A functional PtdIns(3)P-binding motif (original) (raw)

Nature volume 394, pages 433–434 (1998)Cite this article

Abstract

Treating cells with the phosphatidylinositol-3-OH kinase (PI(3)K) inhibitor wortmannin causes the dissociation of the early-endosomal antigen EEA1 from early endosomes1. EEA1 from cytosolic extracts binds to liposomes containing phosphatidylinositol-3-phosphate (PtdIns(3)P), the major product of PI(3)K in yeast and mammalian cells1,2. Here we show that a RING zinc-finger domain at the carboxy terminus of EEA1, previously identified and named the ‘FYVE’ domain3, binds directly and specifically to PtdIns(3)P. This indicates that proteins containing this motif may be downstream effectors of PI(3)K in yeast and mammalian cells.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

References

  1. Patki, V.et al. Proc. Natl Acad. Sci. USA 94, 7326–7330 (1997).
    Google Scholar
  2. Auger, K. R.et al. Cell 57, 167–175 (1989).
    Google Scholar
  3. Stenmark, H.et al. J. Biol. Chem. 271, 24048–24054 (1996).
    Google Scholar
  4. Weisman, L. S. & Wickner, W. J. Biol. Chem. 267, 618–623 (1992).
    Google Scholar
  5. Piper, R. C., Cooper, A., Yang, H. & Stevens, T. H. J. Cell. Biol. 131, 603–617 (1995).
    Google Scholar
  6. Komada, M., Masaki, R., Yamamoto, A. & Kitamura, N. J. Biol. Chem. 272, 20538–20544 (1997).
    Google Scholar
  7. Zheng, Y.et al. J. Biol. Chem. 271, 33169–33172 (1996).
    Google Scholar
  8. Schu, P. V.et al. Science 260, 88–91 (1993).
    Google Scholar
  9. Vanhaesebroeck, B.et al. Trends Biochem. Sci. 22, 267–272 (1997).
    Google Scholar
  10. Klarlund, J. K.et al. Science 275, 1927–1930 (1997).
    Google Scholar
  11. Gaullier, J.-M.et al. Nature 394, 432–433 (1998).
    Article ADS CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Program in Molecular Medicine and Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01605, Massachusetts, USA
    Varsha Patki, Deirdre C. Lawe & Silvia Corvera
  2. Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, 01605, Massachusetts, USA
    Joseph V. Virbasius & Anil Chawla

Authors

  1. Varsha Patki
    You can also search for this author inPubMed Google Scholar
  2. Deirdre C. Lawe
    You can also search for this author inPubMed Google Scholar
  3. Silvia Corvera
    You can also search for this author inPubMed Google Scholar
  4. Joseph V. Virbasius
    You can also search for this author inPubMed Google Scholar
  5. Anil Chawla
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Patki, V., Lawe, D., Corvera, S. et al. A functional PtdIns(3)P-binding motif.Nature 394, 433–434 (1998). https://doi.org/10.1038/28771

Download citation

Associated content