Maternal Behavior Regulates Benzodiazepine/GABAA Receptor Subunit Expression in Brain Regions Associated with Fear in BALB/c and C57BL/6 Mice (original) (raw)

INTRODUCTION

Inbred strains of mice exhibit substantial differences in phenotype (Lindzey and Thiessen, 1970). The BALB/c and C57BL/6 mouse strains are among the most studied and reveal extensive behavioral differences (Oliverio et al, 1973; Peeler and Nowakowski, 1987; Belzung and Griebel, 2001), especially under the conditions of stress (Anisman et al, 2001). The more neophobic BALB/c strain exhibits greater evidence of fear-related behaviors in the open-field exploration (Oliverio et al, 1973; Griebel et al, 1993) and the light/dark choice tests (Beuzen and Belzung, 1995) as well as increased hypothalamic-pituitary-adrenal (HPA) responses to a wide range of stressors (Anisman et al, 2001). These variations in behavior are reflected in strain differences in neural systems relevant for the expression of fear, such as the benzodiazepine (BZ)/GABAA receptor complex. Compared with C57BL/6 animals, BALB/c mice show reduced BZ binding in receptor assays as measured using [3H]flunitrazepam or [3H]diazepam (Robertson, 1979; Chapoutier et al, 1991). Such differences in receptor density are associated with differences in BZ/GABAA receptor pharmacology. B-carboline-3-carboxylate (B-CCM), a benzodiazepine inverse agonist, results in a higher vulnerability to convulsions in BALB/c mice compared to C57BL/6 (Desforges et al, 1989). Moreover, diazepam-induced potentiation of GABA-mediated chloride influx is reduced in BALB/c compared to C57BL/6 mice (Mihic et al, 1992).

The results of an in vitro receptor autoradiography study using [3H]diazepam revealed a striking difference between C57 and BALB/c mice, with dramatically reduced BZ binding in the BALB/c animals that was largely restricted to the central nucleus of the amygdala (Hode et al, 2000), a region known to mediate fear-related behavior (for a review, see Rosen and Shulkin, 1998). The amygdala is also a critical site of action for the anxiolytic effects of BZ receptor agonists. Studies in humans support the idea that alterations in the GABAA/BZ receptor complex might form the basis of a vulnerability to anxiety disorders (for a review, see Gorman et al, 2000).

Not surprisingly, these findings have spawned efforts to identify the relevant genomic variation underlying the reliable differences in phenotype between BALB/c and C57 mice. However, recent findings from embryo-transfer experiments suggest a considerable epigenetic influence on the development of fear behavior in the BALB/c mice (Francis et al, 2002). Such influences may not be restricted to the prenatal period. There are considerable differences in the maternal behavior of BALB/c and C57BL/6 mice (Zaharia et al, 1996); C57BL/6 mothers lick/groom their pups more frequently than BALB/c mothers (Anisman et al, 2001). Crossfostering BALB/c pups onto C57BL/6 mothers shortly after birth markedly reduces behavioral differences between the strains (Zaharia et al, 1996). In the rat, differences in maternal licking/grooming are associated with individual differences in BZ/GABAA receptor subunit expression (Caldji et al, 2003) and fearfulness in response to novelty (Caldji et al, 1998). The adult offspring of high licking/grooming–arched-back nursing (LG-ABN) mothers show reduced behavioral responses to novelty and increased BZ receptor binding. Interestingly, the difference in BZ receptor binding is most pronounced in the amygdala (Caldji et al, 1998). Crossfostering the biological offspring of low-to-high LG-ABN mothers to high LG-ABN dams on the day of birth reverses the pattern of stress reactivity that is characteristic of the normal offspring of low LG-ABN mothers (Francis et al, 1999) as well as the differences in BZ/GABAA receptor binding (Caldji et al, 2003).

The effect of maternal care on the development of the BZ/GABAA receptor complex is associated with alterations in GABAA receptor subunit expression. The GABAA receptor complex in the rat forebrain, which often includes a ‘BZ’ binding site, is commonly arranged in a pentameric structure comprised of α, β, and γ subunits, in the form of two α, two β, and one γ subunit, or two α, one β, and two γ subunits (Barnard et al, 1988; Sieghart, 1995; McKernan and Whiting, 1997; Mehta and Ticku, 1999). The presence of a functional BZ site on the GABAA receptor complex is dependent on the presence of α and γ subunits. In the rat, maternal care significantly alters the expression of the _α_1 as well as both the _γ_1 and _γ_2 subunits in the amygdala, and the effects are reversed with crossfostering (Caldji et al, 2003). Taken together with the data on strain differences in maternal care, these findings suggest that differences in the BZ/GABAA receptor complex between BALB/c and C57 mice might emerge, at least in part, as a function of strain differences in mother–pup interactions during postnatal life, and therefore to a nongenomic mode of inheritance.

MATERIALS AND METHODS

Animals

Male/female BALB/cByJ and C57BL/6ByJ adult mice (Jackson Laboratories, Bar Harbor, ME) were maintained on a 12 : 12 light : dark schedule (lights on at 0800) with free access to food and water. For breeding, females and males of both species were housed in the ratio of 2 : 1. Dams were removed from breeding cages when visibly pregnant (approximately 12–14 days of gestation) and individually housed. Mouse pups were weaned at 22 days of age and housed with their siblings in cages of three to five mice. All procedures were performed according to guidelines developed by the Canadian Council on Animal Care with protocols approved by the Carleton and McGill Universities Committee on Animal Care. All experiments were performed by individuals unaware of the developmental history of the animals.

Adoption Study

Crossfostering was performed within 6 h following the birth of the last pup and completed within 15 min of having originally disturbed the litters. Pups born to BALB/c mothers were crossfostered onto either other BALB/c mothers (B-B) or C57BL/6 mothers (B-C57). Likewise, pups born to C57BL/6 mothers were crossfostered onto either other C57BL/6 mothers (C57-C57) or low BALB/c mothers (C57-B). As we have observed that both BALB/cByJ and C57BL/6ByJ will care for their own pups (defined in terms of their LG-ABN) as readily as they care for fostered pups (data not shown), the adoption study was performed so that whole litters of mice were crossfostered. Thus, although fostering entire litters has been reported to disturb maternal behaviors (Maccari et al, 1995), such an outcome was not observed among the BALB/cByJ or C57BL/6ByJ mice.

Receptor Autoradiography

For all experiments, brains were obtained from male adult (between 120 and 140 days of age) offspring of BALB/c and C57BL/6 mothers (_n_=5–6/group) by rapid decapitation less than 1 min following removal from the home cage. Brains were quickly removed, frozen in −70°C isopentane and stored at −80°C. Brains were sectioned in the coronal plane at 15 μM and sections were thaw-mounted onto gel-coated slides, which were then stored at −80°C until the time of assay.

Nonselective BZ receptor binding (for _α_1, _α_2, _α_3, _α_5 subtypes, previously known as type II) was examined using a procedure described by Bureau and Olsen (1993). Slides were thawed and preincubated in assay buffer (0.17 M Tris-HCl, pH 7.4) for 30 min at 4°C. The slides were then incubated with a saturating 0.5 nM concentration of [3H]flunitrazepam (84.5 Ci/mmol, New England Nuclear, Boston, MA) in assay buffer for 60 min at 4°C. Nonspecific, background binding was determined in parallel sections using 1 μM clonazepam. Post-assay washes (2 × 30 s) were performed using assay buffer. The sections were left to dry overnight and were then apposed to tritium-sensitive Ultrafilm (Amersham Canada Inc., Montréal, Canada) along with [3H] microscales for 14 days.

BZ _α_1-selective receptor binding (previously known as type I) was quantified using [3H]zolpidem (50.8 Ci/mmol, New England Nuclear) following the procedures described in Ruano et al (1993). Slides were thawed and preincubated in 50 mM Tris-HCl assay buffer (120 mM NaCl, 5 mM KCl, pH 7.4) for 30 min at room temperature. Sections were then incubated in assay buffer containing a saturating 5 nM concentration of [3H]zolpidem (5 nM) for 30 min at 4°C. Nonspecific binding was determined in parallel sections incubated with buffer containing 50 μM of cold zolpidem (purchased from RBI, Natick, MA). Sections were then washed (2 × 3 min) in ice-cold assay buffer, rinsed in cold, distilled water and dried rapidly under a stream of cold air. Autoradiograms were generated by apposing slides to tritium-sensitive Ultrafilm with the appropriate [3H] microscales for a period of 14 days, a period that provided for linearity of microscales and maximal binding.

Autoradiograms were analyzed by obtaining optical densities (expressed as mean±SEM in fmol/mg protein) determined by computer-assisted densitometry using an MCID image analysis system (Imaging Research Inc., St Catherines, Ontario, Canada) and low-activity tritium standards using the rat brain atlas of Franklin and Paxinos (1997). There were 3–4 sections per region used in the analysis for each animal and the mean from these values was then used in the statistical analysis.

In Situ Hybridization

Brains were obtained from adult male animals by rapid decapitation shortly (<20 s) following the removal of the animal from the home cage. Brains were frozen in isopentane maintained on dry ice and 15 μM coronal sections were prepared under RNase-free conditions and stored at −80°C. In preparation for the hybridization experiments, sections were prefixed in a 4% paraformaldehyde solution for 10 min. Sections were then washed in 2 × SSC buffer (2 × 5 min) and in 0.25% acetic anhydride and 0.1 M triethanolamine solution (pH 8.0; 1 × 10 min). Sections were then dehydrated using a 50–100% ethanol gradient, placed in chloroform for 10 min, followed by rehydration in 95% ethanol. Sections were then incubated overnight at 37°C with 75 μl/section of hybridization buffer containing 50% dionized formamide, 10 mM dithiothreitol, 10 mM Tris (pH 7.5), 600 mM sodium chloride, 1 mM EDTA, 10% dextran sulfate, 1 × Denhardt's solution, 100 μg salmon sperm DNA, 100 μg/ml yeast tRNA, with 1 × 106 CPM 35S-ddATP-labelled oligonucleotide probe. Oligonucleotide probes (_β_1: 5′ … GGG GTC ACC CCT GGC TAA GTT AGG GGT ATA GCT GGT TGC TGT AGG … 3′; _γ_2: 5′ … GTC ATA GCC ATA TTC TTC ATC CCT CTC TTG AAG GTG GGT GGC … 3′) (Wisden et al, 1992) were synthesized (Beckman 1000 DNA Synthesizer, Beckman, USA) and labelled using a DNA 3′-end-labelling kit (Boehringer Mannheim, USA). Note, the _γ_2 oligonucleotide sequence used in this study recognizes both _γ_2L and _γ_2S variants of the _γ_2 subunit. Preliminary studies using a scrambled version of the _α_1 and _γ_2 probes yielded no specific signal on brain sections (data not shown). Following hybridization, slides were washed for 4 × 30 min in 1 × SSC at 55°C, rinsed briefly in water, dried and apposed to Hyperfilm for 21 days along with sections of 35S-labelled standards prepared with known amounts of 35S in a brain paste. The hybridization signal within various brain regions was quantified using densitometry with an image analysis system (MCID, St Catherines, Ontario, Canada). The data are presented as arbitrary optical density (absorbance) units following correction for background. The anatomical level of analysis was verified using the rat brain atlas of Franklin and Paxinos (1997) with Nissl staining of sections following autoradiography.

Behavioral Testing

While numerous tests of anxiety are available that differentiate C57BL/6ByJ and BALB/cByJ mice, the step-down exploration paradigm is particularly effective. In this paradigm, mice are placed on a small platform at the center of an elevated open field, and the latency to place two or four legs off the platform is measured (Anisman et al, 2001; Sakic et al, 1994). Ordinarily, BALB/cByJ mice are reluctant to step off the platform (essentially a home base) and explore the remainder of the open field, whereas C57BL/6ByJ do so readily; the effect is eliminated with diazepam (Anisman et al, 2001).

Mice (∼70 days of age) comprised BALB/cByJ mice raised by a BALB/cByJ (_n_=14) or a C57BL/6ByJ dam (_n_=16), or C57B/6ByJ mice raised by the same strain (_n_=19) or a BALB/cByJ (_n_=21) dam. Mice were placed on a platform (12.5 × 8.50 × 2 cm) situated at the center of a 80 × 60 cm table-top located within a dimly lit room, and the latency to step down was monitored. The platform was covered with a thin removable, black rubber sleeve that allowed the animal to grip the surface. To avoid the influence of urine or other odors, the sleeve was changed for each animal. Mice were gently placed on the platform, and the latencies to stretch attend (mice remain fully on the platform, but extend their head body, only to withdraw again), place the two forelegs on the table-top, and place all four legs on the table-top were recorded. If a mouse did not step off the platform within 300 s, the trial was terminated. Mice received three such trials at 1 min intervals between trials. Even though the platform was quite low, highly anxious mice ordinarily remain on the platform for extended periods, whereas less anxious animals step off and explore.

Statistical Analysis

The data were analyzed using a two-way (Group × Region) analysis of variance with Tukey post hoc tests where appropriate.

RESULTS

Benzodiazepine Receptor Binding

There was a significant Group and Group × Region interaction effect (F(9,54)=2.91; p<0.01) for [3H]flunitrazepam binding (Figure 1). Post hoc analysis revealed significantly increased central BZ receptor levels in C57BL/6 mice in the central, basolateral, and lateral nuclei of the amygdala. There were no significant group differences found in the medial prefrontal cortex, the hippocampus, or the locus coeruleus. [3H]flunitrazepam binding is nonselective. In order to examine the relevant receptor subtype, we examined the _α_1 subtype-selective BZ binding using [3H]zolpidem (Ruano et al, 1993). A significant Group × Region interaction effect (F(9,54)=2.53; p<0.02) for [3H]zolpidem binding was observed (Figure 2). Post hoc analysis showed significantly increased _α_1 subtype-selective central BZ receptor levels in C57BL/6 mice in the central and lateral nuclei of the amygdala as well as the locus coeruleus. There were no significant group differences found in the medial prefrontal cortex, the hippocampus, and the basolateral nuclei of the amygdala.

Figure 1

figure 1

Mean±SEM levels of central benzodiazepine receptor ([3H]flunitrazepam) binding (in fmol/mg protein) in various brain regions (_n_=6 animals per group). mPFC, medial prefrontal cortex; DG, dentate gyrus; CA1-3, hippocampal Ammon's horn; BLnA, basolateral nucleus of the amygdala; LnA, lateral nucleus of the amygdala; CnA, central nucleus of the amygdala; LC, locus coeruleus. ***p<0.005; **p<0.01; *p<0.05.

Full size image

Figure 2

figure 2

Mean±SEM levels of central benzodiazepine (type I) receptor ([3H]zolpidem) binding (in fmol/mg protein) in various brain regions (_n_=6 animals per group). [FC2], frontal cortex area 2; for other abbreviations, see the caption to Figure 1. **p<0.005; *p<0.05. (b) Representative photomicrograph depicting [3H]zolpidem binding in the central (CAN) and basolateral (BLN) nuclei of the amygdala in BALB/cByJ and C57BL/6ByJ mice.

Full size image

GABAA Subunit mRNA Expression

There was a significant Group × Region interaction effect (F(9,54)=4.55; p<0.0002) for _α_1 mRNA expression (Figure 3a). Post hoc analysis revealed significantly increased _α_1 mRNA levels in C57BL/6 mice in the locus coeuruleus and decreased expression in the CA1 region of the hippocampus compared to BALB/c mice. There were no significant group differences in the medial prefrontal cortex, the CA2, CA3, and dendate gyrus regions of the hippocampus or in the central, lateral, and basolateral nuclei of the amygdala. Analysis of the _γ_2 mRNA data (Figure 3b) also showed a significant group and Group × Region interaction effect (F(9,54)=5.28; p<0.0001). In this case however, _γ_2 mRNA expression was significantly increased in the C57BL/6 mice in the central nucleus of the amygdala. No other comparisons approached significance.

Figure 3

figure 3

(a) Mean±SEM levels of mRNA for the _α_1 subunit of GABAA receptor in various brain regions expressed as optical density units from autoradiograms from in situ hybridization studies with 35S-labelled oligonucleotide probes (_n_=6 animals per group). *p<0.05. (b) Mean±SEM levels of mRNA for the γ2 subunit of GABAA receptor in various brain regions expressed as optical density units from autoradiograms from in situ hybridization studies with 35S-labelled oligonucleotide probes (_n_=6 animals per group). *p<0.05. (c) Representative photomicrograph depicting the _γ_2 subunit mRNA expression in the central (CAN) nucleus of the amygdala in BALB/cByJ and C57BL/6ByJ mice. For abbreviations, see the caption to Figures 1 and 2.

Full size image

Adoption Study

In the adoption study, we analyzed expression of the mRNAs for the _γ_2 subunits in various brain regions. The results revealed a highly significant effect for rearing mother (F(1,16)=60.9; p<0.0001) but not biological mother (F(1,16)=1.59; _p_=0.23; Figure 4) in the central nucleus of the amygdala. There was no significant main effect of biological mother in any region examined. Thus, _γ_2 subunit mRNA expression in animals born to BALB/c mothers but reared by C57BL/6 dams was indistinguishable from that of the normal offspring of C57BL/6 mothers. The reverse was also true for pups born to C57BL/6 dams but reared by BALB/c mothers.

Figure 4

figure 4

Mean±SEM levels of mRNA for the _γ_2 subunit of GABAA receptor in various brain regions expressed as optical density units from autoradiograms from in situ hybridization studies with 35S-labelled oligonucleotide probes (_n_=5 animals per group) in the biological offspring of BALB/c mothers crossfostered to either BALB/c (B-B) or C57BL/6 (B-C57) mothers and the biological offspring of C57BL/6 mothers crossfostered to either C57BL/6 (C57-C57) or BALB/c (C57-B) mothers. For abbreviations, see the caption to Figures 1 and 2. *p<0.001.

Full size image

Behavioral Data

Figure 5 shows the latency to place two legs on the table-top, and the latency to step fully (all four legs) off the platform and onto the table-top. The analysis of the latency to place two legs onto the table-top varied as a function of the Strain × Dam interaction (F(1,66)=6.52; _p_=0.01). The post hoc tests indicated that the latencies to place two legs off the platform onto the table-top were longer in BALB/cByJ mice raised by a BALB/dam than among C57BL/cByJ mice raised by either the same or a different strain. However, BALB/cByJ mice raised by a C57BL/6ByJ dam exhibited latencies that did not differ from those of normal C57BL/6ByJ mice. The longer response latencies ordinarily evident in BALB/cByJ were eliminated if these mice were raised with a C57BL/6ByJ dam. However, C57BL/6ByJ mice raised by a BALB/cByJ dam showed no resemblance to the rearing, and instead were fully comparable to C57BL/6ByJ mice born to and reared by C57BL/6ByJ mothers. Latencies to place all four legs off the platform indicated that the response latencies were longer in BALB/cByJ than in C57BL/6ByJ mice (F(1,66)=95.58; p<0.01) and were also slower in mice raised by a BALB/cByJ than a C57BL/6ByJ dam (F(1,66)=381; p<0.05). Post hoc analyses confirmed that the behavior of BALB/cByJ mice raised by a C57BL/6ByJ dam was intermediate between the two strains raised by the same strain dam (ie significantly lower than BALB/cByJ mice reared by a BALB/cByJ mother, and greater than in either group of C57BL/6ByJ animals).

Figure 5

figure 5

Mean±SEM latency of BALB/cByJ mice raised by a BALB/cByJ (_n_=14) or a C57BL/6ByJ dam (_n_=16; BALB/BALB and C57/BALB, respectively) or C57B/6ByJ mice raised by the same strain (_n_=19) or a BALB/cByJ (_n_=21) dam (C57/C57 and C57/BALB, respectively). Mice were placed on a platform situated at the center of an open field and the latency to step down (with two or four legs) was measured. **p<0.001; *p<0.01 from all other groups.

Full size image

DISCUSSION

BALB/c mice show decreased levels of BZ binding in the central, lateral, and basolateral nuclei of the amygdala as measured by either [3H]flunitrazepam or [3H]zolpidem (Figures 1 and 2). Flunitrazepam is a nonselective ligand for the central BZ receptor. In contrast, zolpidem is highly selective for the _α_1 receptor subtype of the central BZ receptor. As in the previous studies, differences between BALB/c and C57BL/6 mice in BZ binding were highly specific to the amygdala (Hode et al, 2000). In this study, as in the previous research of Hode et al (2000), the most consistent differences in BZ binding emerged in the central nucleus of the amygdala. In the current study, this effect was most prominent using the _α_1 subtype-selective BZ receptor ligand zolpidem.

The α subunit forms the GABA binding site and it appears as though the interface between the α and γ subunits forms the BZ receptor site. Thus, the presence of the γ subunit is critical in defining the BZ site, which might explain the absence of direct overlap between the _α_1 mRNA expression and that of [3H]flunitrazepam binding. While the presence of the γ subunits appears to define BZ receptor binding, it is the nature of the α subunit that determines the BZ receptor pharmacology (McKernan et al, 1991; Barnard et al, 1998; Mehta and Ticku, 1999). Receptors containing the _α_1 subunit exhibit a high affinity for zolpidem. However, expression of other α subunits, notably _α_2, _α_3, and _α_5, results in reduced affinity for zolpidem (McKernan et al, 1991; Barnard et al, 1998; Mehta and Ticku, 1999). The expression of the mRNAs for these subunits was not examined here. Moreover, immunoprecipitation assays are required to verify exactly which subunits are incorporated into functional GABAA receptor sites. The possibility of alterations in the _α_5 subunit will be especially interesting considering a recent report of the role of this subunit in fear conditioning (Bailey et al, 2002; Crestani et al, 2002).

BZ receptor agonists exert anxiolytic effects via their actions at a number of limbic areas. However, the evidence so far is strongest at the level of amygdala complex (Pitkanen et al, 1997) comprising the lateral, basolateral, and the central nuclei of the amygdala. Direct administration of BZs into the basolateral or central nuclei of the amygdala results in an anxiolytic effect (Hodges et al, 1987; Pesold and Treit, 1995; Gonzalez et al, 1996). Recent studies have focused on the effects of intra-amygdaloid infusion of either GABAA or BZ receptor agonists or antagonists on fear conditioning. The results show potent effects of such treatments on the acquisition (amnesic effects) and expression (anxiolytic effects) of conditioned fear responses and underscore the importance of the amygdala as a critical target for the effects of GABAA and BZ receptor agonists (Brioni et al, 1989; Tomaz et al, 1993; Helmstetter and Bellgowan, 1994; Dickinson-Anson and McGaugh, 1997; Muller et al, 1997; Da Cunha et al, 1999). Hence, if one assumes the presence of an endogenous agonist (see Da Cunha et al, 1999), the differences in BZ binding might serve as a neural substrate for the increased fearfulness of the BALB/c strain.

The group differences in BZ binding in the central nucleus of the amygdala were associated with effects at the level of GABAA receptor subunit expression. The _γ_2 subunit mRNA expression was significantly elevated in C57BL/6 mice compared to BALB/c animals and, among the areas examined, differences were unique to the amygdala (see Figure 2b). Expression of the _α_1 subunit mRNA differed as a function of strain in the CA1 region of the hippocampus and the locus coeruleus; however, such differences did not map onto those in BZ binding. In contrast, the differences in _γ_2 subunit expression could serve as a mechanism for the strain differences in BZ receptor binding in the central nucleus of the amygdala. The inclusion of a _γ_1 or _γ_2 subunit in the GABAA receptor complex appears to define the presence of a BZ receptor site. Point mutations have been identified within both subunits that are sufficient to eliminate BZ receptor binding (Amin et al, 1997; Buhr and Sigel, 1997; Buhr et al, 1997; Wingrove et al, 1997), and animals bearing a null mutation of the _γ_2 subunit show approximately an 85% loss of [3H]flunitrazepam binding (Gunther et al, 1995).

These findings suggest that variations in maternal care might alter GABAA receptor subunit expression in the amygdala and thus BZ/GABAA receptor function. The results of the crossfostering study suggest a direct relationship between maternal care and individual differences in GABAA receptor subunit expression. Levels of _γ_2 subunit mRNA in animals born to BALB/c mothers but reared by C57BL/6 dams were indistinguishable from that of the normal offspring of C57BL/6 mothers. The reverse was also true for pups born to C57BL/6 dams but reared by BALB/c mothers. These findings imply a postnatal, maternal effect on GABAA receptor development in these mice strains and are consistent with previous crossfostering studies examining behavioral outcomes in these strains (Zaharia et al, 1996). Moreover, fear-related behavior in BALB/c mice is altered by postnatal handling, which is known to increase maternal licking/grooming (Lee and Williams, 1975; Liu et al, 1997).

As previously reported (Anisman et al, 2001; Belzung and Griebel, 2001; Beuzen and Belzung, 1995; Oliverio et al, 1973; Peeler and Nowakowski, 1987), BALB/cByJ mice exhibited greater anxiety-like behavior relative to C57BL/6ByJ mice upon testing in the step-down exploration task. We (Zaharia et al, 1996) previously reported that the poor performance of BALB/cByJ mice in a Morris water-maze spatial learning test, which is subject to modification by anxiolytic treatment, is affected by the strain of the rearing mother. In contrast, the more proficient Morris water-maze performance of the less fearful C57BL/6ByJ was not altered by the strain of the rearing dam. Similarly, in the present investigation, when BALB/cByJ mice were crossfostered onto a C57BL/6ByJ dam, the anxiety responses characteristic of BALB/cByJ mice were largely attenuated. However, among C57BL/6ByJ mice, the relatively low levels of anxiety ordinarily evident were not increased when mice of this strain were reared by a BALB/cByJ dam. The epigenetic effects of postnatal, crossfostering apparent in the BALB/cByJ mice may in fact occur in C57BL/6ByJ mice, but over different time periods. Francis et al (2003) found that C57BL/6ByJ mice fostered to BALB/cByJ dam over both the prenatal period (with transplantation of C57BL/6ByJ embryos into BALB/cByJ mothers) and the postnatal period (with crossfostering identical to our procedure) did indeed resemble normal BALB/cByJ on measures of open-field exploration, Morris water-maze learning, plus maze behavior, and prepulse inhibition. However, this effect occurred only if the BALB/cByJ maternal environment prevailed both pre- and postnatally. Similar to our findings, the C57BL/6ByJ mice crossfostered to BALB/cByJ dams after delivery from BALB/cByJ mothers were unaffected. In this study and others (Zaharia et al, 1996), BALB/cByJ mice fostered postnatally to C57BL/6ByJ mothers did indeed resemble C57BL/6ByJ mice on behavioral measures of fear. These studies possibly reflect a fascinating genomic constraint on the effect of environmental influence, and its timing, on phenotype.

The behavioral data also reveal some dissociation between GABAA receptor subunit expression in the amygdala and fear behavior. However, in the current study, we examined only the _α_1 and _γ_2 subunits. GABAA receptor function is determined through a substantially greater range of subunits, including the _α_5 subunit that has been linked to fear conditioning (Bailey et al, 2002; Crestani et al, 2002). Moreover, the expression of such complex behavioral patterns will inevitably involve a diverse neural circuitry of which the amygdaloid GABAA receptor is but one component. It is interesting, however, that unlike the behavioral findings, the expression of mRNA encoding for the _γ_2 subunit was altered in C57BL/6ByJ reared by BALB/cByJ mothers during the postnatal period. Obviously, individual features of phenotype will emerge in response to differing gene × environment interactions at different stages in development.

Strain differences in mice are commonly assumed to reflect genomic variation. While this is undoubtedly true, the relationship between genotype and phenotype is less obvious than often thought. Simply put, the existence of strain differences in any specific trait does not imply that such variation in phenotype is directly and invariably linked to differences in genotype. Certainly, one possibility is that differences in maternal behavior might be directly associated with genomic variation, and that the differences in BZ/GABAA receptor development and behavioral fearfulness might then emerge as a function of altered maternal care, and not as a direct effect of genomic variation on fearfulness. Such maternally mediated, indirect genetic effects are considered common in evolutionary biology (eg Hunt and Simmons, 2002 and references therein). Such effects are thought to form the basis whereby traits without heritable variation can evolve because of the inheritable environmental variation provided through indirect genetic effects. In this case, the inheritable environmental variation is that of strain differences in maternal care. Of course, the differences in maternal behavior could also be secondary to some other trait, and so on. The critical question concerns the mechanisms by which genomic factors result in phenotypic variation, and this will no doubt involve a series of complex gene × environment interactions.

There are certainly implications for human studies attempting to quantify genetic and environmental contributions to individual differences. While the importance of gene × environment interactions is often considered, research commonly relies on linear regression models that provide estimates of the strength of association between input factors and defined outcomes. Such measures largely focus on ‘main effects’ estimating the percentage of the variance accounted for by ‘genetic’ vs ‘environmental’ factors. In the case of the behavioral differences between BALB/c and C57BL/6 mice, information on the strain of the animals would be sufficient to distinguish statistically one group from the other, as virtually almost BALB/c mice behave differently than do C57BL/6 animals on tests of fearfulness or learning/memory. Knowledge of differences in the postnatal environment, such as variations in maternal behavior, would add absolutely nothing to the ability of the equation to predict differences in behavior. A linear regression analysis of variation in, say, timidity would yield no insight whatsoever into the potential importance of environmental factors—partitioning of the variance would slide entirely onto the so-called ‘genetic’ variation reflected in the identification of the strain, despite the fact that the maternal styles of these strains are markedly different from one another. Although the addition of the environmental variables ought to increase the predictive value, within a linear model these variables would be considered statistically unimportant (or redundant) in determining the variance associated with phenotype. Hence we might be tempted to conclude that the differences in fearfulness emerge largely, if not exclusively, from genetic factors. Precisely, this form of interpretation has given rise to such remarkably specious debates as to ‘whether parents really matter?’ In the case of the BALB/c and C57BL/6 mice, there is considerable phenotypic plasticity such that maternal care does indeed alter behavioral responses to stress and BZ/GABAA receptor development in brain regions that mediate the expression of fearfulness. For these animals, parents do matter. It is tempting to think that, in this case, the effects of genomic variation are mediated by strain differences in maternal care, thus blurring considerably the distinction between gene and environment. Given that phenotypic expression across strains of mice is influenced by test conditions, even using standardized procedures (Crabbe et al, 1999; Wahlsten et al, 2003), it should not be surprising to find that profound behavioral experiences, especially those encountered early in life, would impact on emotionality and on the presumed neurochemical substrates for anxiety-like states.

References

Download references

Acknowledgements

This research was supported by grants from the Canadian Institutes of Health Research to MJM and to HA. CC is a graduate fellow and a recipient of a Canadian Institutes of Health Research Doctoral Research Award. MJM holds a Senior Investigator award from the Canadian Institutes of Health Research and is a Lieber Investigator of the National Alliance for Research in Schizophrenia and Affective Disorders. HA holds a Canada Research Chair award.

Author information

Authors and Affiliations

  1. Developmental Neuroendocrinology Laboratory, Douglas Hospital Research Centre, McGill University, Montréal, Canada
    Christian Caldji, Josie Diorio & Michael J Meaney
  2. Institute of Neuroscience, Carleton University, Ottawa, Ontario, Canada
    Hymie Anisman

Authors

  1. Christian Caldji
    You can also search for this author inPubMed Google Scholar
  2. Josie Diorio
    You can also search for this author inPubMed Google Scholar
  3. Hymie Anisman
    You can also search for this author inPubMed Google Scholar
  4. Michael J Meaney
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toMichael J Meaney.

Rights and permissions

About this article

Cite this article

Caldji, C., Diorio, J., Anisman, H. et al. Maternal Behavior Regulates Benzodiazepine/GABAA Receptor Subunit Expression in Brain Regions Associated with Fear in BALB/c and C57BL/6 Mice.Neuropsychopharmacol 29, 1344–1352 (2004). https://doi.org/10.1038/sj.npp.1300436

Download citation

Keywords