Peptide-induced conformational change of the class I heavy chain (original) (raw)

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• Published: 30 May 1991

Nature volume 351, pages 402–406 (1991)Cite this article

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Abstract

THERE is evidence that peptide ligands take part in the assembly of class I molecules1–8. In particular, addition of peptides to extracts of the mutant cells RMA-S and .174/T2, in which stable assembly of class I does not occur9–11, results in a conformational change in the class I heavy chain and stable association of the heavy chain with β2-microglobulin (β2m) (refs 1–3). Thus specific peptides may stabilize or induce a conformational change in the class I heavy chain that results in a rise in the binding affinity of the heavy chain for β2m (Fig. la). Here we show that peptides have two cooperative roles in class I assembly. Specific short peptides (9–10 amino acids) can induce folding of the heavy chain in the absence of β2m. Both short (nine amino acids) and longer sequences (15 amino acids) can stabilize preformed low-affinity complexes of heavy chain and β2_m_. To alter the conformation of free heavy chains, the peptides must be exactly the correct size, and they are found to correspond to the sequences isolated from infected cells12. This property may therefore be the basis for selection of epitopes presented in vivo.

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Authors and Affiliations

1. Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, 0X3 9DU, UK
   Tim Elliott, Vincenzo Cerundolo, John Elvin & Alain Townsend

Authors

1. Tim Elliott
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2. Vincenzo Cerundolo
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3. John Elvin
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4. Alain Townsend
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Elliott, T., Cerundolo, V., Elvin, J. et al. Peptide-induced conformational change of the class I heavy chain.Nature 351, 402–406 (1991). https://doi.org/10.1038/351402a0

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• Received: 19 February 1991
• Accepted: 23 April 1991
• Issue Date: 30 May 1991
• DOI: https://doi.org/10.1038/351402a0

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