A rat monoclonal antibody against mouse α and β interferon of all molecular weight species (original) (raw)
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- Published: 15 April 1982
Nature volume 296, pages 664–665 (1982)Cite this article
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Abstract
Monoclonal antibodies have proven to be invaluable reagents for the characterization and purification of human interferons (IFN). Hybridoma lines secreting monospecific antibodies against human IFN-α and IFN-β have been isolated, and these antibodies have shown no cross-reactivity between the two interferons1–4. Murine interferons, when analysed by polyacrylamide gel electrophoresis, have two major migration patterns, one in the 28,000–35,000 (28–35 K) molecular weight (_M_r) region and the second in the 22 K region5–8. Based on some homology of the N-terminal amino acid sequence with human interferons, the 28 K and 35 K species are classified as mouse IFN-β and the 22 K species as mouse IFN-_α_9. Minor species have also been described but their relationship to IFN-α and IFN-β is unknown10,11. We have now established a stable hybridoma line secreting rat antibodies to mouse virus-induced interferon. The monoclonal antibodies are of the IgG class, with binding activity to all the different _M_r specied, as demonstrated in an enzyme-linked immunosorbent assay (ELISA) and by affinity chromatography. In addition, the antiviral activity of all the _M_r species was neutralized by the antibody but the neutralizing activity was much weaker than the binding activity. This indicates the presence of a common antigenic site for all mouse α and β interferon species.
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Authors and Affiliations
- Institut Curie, Centre Universitaire, Orsay Cedex, 91405, France
Daniela Männel, E. De Maeyer, A. Cachard & Jaqueline De Maeyer-Guignard - Institut Pasteur, 25, rue du Roux, Paris Cedex, 75015, France
R. Kemler
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- Daniela Männel
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Männel, D., Maeyer, E., Kemler, R. et al. A rat monoclonal antibody against mouse α and β interferon of all molecular weight species.Nature 296, 664–665 (1982). https://doi.org/10.1038/296664a0
- Received: 14 December 1981
- Accepted: 05 March 1982
- Issue Date: 15 April 1982
- DOI: https://doi.org/10.1038/296664a0