Normal human mammary epithelial cells spontaneously escape senescence and acquire genomic changes (original) (raw)

• Shay, J. W., Wright, W. E. & Werbin, H. Toward a molecular understanding of human breast cancer: a hypothesis. Breast Cancer Res. Treatment. 25 83–94 (1993).
  Article CAS Google Scholar
• Hayflick, L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37, 614–636 (1965).
  Article CAS Google Scholar
• Hammond, S. L., Ham, R. G. & Stampfer, M. R. Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended passage with pituitary extract. Proc. Natl Acad. Sci. USA 81, 5435–5439 (1984).
  Article ADS CAS Google Scholar
• Foster, S. A. & Galloway, D. A. Human papillomavirus type 16 E7 alleviates a proliferation block in early passage human mammary epithelial cells. Oncogene 12, 1773–1779 (1996).
  CAS PubMed Google Scholar
• Huschtscha, L. I. et al. Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells. Cancer Res. 58, 3508–3512 (1998).
  CAS PubMed Google Scholar
• Kiyono, T. et al. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396, 84–88 (1998).
  Article ADS CAS Google Scholar
• Meyer, K. M., Hess, S. M., Tlsty, T. D. & Leadon, S. A. Human mammary epithelial cells exhibit a differential p53-mediated response following exposure to ionizing or UV light. Oncogene 18, 5792–57805 (1999).
  Article Google Scholar
• Walen, K. H. & Stampfer, M. R. Chromosome analyses of human mammary epithelial cells at stages of chemical-induced transformation progression to immortality. Cancer Genet. Cytogenet. 37, 249–261 (1989).
  Article CAS Google Scholar
• Brenner, A. J., Stampfer, M. R. & Aldaz, C. M. Increased p16 expression with first senescence arrest in human mammary epithelial cells and extended growth capacity with p16 inactivation. Oncogene 17, 199–205 (1998).
  Article CAS Google Scholar
• Dimri, G. P. et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl Acad. Sci. USA 92, 9363–9367 (1995).
  Article ADS CAS Google Scholar
• Pignolo, R. J., Rotenberg, M. O. & Cristofalo, V. J. Alterations in contact and density-dependent arrest state in senescent WI-38 cells. In Vitro Cell. Dev. Biol. Anim. 30A, 471–476 (1994).
  Article CAS Google Scholar
• Shay, J. W. & Wright, W. E. Quantitation of the frequency of immortalization of normal human diploid fibroblasts by SV40 large T-antigen. Exp. Cell Res. 184, 109–118 (1989).
  Article CAS Google Scholar
• Taylor-Papadimitriou, J. et al. Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J. Cell Sci. 94, 403–413 (1989).
  PubMed Google Scholar
• Foster, S. A., Wong, D. J., Barrett, M. T. & Galloway, D. A. Inactivation of p16 in human mammary epithelial cells by CpG island methylation. Mol. Cell. Biol. 18, 1793–1801 (1998).
  Article CAS Google Scholar
• Lansdorp, P. M. et al. Heterogeneity in telomere length of human chromosomes. Hum. Mol. Genet. 5, 685–691 (1996).
  Article CAS Google Scholar
• van Steensel, B., Smogorzewska, A. & de Lange, T. TRF2 protects human telomeres from end-to-end fusions. Cell 92, 401–413 (1998).
  Article CAS Google Scholar
• Stampfer, M. R. et al. Gradual phenotypic conversion associated with immortalization of cultured human mammary epithelial cells. Mol. Biol. Cell 8, 2391–2405 (1997).
  Article CAS Google Scholar
• Karlseder, J., Broccoli, D., Dai, Y., Hardy, S. & de Lange, T. p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2. Science 283, 1321–1325 (1999).
  Article CAS Google Scholar
• Artandi, S. E. et al. Telomere dysfunction promotes non-reciprocal translocations and epithelial cancers in mice. Nature 406, 641–645 (2000).
  Article ADS CAS Google Scholar
• Chin, L. et al. p53 Deficiency rescues the adverse effects of telomere loss and cooperates with telomere dysfunction to accelerate carcinogenesis. Cell 97, 527–538 (1999).
  Article CAS Google Scholar
• Alcorta, D. A. et al. Involvement of the cyclin-dependent kinase inhibitor p16 (INK4A) in replicative senescence of normal human fibroblasts. Proc. Natl Acad. Sci. USA 92, 13742–13747 (1996).
  Article ADS Google Scholar
• Hara, E. et al. Regulation of p16CDKN2 expression and its implications for cell immortalization and senescence. Mol. Cell. Biol. 16, 859–867 (1996).
  Article CAS Google Scholar
• Burbano, R. R. et al. Cytogenetics of epithelial hyperplasias of the human breast. Cancer Genet. Cytogenet. 119, 62–66 (2000).
  Article CAS Google Scholar
• Pandis, N. et al. Chromosome abnormalities in bilateral breast carcinomas. Cytogenetic evaluation of the clonal origin of multiple primary tumors. Cancer 76, 250–258 (1995).
  Article CAS Google Scholar
• Berg, J. W. & Hutter, R. V. Breast cancer. Cancer 75, 257–269 (1995).
  Article CAS Google Scholar
• Stoeber, K. et al. Cdc6 protein causes premature entry into S phase in a mammalian cell-free system. EMBO J. 17, 7219–7229 (1998).
  Article CAS Google Scholar
• Wei, W. & Sedivy, J. M. Differentiation between senescence (M1) and crisis (M2) in human fibroblasts cultures. Exp. Cell Res. 253, 519–522 (1999).
  Article CAS Google Scholar
• Tlsty, T. D. et al. Potentiation of genomic instability in normal human mammary epithelial cells by an epigenetic event. J. Mammary Gland Biol. Neoplasia (in the press).