez, J. Post-transcriptional regulation of microRNA expression. RNA12, 1161–1167 (2006). ArticleCASPubMed Central Google Scholar
Obernosterer, G., Martinez, J. & Alenius, M. Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections. Nat. Protoc.2, 1508–1514 (2007). ArticleCASPubMed Central Google Scholar
Silahtaroglu, A.N., Tommerup, N. & Vissing, H. FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers. Mol. Cell. Probes17, 165–169 (2003). ArticleCASPubMed Central Google Scholar
Silahtaroglu, A., Pfundheller, H., Koshkin, A., Tommerup, N. & Kauppinen, S. LNA-modified oligonucleotides are highly efficient as FISH probes. Cytogenet. Genome Res.107, 32–37 (2004). ArticleCASPubMed Central Google Scholar
Christoffersen, N.R., Silahtaroglu, A., Orom, U.A., Kauppinen, S. & Lund, A.H. miR-200b mediates post-transcriptional repression of ZFHX1B. RNA13, 1172–1178 (2007). ArticleCASPubMed Central Google Scholar
Kerstens, H.M., Poddighe, P.J. & Hanselaar, A.G. A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramide. J. Histochem. Cytochem.43, 347–352 (1995). ArticleCASPubMed Central Google Scholar