- Roeder, R.G. Transcriptional regulation and the role of diverse coactivators in animal cells. FEBS Lett. 579, 909–915 (2005).
Article CAS Google Scholar
- Malik, S. & Roeder, R.G. The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation. Nat. Rev. Genet. 11, 761–772 (2010).
Article CAS Google Scholar
- Baek, H.J., Malik, S., Qin, J. & Roeder, R.G. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s. Mol. Cell. Biol. 22, 2842–2852 (2002).
Article CAS Google Scholar
- Malik, S., Baek, H.J., Wu, W. & Roeder, R.G. Structural and functional characterization of PC2 and RNA polymerase II-associated subpopulations of metazoan Mediator. Mol. Cell. Biol. 25, 2117–2129 (2005).
Article CAS Google Scholar
- Mittler, G., Kremmer, E., Timmers, H.T. & Meisterernst, M. Novel critical role of a human Mediator complex for basal RNA polymerase II transcription. EMBO Rep. 2, 808–813 (2001).
Article CAS Google Scholar
- Poss, Z.C., Ebmeier, C.C. & Taatjes, D.J. The Mediator complex and transcription regulation. Crit. Rev. Biochem. Mol. Biol. 48, 575–608 (2013).
Article CAS Google Scholar
- Black, J.C., Choi, J.E., Lombardo, S.R. & Carey, M. A mechanism for coordinating chromatin modification and preinitiation complex assembly. Mol. Cell 23, 809–818 (2006).
Article CAS Google Scholar
- Wallberg, A.E., Yamamura, S., Malik, S., Spiegelman, B.M. & Roeder, R.G. Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1α. Mol. Cell 12, 1137–1149 (2003).
Article CAS Google Scholar
- Lin, J.J. et al. Mediator coordinates PIC assembly with recruitment of CHD1. Genes Dev. 25, 2198–2209 (2011).
Article CAS Google Scholar
- Malik, S., Barrero, M.J. & Jones, T. Identification of a regulator of transcription elongation as an accessory factor for the human Mediator coactivator. Proc. Natl. Acad. Sci. USA 104, 6182–6187 (2007).
Article CAS Google Scholar
- Nock, A., Ascano, J.M., Barrero, M.J. & Malik, S. Mediator-regulated transcription through the +1 nucleosome. Mol. Cell 48, 837–848 (2012).
Article CAS Google Scholar
- Takahashi, H. et al. Human mediator subunit MED26 functions as a docking site for transcription elongation factors. Cell 146, 92–104 (2011).
Article CAS Google Scholar
- Lai, F. et al. Activating RNAs associate with Mediator to enhance chromatin architecture and transcription. Nature 494, 497–501 (2013).
Article CAS Google Scholar
- Kagey, M.H. et al. Mediator and cohesin connect gene expression and chromatin architecture. Nature 467, 430–435 (2010).
Article CAS Google Scholar
- Eyboulet, F. et al. Mediator links transcription and DNA repair by facilitating Rad2/XPG recruitment. Genes Dev. 27, 2549–2562 (2013).
Article CAS Google Scholar
- Schiano, C. et al. Involvement of Mediator complex in malignancy. Biochim. Biophys. Acta 1845, 66–83 (2014).
CAS PubMed Google Scholar
- Spaeth, J.M., Kim, N.H. & Boyer, T.G. Mediator and human disease. Semin. Cell Dev. Biol. 22, 776–787 (2011).
Article CAS Google Scholar
- Bourbon, H.M. Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex. Nucleic Acids Res. 36, 3993–4008 (2008).
Article CAS Google Scholar
- Blazek, E., Mittler, G. & Meisterernst, M. The mediator of RNA polymerase II. Chromosoma 113, 399–408 (2005).
Article CAS Google Scholar
- Baumli, S., Hoeppner, S. & Cramer, P. A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer. J. Biol. Chem. 280, 18171–18178 (2005).
Article CAS Google Scholar
- Koschubs, T. et al. Identification, structure, and functional requirement of the Mediator submodule Med7N/31. EMBO J. 28, 69–80 (2009).
Article CAS Google Scholar
- Larivière, L. et al. Structure of the Mediator head module. Nature 492, 448–451 (2012).
Article Google Scholar
- Imasaki, T. et al. Architecture of the Mediator head module. Nature 475, 240–243 (2011).
Article CAS Google Scholar
- Robinson, P.J., Bushnell, D.A., Trnka, M.J., Burlingame, A.L. & Kornberg, R.D. Structure of the mediator head module bound to the carboxy-terminal domain of RNA polymerase II. Proc. Natl. Acad. Sci. USA 109, 17931–17935 (2012).
Article CAS Google Scholar
- Larivière, L. et al. Model of the Mediator middle module based on protein cross-linking. Nucleic Acids Res. 41, 9266–9273 (2013).
Article Google Scholar
- Guglielmi, B. et al. A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res. 32, 5379–5391 (2004).
Article CAS Google Scholar
- Tsai, K.L. et al. Subunit architecture and functional modular rearrangements of the transcriptional mediator complex. Cell 157, 1430–1444 (2014).
Article CAS Google Scholar
- Wang, X. et al. Redefining the modular organization of the core Mediator complex. Cell Res. 24, 796–808 (2014).
Article CAS Google Scholar
- Malik, S., Gu, W., Wu, W., Qin, J. & Roeder, R.G. The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs. Mol. Cell 5, 753–760 (2000).
Article CAS Google Scholar
- Berger, I., Fitzgerald, D.J. & Richmond, T.J. Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol. 22, 1583–1587 (2004).
Article CAS Google Scholar
- Ge, K. et al. Transcription coactivator TRAP220 is required for PPARã2-stimulated adipogenesis. Nature 417, 563–567 (2002).
Article CAS Google Scholar
- Nonet, M.L. & Young, R.A. Intragenic and extragenic suppressors of mutations in the heptapeptide repeat domain of Saccharomyces cerevisiae RNA polymerase II. Genetics 123, 715–724 (1989).
CAS PubMed PubMed Central Google Scholar
- Thompson, C.M., Koleske, A.J., Chao, D.M. & Young, R.A. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73, 1361–1375 (1993).
Article CAS Google Scholar
- Malik, S. & Roeder, R.G. Isolation and functional characterization of the TRAP/mediator complex. Methods Enzymol. 364, 257–284 (2003).
Article CAS Google Scholar
- Dotson, M.R. et al. Structural organization of yeast and mammalian mediator complexes. Proc. Natl. Acad. Sci. USA 97, 14307–14310 (2000).
Article CAS Google Scholar
- Sato, S. et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell 14, 685–691 (2004).
Article CAS Google Scholar
- Conaway, R.C. & Conaway, J.W. The Mediator complex and transcription elongation. Biochim. Biophys. Acta 1829, 69–75 (2013).
Article CAS Google Scholar
- Ito, M. et al. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. Mol. Cell 3, 361–370 (1999).
Article CAS Google Scholar
- Yuan, C.X., Ito, M., Fondell, J.D., Fu, Z.Y. & Roeder, R.G. The TRAP220 component of a thyroid hormone receptor-associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion. Proc. Natl. Acad. Sci. USA 95, 7939–7944 (1998).
Article CAS Google Scholar
- Larivière, L. et al. Structure and TBP binding of the Mediator head subcomplex Med8–Med18–Med20. Nat. Struct. Mol. Biol. 13, 895–901 (2006).
Article Google Scholar
- Cai, G. et al. Interaction of the mediator head module with RNA polymerase II. Structure 20, 899–910 (2012).
Article CAS Google Scholar
- Baek, H.J., Kang, Y.K. & Roeder, R.G. Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly. J. Biol. Chem. 281, 15172–15181 (2006).
Article CAS Google Scholar
- Leitner, A. et al. Probing native protein structures by chemical cross-linking, mass spectrometry, and bioinformatics. Mol. Cell. Proteomics 9, 1634–1649 (2010).
Article CAS Google Scholar
- Soutourina, J., Wydau, S., Ambroise, Y., Boschiero, C. & Werner, M. Direct interaction of RNA polymerase II and mediator required for transcription in vivo. Science 331, 1451–1454 (2011).
Article CAS Google Scholar
- Tsai, K.L. et al. A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction. Nat. Struct. Mol. Biol. 20, 611–619 (2013).
Article CAS Google Scholar
- Näär, A.M., Taatjes, D.J., Zhai, W., Nogales, E. & Tjian, R. Human CRSP interacts with RNA polymerase II CTD and adopts a specific CTD-bound conformation. Genes Dev. 16, 1339–1344 (2002).
Article Google Scholar
- Jishage, M. et al. Transcriptional regulation by Pol II(G) involving mediator and competitive interactions of Gdown1 and TFIIF with Pol II. Mol. Cell 45, 51–63 (2012).
Article CAS Google Scholar
- Lemaire, M., Xie, J., Meisterernst, M. & Collart, M.A. The NC2 repressor is dispensable in yeast mutated for the Sin4p component of the holoenzyme and plays roles similar to Mot1p in vivo. Mol. Microbiol. 36, 163–173 (2000).
Article CAS Google Scholar
- Marr, S.K., Lis, J.T., Treisman, J.E. & Marr, M.T. II The metazoan-specific Mediator Subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster. Mol. Cell. Biol. 34, 2710–2720 (2014).
Article Google Scholar
- Gu, W. et al. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. Mol. Cell 3, 97–108 (1999).
Article CAS Google Scholar
- Dignam, J.D., Lebovitz, R.M. & Roeder, R.G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475–1489 (1983).
Article CAS Google Scholar
- Malik, S., Wallberg, A.E., Kang, Y.K. & Roeder, R.G. TRAP/SMCC/mediator-dependent transcriptional activation from DNA and chromatin templates by orphan nuclear receptor hepatocyte nuclear factor 4. Mol. Cell. Biol. 22, 5626–5637 (2002).
Article CAS Google Scholar
- Shi, Y. et al. Structural characterization by cross-linking reveals the detailed architecture of a coatomer-related heptameric module from the nuclear pore complex. Mol. Cell. Proteomics 10.1074/mcp.M114.041673 (26 August 2014).
- Algret, R. et al. Molecular architecture and function of the SEA complex, a modulator of the TORC1 pathway. Mol. Cell. Proteomics 10.1074/mcp.M114.039388 (29 July 2014).
- Leitner, A. et al. Expanding the chemical cross-linking toolbox by the use of multiple proteases and enrichment by size exclusion chromatography. Mol. Cell. Proteomics 11 M111.014126 (2012).
- Yang, B. et al. Identification of cross-linked peptides from complex samples. Nat. Methods 9, 904–906 (2012).
Article CAS Google Scholar
- Qin, J. & Chait, B.T. Matrix-assisted laser desorption ion trap mass spectrometry: efficient isolation and effective fragmentation of peptide ions. Anal. Chem. 68, 2108–2112 (1996).
Article CAS Google Scholar
- Michalski, A., Neuhauser, N., Cox, J. & Mann, M. A systematic investigation into the nature of tryptic HCD spectra. J. Proteome Res. 11, 5479–5491 (2012).
Article CAS Google Scholar