An inositol tetrakisphosphate-containing phospholipid in activated neutrophils (original) (raw)
- Letter
- Published: 01 July 1988
- Anna L. Harris1,
- Barbara L. Thompson1 na1,
- Palmer Taylor1 na1 &
- …
- Larry A. Sklar1
Nature volume 334, pages 353–356 (1988)Cite this article
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Abstract
Inositol (l,4,5)trisphosphate (InsP3)1 and tetrakisphosphate (InsP4)2 have been observed in a variety of cell types and have been proposed to play roles in the receptor-mediated rise in intracellular Ca2+ (refs 2, 3). Recently, they have been shown to act synergistically in the activation of a Ca2+-dependent K+ channel in lacrimal acinar cells3. InsP3 is the product of phospholipase C (PLC) action on phosphatidylinositol 4,5-bisphosphate (PtdInsP2), whereas InsP4 is believed to arise from phosphorylation of InsP3 by a cytosolic kinase4. Although sought as a source for InsP4, PtdInsP3 has not been identified in any specific cell type2. There were early reports of InsP4-containing phospholipids in crude extract from bovine brain5, but this finding was later withdrawn6. Recently, however, a membrane-bound enzyme (Type 1 PI kinase) which adds phosphate onto the 3 position of inositol phospholipids has been identified7 and the phosphatidylinositol-3-phosphate (PtdIns(3)P) product characterized. This suggests that several forms of phosphoinositides may exist and could be precursors for some of the variety of soluble inositol phosphate products which have been reported in recent years. Here we report the appearance of another novel phosphoinositide containing four phosphates, phosphatidylinositol trisphosphate (PtdInsP3) which we find only in activated but not in unstimulated neutrophils from human donors.
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Author notes
- Barbara L. Thompson and Palmer Taylor: Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA
Authors and Affiliations
- Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA, 92037, USA
Alexis E. Traynor-Kaplan, Anna L. Harris, Barbara L. Thompson, Palmer Taylor & Larry A. Sklar
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- Alexis E. Traynor-Kaplan
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Traynor-Kaplan, A., Harris, A., Thompson, B. et al. An inositol tetrakisphosphate-containing phospholipid in activated neutrophils.Nature 334, 353–356 (1988). https://doi.org/10.1038/334353a0
- Received: 03 May 1988
- Accepted: 06 June 1988
- Published: 01 July 1988
- Issue Date: 28 July 1988
- DOI: https://doi.org/10.1038/334353a0
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